Playing around with Polarized Light

Here you can discuss different microscopic techniques and illumination methods, such as Brightfield, Darkfield, Phase Contrast, DIC, Oblique illumination, etc.
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macnmotion
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Joined: Mon Sep 26, 2022 3:13 am

Playing around with Polarized Light

#1 Post by macnmotion » Wed Oct 25, 2023 8:05 am

Here are a few scenes from my Nikon inverted TMD Diaphot. 2 pieces of linear polarizing film and a full lambda wave plate.


PeteM
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Location: N. California

Re: Playing around with Polarized Light

#2 Post by PeteM » Wed Oct 25, 2023 10:44 pm

Nicely done - thanks for posting it.

Scoper
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Joined: Tue Jan 24, 2023 4:06 pm

Re: Playing around with Polarized Light

#3 Post by Scoper » Thu Oct 26, 2023 12:27 am

Brightfield only with the polarized light?

Why the waveplate?

Thanks for posting

macnmotion
Posts: 549
Joined: Mon Sep 26, 2022 3:13 am

Re: Playing around with Polarized Light

#4 Post by macnmotion » Thu Oct 26, 2023 4:28 am

Scoper wrote:
Thu Oct 26, 2023 12:27 am
Brightfield only with the polarized light?

Why the waveplate?

Thanks for posting
Yes this is brightfield. The order of pieces:

Lamp
Diffuser
Polarizing film
Wave plate
Slide
Polarizing film
Objective
Camera

The wave plate adds color into the background and otherwise non-birefringent portions of the subjects/material on the slide, making for a "prettier" results. Otherwise, the background is essentially dark due to the polarized light extinction. Rotating the wave plate (mine is shaped like a circular filter) changes the colors in the resulting image. I used a relatively inexpensive full lambda wave plate that I bought on eBay (about US$15).

Note: Please excuse any mis-use of terminology, I'm just learning this method of lighting.

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