Interesting graphical description of the mathematical background of oblique lighting

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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MicroBob
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Interesting graphical description of the mathematical background of oblique lighting

#1 Post by MicroBob » Sun May 02, 2021 5:34 pm

Hi together,
Hubert has looked for an explanation of the relief effect of oblique lighting and created this interesting comparison of illumination techniques (in german language):
https://www.mikroskopie-forum.de/index. ... ;topicseen

Bob
Last edited by MicroBob on Mon May 03, 2021 1:08 am, edited 1 time in total.

tgss
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Location: Ontario, Canada

Re: Interesting gaphical description of the mathematical background of oblique lighting

#2 Post by tgss » Sun May 02, 2021 10:33 pm

Thank you for the link MicroBob. Looks very interesting. I shall work my way through it when I have a little more time.
Tom W.

Greg Howald
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Re: Interesting graphical description of the mathematical background of oblique lighting

#3 Post by Greg Howald » Mon May 03, 2021 1:30 pm

That is really good info. Thanks lots. Greg

Hobbyst46
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Re: Interesting graphical description of the mathematical background of oblique lighting

#4 Post by Hobbyst46 » Mon May 03, 2021 10:02 pm

Bob, It would be nice reading the full article, it there is an English version; the text is too long for DeepL sessions...
I would add that Oblique illumination is quite vivid in recent academic research, decorated with complex (pun) math equations...

MicroBob
Posts: 3154
Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: Interesting graphical description of the mathematical background of oblique lighting

#5 Post by MicroBob » Tue May 04, 2021 6:11 am

Hi together,
the limitation of oblique was always that it was not easy to get a repeatable effect. I can imagine that in a very modern microscope with stepper motor control of the oblique stop and digital imaging the resolution enhancing effect could be put to good use. Changeing the objective by means of a button press would then lead to a an automatic setting of condenser and stops. This could be a usefull contrast method for professional use.

Bob

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