Dark Ground / Spotted Lens

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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MichaelG.
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Dark Ground / Spotted Lens

#1 Post by MichaelG. » Sun Aug 08, 2021 8:44 am

Here’s an interesting little booklet, courtesy of the Biodiversity Heritage Library and the University of Leeds Library.
https://www.biodiversitylibrary.org/ite ... 1/mode/1up
ON AN EASY METHOD of VIEWING CERTAIN OF THE DIATOMACEAE.
MichaelG.

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Edit: corrected the library name
Last edited by MichaelG. on Sun Aug 08, 2021 1:12 pm, edited 1 time in total.
Too many 'projects'

Hobbyst46
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Re: Dark Ground / Spotted Lens

#2 Post by Hobbyst46 » Sun Aug 08, 2021 10:28 am

Thanks, Michael, for this fine combination of science and sweet prose !
Can we estimate its year of publication ?

How do we translate the article's terminology to modern terms ? like, what is today's equivalent of a 1/8 lens, who sells "spotted" lenses or objectives, etc ?

Phill Brown
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Location: Devon UK.

Re: Dark Ground / Spotted Lens

#3 Post by Phill Brown » Sun Aug 08, 2021 12:12 pm

I'll kick off with 1860's.
The author was 1816-1876?
I prefer dark field for diatoms, amazing how it was possible to resolve the pores of a diatom with the optics available.
I have a watchmakers eyeglass in a horn surround that gives an excellent image.
Likely to be far from new.

BramHuntingNematodes
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Re: Dark Ground / Spotted Lens

#4 Post by BramHuntingNematodes » Sun Aug 08, 2021 2:58 pm

Hobbyst46 wrote:
Sun Aug 08, 2021 10:28 am
Thanks, Michael, for this fine combination of science and sweet prose !
Can we estimate its year of publication ?

How do we translate the article's terminology to modern terms ? like, what is today's equivalent of a 1/8 lens, who sells "spotted" lenses or objectives, etc ?
An 1/8 lens has a magnification of 80x and it appears to have an NA of approximately 1.3 (1.56Xsin63 deg)
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination

MichaelG.
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Location: North Wales

Re: Dark Ground / Spotted Lens

#5 Post by MichaelG. » Sun Aug 08, 2021 6:05 pm

As I understand it … the Author’s “spotted” condenser lens was simply a hemisphere with a circular patch-stop.

Thus:
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910E4A6D-96F4-4A47-A87E-75F92594A48C.jpeg
910E4A6D-96F4-4A47-A87E-75F92594A48C.jpeg (105.53 KiB) Viewed 3534 times
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MichaelG.
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P.S. 1/8 lens has a focal length of one eighth of an inch
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MichaelG.
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Re: Dark Ground / Spotted Lens

#6 Post by MichaelG. » Sun Aug 08, 2021 6:23 pm

Looks like the date was 1856 : https://journals.biologists.com/jcs/art ... m=fulltext

MichaelG.
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Hobbyst46
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Re: Dark Ground / Spotted Lens

#7 Post by Hobbyst46 » Sun Aug 08, 2021 7:16 pm

MichaelG. wrote:
Sun Aug 08, 2021 6:23 pm
Looks like the date was 1856 : https://journals.biologists.com/jcs/art ... m=fulltext

MichaelG.
Thanks.
So the spotted lens is a simple darkfield condenser ? and, when combined with the high NA objective, created oblique illumination ?

MichaelG.
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Re: Dark Ground / Spotted Lens

#8 Post by MichaelG. » Sun Aug 08, 2021 7:18 pm

Hobbyst46 wrote:
Sun Aug 08, 2021 7:16 pm
So the spotted lens is a simple darkfield condenser ?
Exactly that

MichaelG.
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MichaelG.
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Location: North Wales

Re: Dark Ground / Spotted Lens

#9 Post by MichaelG. » Sun Aug 08, 2021 8:01 pm

.

Here is what Conrad Beck was offering in 1921
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C8D17C44-3DFE-4EBD-BFC8-625FED02B146.jpeg
C8D17C44-3DFE-4EBD-BFC8-625FED02B146.jpeg (127.67 KiB) Viewed 3509 times
.

MichaelG.
Too many 'projects'

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