Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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MichaelG.
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#1
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by MichaelG. » Sun Aug 08, 2021 8:44 am
Here’s an interesting little booklet, courtesy of the Biodiversity Heritage Library and the University of Leeds Library.
https://www.biodiversitylibrary.org/ite ... 1/mode/1up
ON AN EASY METHOD of VIEWING CERTAIN OF THE DIATOMACEAE.
MichaelG.
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Edit: corrected the library name
Last edited by
MichaelG. on Sun Aug 08, 2021 1:12 pm, edited 1 time in total.
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Hobbyst46
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#2
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by Hobbyst46 » Sun Aug 08, 2021 10:28 am
Thanks, Michael, for this fine combination of science and sweet prose !
Can we estimate its year of publication ?
How do we translate the article's terminology to modern terms ? like, what is today's equivalent of a 1/8 lens, who sells "spotted" lenses or objectives, etc ?
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Phill Brown
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#3
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by Phill Brown » Sun Aug 08, 2021 12:12 pm
I'll kick off with 1860's.
The author was 1816-1876?
I prefer dark field for diatoms, amazing how it was possible to resolve the pores of a diatom with the optics available.
I have a watchmakers eyeglass in a horn surround that gives an excellent image.
Likely to be far from new.
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BramHuntingNematodes
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#4
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by BramHuntingNematodes » Sun Aug 08, 2021 2:58 pm
Hobbyst46 wrote: ↑Sun Aug 08, 2021 10:28 am
Thanks, Michael, for this fine combination of science and sweet prose !
Can we estimate its year of publication ?
How do we translate the article's terminology to modern terms ? like, what is today's equivalent of a 1/8 lens, who sells "spotted" lenses or objectives, etc ?
An 1/8 lens has a magnification of 80x and it appears to have an NA of approximately 1.3 (1.56Xsin63 deg)
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination
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MichaelG.
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#5
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by MichaelG. » Sun Aug 08, 2021 6:05 pm
As I understand it … the Author’s “spotted” condenser lens was simply a hemisphere with a circular patch-stop.
Thus:
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MichaelG.
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P.S. 1/8 lens has a focal length of one eighth of an inch
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MichaelG.
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#6
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by MichaelG. » Sun Aug 08, 2021 6:23 pm
Too many 'projects'
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Hobbyst46
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#7
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by Hobbyst46 » Sun Aug 08, 2021 7:16 pm
Thanks.
So the spotted lens is a simple darkfield condenser ? and, when combined with the high NA objective, created oblique illumination ?
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MichaelG.
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#8
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by MichaelG. » Sun Aug 08, 2021 7:18 pm
Hobbyst46 wrote: ↑Sun Aug 08, 2021 7:16 pm
So the spotted lens is a simple darkfield condenser ?
Exactly that
MichaelG.
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MichaelG.
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#9
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by MichaelG. » Sun Aug 08, 2021 8:01 pm
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Here is what Conrad Beck was offering in 1921
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MichaelG.
Too many 'projects'