PLAYING WITH OBLIQUE LIGHTING

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
Post Reply
Message
Author
Greg Howald
Posts: 1186
Joined: Tue Oct 20, 2020 6:44 am

PLAYING WITH OBLIQUE LIGHTING

#1 Post by Greg Howald » Wed May 26, 2021 1:51 pm

THESE TWO PHOTOS TAKEN WITH AMSCOPE T400, 20 WATT HALOGEN, USING OBLIQUE LIGHTING AND A DEGREE OF XPL. THOUGHT I'D LIKE TO SHARE.
GREG
Attachments
capsella embryo sec Green oblique (2).jpg
capsella embryo sec Green oblique (2).jpg (152.62 KiB) Viewed 1723 times
FLY MOUTH PARTS (2).jpg
FLY MOUTH PARTS (2).jpg (205.11 KiB) Viewed 1723 times
Top
User avatar
Rossf
Posts: 363
Joined: Mon Jan 20, 2020 12:39 am
Location: Victoria Australia

Re: PLAYING WITH OBLIQUE LIGHTING

#2 Post by Rossf » Fri May 28, 2021 11:42 pm

Nice images—Greg Howald-does XPL mean crossed polariser? Makes the fly mouth part look like darkfield. Oblique is so diverse and I suspect we haven’t seen the last of unique twists to it-I’m fanging to try VAC-need to buy some polarising sheets…
Keep it up
Ross
Top
Greg Howald
Posts: 1186
Joined: Tue Oct 20, 2020 6:44 am

Re: PLAYING WITH OBLIQUE LIGHTING

#3 Post by Greg Howald » Sat May 29, 2021 4:10 am

Yes. XPL means cross polarized light. Usually at 90 degrees, but I make attachments so I can rotate the polarizer to change the degree of polarization
Greg
Top
Post Reply

Return to “Illumination Techniques”