A question concerning resolution
Posted: Sat Jun 26, 2021 7:59 pm
We were all excited when we learned earlier in the month about a new lighting technique that allowed for higher resolution in light microscopy. I found that article most interesting.
Today I've been working on bright field at 100x. I will be using 100x for the next couple of months to run certain experiments. Don't tell OPEC. If they find out how much oil I'm using they'll raise the price!. ...
So while I was in the process of looking at several slides with oblique, polarized and phase contrast I took a look at a window film called Funfox. This material is in sheet form, very thin. Comes in a roll. Mirror surface on both sides but you get your eye close to it you can see through it. It's purpose is to reduce the amount of heat coming through your window on a bright and sunny day.
I took a small piece of this and examined it under the scope. It has a bit of a darkening effect, eliminates all glare, and is riddled with pin holes that seem random at first before you notice a repeating pattern.
I slid that thin slice of material under the slide on the scope. The image became even and clear. Not layered as much as usual. Not having to continually refocus.
I liked the result very much even though it dimmed the image slightly.
I wonder if anyone else has been dumb enough to do this, and I wonder if this result is an improvement in resolution. Comments appreciated. A lot of you folks are smarter and have more experience and knowledge than I will ever have and your input is cherished.
Greg
Today I've been working on bright field at 100x. I will be using 100x for the next couple of months to run certain experiments. Don't tell OPEC. If they find out how much oil I'm using they'll raise the price!. ...
So while I was in the process of looking at several slides with oblique, polarized and phase contrast I took a look at a window film called Funfox. This material is in sheet form, very thin. Comes in a roll. Mirror surface on both sides but you get your eye close to it you can see through it. It's purpose is to reduce the amount of heat coming through your window on a bright and sunny day.
I took a small piece of this and examined it under the scope. It has a bit of a darkening effect, eliminates all glare, and is riddled with pin holes that seem random at first before you notice a repeating pattern.
I slid that thin slice of material under the slide on the scope. The image became even and clear. Not layered as much as usual. Not having to continually refocus.
I liked the result very much even though it dimmed the image slightly.
I wonder if anyone else has been dumb enough to do this, and I wonder if this result is an improvement in resolution. Comments appreciated. A lot of you folks are smarter and have more experience and knowledge than I will ever have and your input is cherished.
Greg