Rhein berg illumination

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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paulg269
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Joined: Wed Apr 12, 2023 5:26 pm

Rhein berg illumination

#1 Post by paulg269 » Sat Jul 01, 2023 8:37 am

Hi , as Rheinberg is a derivative of dark field, does the size of the central stop matter as much? Ie do you need different sizes for each magnification. Thanks.

ADi
Posts: 306
Joined: Fri May 05, 2023 4:05 pm

Re: Rhein berg illumination

#2 Post by ADi » Sat Jul 01, 2023 2:07 pm

Hi,

Have you already visited this website?
https://www.olympus-lifescience.com/en/ ... rheinberg/

BTW, I often use Rheinberg filters to change the background of my photos.

Best, ADi

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josmann
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Re: Rhein berg illumination

#3 Post by josmann » Mon Jul 03, 2023 11:13 am

paulg269 wrote:
Sat Jul 01, 2023 8:37 am
Hi , as Rheinberg is a derivative of dark field, does the size of the central stop matter as much? Ie do you need different sizes for each magnification. Thanks.
I would say the stop size matters more for Reinberg. With dark field stops, there’s not a major issue if your central patch is oversized for the magnification you’re using. You’ll get less light than you could have - but that’s about it. With Reinberg, though, having an oversized central patch means that your oblique illumination will be contaminated by the light filtering through the central patch. You won’t get as nice of a contrast between the two colors you chose.
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MichaelG.
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Re: Rhein berg illumination

#4 Post by MichaelG. » Tue Jul 04, 2023 4:55 am

Another useful link : https://www.quekett.org/resources/rheinberg

Note: It’s dated 2013, so the software mentioned may be obsolete

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