Video: Paramecium: Anterior and Posterior CV; Cytostome Oral Ciliature

About the shape and function of different specimens
Post Reply
Message
Author
User avatar
linuxusr
Posts: 220
Joined: Sun Jun 24, 2018 9:00 pm
Location: Dominican Republic

Video: Paramecium: Anterior and Posterior CV; Cytostome Oral Ciliature

#1 Post by linuxusr » Wed Oct 13, 2021 8:09 pm

Axiocam 208 Color < LG 4K Monitor
200xTM
1.5% methylcellulose
timothy hay infusion

Definitions:

(1) Contractile Vacuole (CV): The CV is likely highly conserved in Paramecia, since without this organelle, the organism would lyse (rupture). The function is osmoregulation, maintaining equilibrium between solutions external to the organism and within the cytoplasm of the organism. Paramecia maintain two CV's, one anterior and one posterior--by my observation on the dorsal side--opposite the cytostome (“mouth”) on the ventral side.
(2) cytostome: elliptical oral opening through which food enters to the endoplasm, in this specimen, along a long axis. In Video D, you can see the wavelike motion of the oral ciliature (feeding function), whereas in Video C, you get get a view of the ciliary girdle (locomotive function).

**This media fails to embed. Sorry.
Chart from Google Images: [media] https://imgur.com/a/zzYpIe0 [/media]

More information here from Wikipedia:

https://en.wikipedia.org/wiki/Contractile_vacuole


Video A: https://youtu.be/lRfI2wfJ3lk
To see both functioning CV’s, look first to the posterior (bottom) end, them immediately to the anterior end (up). If you faintly see some cytostome cilia, you are looking through the organism to the dorsal side. When the specimen rotates, it will be obvious that you are now viewing the cytostome directly. From this observation, I inferred that this is the ventral side. **It’s very possible that I have flipped ventral/dorsal.

Video B: https://youtu.be/gNdwlpE7rLM
In this very short video, on the first rotation of the specimen, you will see both organelles at the same point in the diastole/systole cycle.
(H20 < CV = diastole; CV < H20 = systole)

Video C: https://youtu.be/baraq65iBCk
A variation with a good view of the ciliary girdle.

Video D: https://youtu.be/A6VUJBW_Z8A
This video gives a good view of the oral ciliature. Since the organism rotates 360 degrees, you are looking at the same side.
Nikon AlphaPhot 2 < Zeiss Primostar 3, Full Köhler; Axiocam 208 Color < UHD LG
Aller Anfang ist schwer.

Post Reply