New member and new to microscopy

What is your microscopy history? What are your interests? What equipment do you use?
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Pronin
Posts: 1
Joined: Wed May 03, 2023 1:36 am

New member and new to microscopy

#1 Post by Pronin » Thu May 04, 2023 10:54 pm

Hi to all, and thanks for the acceptance in this forum.

I'm kinda a handy-man and have several hobbies, mostly focused on metalworking.
At some point I bougth a Amscope stereo microscope for working on intrincate small mechanism and inspection, and despite the cheap price, looking intro the eyepieces at anythig was WOW!, and I have been looking at everithing on hand from then on (insecs, dirt, plants, etc...), also I got very interested in optical measure of length.

Now, I got to a antique store and see a dusty and forgotten light microscope, I recognice the brand Carl Zeiss Jena (as I own a couple of metrology instruments from that brand, and they are good) and got it dirt cheap. The microscope is complete, but full of dust and rust, all optics are fogged and dusty, and the electrical parts have been intervened and destroyed.

After some research, the microscope is a Laboval 2, seems a routine microscope for the 70´s. I have not been able to find the owner manual or service manual, or any information at all exept for some mechanical dissasembly of the fine focus system.

It came with all original Carl Zeiss Jena parts:

- two A10X 14 Eyepieces

Objectives:
- 3.2 / 0.10 Semiplan
- 10 / 0.25
- 40 / 0,65
- HI 100 / 1,25 oil inmersion with a iris??

Also it has a 1,6X binoviewer. It seems that with the 40x objective the magnification (40 x 1,6 x 10 = 640 ??) its almost getting intro empy magnification treshold?? the same with the 100X. not sure :?:

For a couple of weeks I have been working on it, cleaning and bringing it to fully mechanical function (never have seen grease goin so hard!) and also optical cleaniness. Now I can use it but the lamp housing and electrics still need some work.

Using the cellphone flashlight on top of the base for ilumination, I put a drop of a rain water pond on top of a slide and I was able to see very small dots revoling on it, very impresive! pushing the magnification I see these are microorganism with I could not focus properly because of the very shallow deep of field :?: , or maybe still fogged and dusty optics (inside the binoviewer witch I haven't been able to disassembly), or the very poor ligth source and optical misligment of components after all the disassembly, I dont now, but I hope to solve it!

Many thans in advance for your advise and teachings.
Best Regards.

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imkap
Posts: 754
Joined: Thu Nov 25, 2021 9:44 pm

Re: New member and new to microscopy

#2 Post by imkap » Thu May 04, 2023 11:45 pm

Hi and welcome. Congratulations on your purchase, microscopes are fun to take apart... :D

Depth of field is shallow on large magnification, it is how it is... :D You can put a cover glass to limit the vertical movement of the specimen...

Turning/moving different parts in the optical path tells you which part has the visible dirt on. If the dirt isn't visible on the image than it just reduces contrast, which may or may not be worth a hassle/risk of cleaning and disassembly of a part, depending on how much contrast deterioration (dirt) is there. Bino heads can be frustrating...

I like how that microscope looks, very utilitarian, from a field hospital.

PeteM
Posts: 3013
Joined: Tue Dec 06, 2016 6:22 am
Location: N. California

Re: New member and new to microscopy

#3 Post by PeteM » Fri May 05, 2023 12:01 am

Specimens need to be very thin at high magnification. Putting a coverslip on top of that drop of water, if you didn't already have one, will help. Higher magnification biological objectives will also want to see that thin .17mm bit of glass on top of the specimens.

A proper light source will also help a lot. The only Laboval I have is slightly different, a "4" I believe. It has a focusing condenser and, surprisingly, a still good power supply. If you have a focusing condenser under your stage, with an iris, then getting a fairly bright and fairly well-collimated beam of light is all you need. A mirror aimed at the Sun will work. Something like a LED headlamp or a LED photo lamp could work. Here's one option: https://www.amazon.com/gp/product/B07YS ... UTF8&psc=1

Better if it actually had focusing (e.g. LED headlight or bike light). Still better if you could place an iris directly above to control the light a bit better.

The East Germans were still in "Bauhaus" design mode when yours was built. It's a simple but elegant scope.

Welcome aboard.

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