Four illuminations

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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TonyT
Posts: 184
Joined: Fri Sep 11, 2020 8:30 pm
Location: New Brunswick, CANADA

Four illuminations

#1 Post by TonyT » Mon Feb 27, 2023 4:01 pm

Canada Balsam mount of a Pseudoscorpion; not treated with KOH.
microscope images of a right leg to emphasize musculature
top left: brightfield,
top right: darkfield polarization,
bottom left: coloured polarization;
bottom right: DIC

Polarization clearly shows some of the muscles; DIC shows them all.
Attachments
pseudoscorpion 27ii23.jpg
pseudoscorpion 27ii23.jpg (100.44 KiB) Viewed 1436 times
pseudoscorpion 27ii23 plate.jpg
pseudoscorpion 27ii23 plate.jpg (171.83 KiB) Viewed 1436 times
New Brunswick
Canada

Sure Squintsalot
Posts: 393
Joined: Mon May 16, 2022 3:44 pm

Re: Four illuminations

#2 Post by Sure Squintsalot » Wed Mar 01, 2023 12:36 am

My copepods under DIC also reveal some hidden details:
Screenshot 2023-02-28 173150.jpg
Screenshot 2023-02-28 173150.jpg (109.91 KiB) Viewed 1343 times
It would seem that poor initial sample collection led to bacterial contamination and subsequent consuming of nearly all organic material rendering my copepods glass-clear. I'm assuming that the visible internal structure are probably interior pathways of organs, muscles, etc. Although I really do NOT know.

sreynolds
Posts: 113
Joined: Sat Oct 10, 2020 1:17 am
Location: Vermont, USA

Re: Four illuminations

#3 Post by sreynolds » Thu Mar 02, 2023 1:57 am

Tony - Can you please describe your equipment for the polarization images?
Steve

TonyT
Posts: 184
Joined: Fri Sep 11, 2020 8:30 pm
Location: New Brunswick, CANADA

Re: Four illuminations

#4 Post by TonyT » Thu Mar 02, 2023 12:37 pm

A "Simple Polarizing Attachment" on an Olympus BH2/BHS.
scope.jpg
scope.jpg (64.67 KiB) Viewed 1246 times
BH2-KP.jpg
Attachments
BH2-KP.jpg
BH2-KP.jpg (30.66 KiB) Viewed 1246 times
New Brunswick
Canada

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