COL & phase contrast

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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smollerthings
Posts: 457
Joined: Thu Aug 19, 2021 12:10 pm

COL & phase contrast

#1 Post by smollerthings » Sat Oct 16, 2021 6:44 pm

Hi All,

I am a bit confused. I was playing with my phase contrast condenser and trying to achieve COL on my 40x (non phase contrast objective). COL is easy on the 10x but I couldn't quite get it on my 40x. And then I got this:



This looks like phase contrast but this objective does not have an annulus. What black magic is this?

Thanks!
Last edited by smollerthings on Sun Oct 17, 2021 7:18 am, edited 1 time in total.

BramHuntingNematodes
Posts: 1546
Joined: Tue Jan 21, 2020 1:29 am
Location: Georgia, USA

Re: COL & phase contrast

#2 Post by BramHuntingNematodes » Sat Oct 16, 2021 11:57 pm

I think it's col
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination

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