Cheap Phase contrast part 2 : LOMO x20

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Cheap Phase contrast part 2 : LOMO x20

#1 Post by chrisimbee » Fri Apr 16, 2021 2:29 pm

Hello to you all,

I've received another Phase objective LOMO x20...and this time its the same series of my LOMO x40 that is a part of the LOMO phase contrast condenser KF-4.

And as i was disappointed with the x10 because i couldnt get nice images like I could with the x40 i was afraid ùy cheap technique might not work with the other objectives of the set.

And in fact this x20 works really fine !!! Even in critical illumination. I can use the same annulus I made for the x40 and located at the same place. All I have to do is to raise or lower the condenser until the size of the image of the annulus matches approx with the phase plate. (I use the phase telescope).

The image is much brighter and gives more colors depending how I raise the condenser. And its still proper phase contrast as i compared with my x40.

Here is a image extracted from a AVI I'll post on youtube. Its a vorticelli meeting another protozoa that was a bit too fast. The image will give you a rough idea of the brightness and contrast i could see..I'll post better pics soon..
16 avril 20 x20 essai 1.jpg
16 avril 20 x20 essai 1.jpg (100.79 KiB) Viewed 1385 times
Chris
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: Cheap Phase contrast part 2 : LOMO x20

#2 Post by chrisimbee » Fri Apr 16, 2021 2:40 pm

here is another photo : "classic" cheek cells in CP with x20 LOMO
16 avril x20 essay 2.jpg
16 avril x20 essay 2.jpg (74.88 KiB) Viewed 1384 times
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: Cheap Phase contrast part 2 : LOMO x20

#3 Post by chrisimbee » Sun Apr 18, 2021 6:23 pm

post-3045-0-93742800-1618748249.jpg
post-3045-0-93742800-1618748249.jpg (143.09 KiB) Viewed 1321 times
post-3045-0-93742800-1618748249.jpg
post-3045-0-93742800-1618748249.jpg (143.09 KiB) Viewed 1321 times
Attachments
post-3045-0-42998900-1618748354_thumb.jpg
post-3045-0-42998900-1618748354_thumb.jpg (43.55 KiB) Viewed 1321 times
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

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