This freshwater Tryblionella gracilis (or Nitzchia Tryblionella), length 109um, is poorly resolved under light microscopy. Even in Pleurax.
In the oblique image (photo 1) contrast was enhanced. Illumination was amber-filtered cool LED.
Photo 2 is plain darkfield, with unfiltered cool LED light. Turret condenser D position was used, and was watered to the bottom of the slide. The auxiliary lens holder was then rotated to partially block the illumination. Different angles of the light beam produced photos 3-5, oblique darkfield images. These images were only resized and cropped.
Tryblionella under darkfield and oblique darkfield
Tryblionella under darkfield and oblique darkfield
- Attachments
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- 1) Tryblionella gracilis 109um, Plan 40x0.65 water brightfield oblique.JPG (56.2 KiB) Viewed 1987 times
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- 2) Tryblionella gracilis 109um, Plan 40x0.65 water darkfield.jpg (43.17 KiB) Viewed 1987 times
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- 3) Tryblionella gracilis 109um, Plan 40x0.65 water oblique darkfield 1.JPG (44.46 KiB) Viewed 1987 times
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- 4) Tryblionella gracilis 109um, Plan 40x0.65 water oblique darkfield 2.JPG (44.33 KiB) Viewed 1987 times
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- 5) Tryblionella gracilis 109um, Plan 40x0.65 water oblique darkfield 3.JPG (47 KiB) Viewed 1987 times
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- Posts: 109
- Joined: Wed Dec 30, 2020 1:46 am
Re: Tryblionella under darkfield and oblique darkfield
Wow, rotating in that auxiliary lens holder to different degrees produced some really nice effects. Great images!
Re: Tryblionella under darkfield and oblique darkfield
Great images, thanks!
Re: Tryblionella under darkfield and oblique darkfield
Thanks, Jimbo and Nreerkay