Cheap Phase contrast part 2 : LOMO x20
Posted: Fri Apr 16, 2021 2:29 pm
Hello to you all,
I've received another Phase objective LOMO x20...and this time its the same series of my LOMO x40 that is a part of the LOMO phase contrast condenser KF-4.
And as i was disappointed with the x10 because i couldnt get nice images like I could with the x40 i was afraid ùy cheap technique might not work with the other objectives of the set.
And in fact this x20 works really fine !!! Even in critical illumination. I can use the same annulus I made for the x40 and located at the same place. All I have to do is to raise or lower the condenser until the size of the image of the annulus matches approx with the phase plate. (I use the phase telescope).
The image is much brighter and gives more colors depending how I raise the condenser. And its still proper phase contrast as i compared with my x40.
Here is a image extracted from a AVI I'll post on youtube. Its a vorticelli meeting another protozoa that was a bit too fast. The image will give you a rough idea of the brightness and contrast i could see..I'll post better pics soon..
Chris
I've received another Phase objective LOMO x20...and this time its the same series of my LOMO x40 that is a part of the LOMO phase contrast condenser KF-4.
And as i was disappointed with the x10 because i couldnt get nice images like I could with the x40 i was afraid ùy cheap technique might not work with the other objectives of the set.
And in fact this x20 works really fine !!! Even in critical illumination. I can use the same annulus I made for the x40 and located at the same place. All I have to do is to raise or lower the condenser until the size of the image of the annulus matches approx with the phase plate. (I use the phase telescope).
The image is much brighter and gives more colors depending how I raise the condenser. And its still proper phase contrast as i compared with my x40.
Here is a image extracted from a AVI I'll post on youtube. Its a vorticelli meeting another protozoa that was a bit too fast. The image will give you a rough idea of the brightness and contrast i could see..I'll post better pics soon..
- 16 avril 20 x20 essai 1.jpg (100.79 KiB) Viewed 1524 times