I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

Everything relating to microscopy hardware: Objectives, eyepieces, lamps and more.
Message
Author
viktor j nilsson
Posts: 760
Joined: Thu Aug 23, 2018 10:12 pm
Location: Lund, Sweden

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#31 Post by viktor j nilsson » Wed Jun 30, 2021 3:46 pm

That is very impressive, apo. And I, too, am very happy for Seta. Looking forward to seeing the results!

Scarodactyl
Posts: 2775
Joined: Sat Mar 03, 2018 9:09 pm

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#32 Post by Scarodactyl » Wed Jun 30, 2021 6:33 pm

Yeah, let me second that, I am very impressed.

Chris Dee
Posts: 216
Joined: Mon Aug 12, 2019 1:02 pm

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#33 Post by Chris Dee » Thu Jul 01, 2021 4:35 pm

Well done apochronaut, no easy task.

hans
Posts: 987
Joined: Thu May 28, 2020 11:10 pm
Location: Southern California

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#34 Post by hans » Fri Jul 02, 2021 11:14 pm

Yeah, very impressive. Meanwhile I managed to accidentally spill out the contents of a working Leica 10X phase objective. Those warnings you see about carefully marking and preserving rotations of all the groups are not exaggerated! Here is a photo showing the extreme coma and astigmatism in the center of the field after random reassambly. (Subject is dilute milk using 100X phase annulus for darkfield.) Apochromats must be even more sensitive. Fortunately this is a plan achro objective (originally from an ATC2000 student-oriented microscope, I think) that I paid $20 for so not too expensive a lesson.
Attachments
P1080176.jpg
P1080176.jpg (100.65 KiB) Viewed 3668 times

apochronaut
Posts: 6272
Joined: Fri May 15, 2015 12:15 am

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#35 Post by apochronaut » Mon Jul 05, 2021 11:33 am

That looks like it might be in the rotation of the second cell but that is pretty bad. Those come apart pretty easy. Usually there are random vertical scratches along the sides of the cells. Sometimes , you can match them up between cells and get a cell to cell alignment. If you are really lucky , you can sometimes find the complementary are on the barrel that caused the scratches and get a cell to barrel alignment. Unfortunately, there is no easy way of getting the shim order and location correct, once shuffled. Usually, if the shims are jumbled up, you get spherical aberration and the parfocality shifts.

In your example above, try fiddling with the diaphragm/annulus relativity. Some of that image skewing might get taken up with a DF centering adjustment.

User avatar
patta
Posts: 402
Joined: Sun May 10, 2020 6:01 am
Location: Stavanger Norway
Contact:

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#36 Post by patta » Mon Jul 05, 2021 12:23 pm

hans wrote:
Fri Jul 02, 2021 11:14 pm
Here is a photo showing the extreme coma and astigmatism in the center of the field after random reassambly. (Subject is dilute milk using 100X phase annulus for darkfield.)
The coma may be fixable: remove the skirt; loosen a bit the screw that holds the lens pack. Toward the end of the objective, there are usually four little holes. With the objective mounted, observing the milk, fiddle a bit inside those holes, like with a toothpick; there is a loose lens inside that can fix collimation. With some luck, it should get better. Some objectives don't have those holes though.

http://www.funsci.it/files/obbiettivi.pdf
(Sorry it is a pdf, and in italian. see illustration at pag. 56 for example)

apochronaut
Posts: 6272
Joined: Fri May 15, 2015 12:15 am

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#37 Post by apochronaut » Mon Jul 05, 2021 12:30 pm

patta wrote:
Mon Jul 05, 2021 12:23 pm
hans wrote:
Fri Jul 02, 2021 11:14 pm
Here is a photo showing the extreme coma and astigmatism in the center of the field after random reassambly. (Subject is dilute milk using 100X phase annulus for darkfield.)
The coma may be fixable: remove the skirt; loosen a bit the screw that holds the lens pack. Toward the end of the objective, there are usually four little holes. With the objective mounted, observing the milk, fiddle a bit inside those holes, like with a toothpick; there is a loose lens inside that can fix collimation. With some luck, it should get better. Some objectives don't have those holes though.

http://www.funsci.it/files/obbiettivi.pdf
(pdf, in italian sorry. see illustration at pag. 56 for example)
Not with this objective design.

hans
Posts: 987
Joined: Thu May 28, 2020 11:10 pm
Location: Southern California

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#38 Post by hans » Mon Jul 05, 2021 9:05 pm


viktor j nilsson
Posts: 760
Joined: Thu Aug 23, 2018 10:12 pm
Location: Lund, Sweden

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#39 Post by viktor j nilsson » Mon Jul 19, 2021 10:45 pm

My £30 Nikon CFN PlanApo 100x 1.40 arrived today, and it is in much better shape than I could ever have hoped for. The front lens is pristine, and I can't see any defects internally besides a little dust on the rear element. A quick test with a calibration slide revealed a very sharp, contrasty image. So I won't need to do any assembling on this one.

LouiseScot
Posts: 1167
Joined: Tue Jul 07, 2020 1:51 pm
Location: Scotland

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#40 Post by LouiseScot » Mon Jul 19, 2021 10:51 pm

viktor j nilsson wrote:
Mon Jul 19, 2021 10:45 pm
My £30 Nikon CFN PlanApo 100x 1.40 arrived today, and it is in much better shape than I could ever have hoped for. The front lens is pristine, and I can't see any defects internally besides a little dust on the rear element. A quick test with a calibration slide revealed a very sharp, contrasty image. So I won't need to do any assembling on this one.
That was remarkably cheap!
Enjoy!

Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo

Seta
Posts: 131
Joined: Thu Feb 18, 2021 8:24 pm
Location: Castellón, Spain

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#41 Post by Seta » Thu Sep 02, 2021 12:59 pm

viktor j nilsson wrote:
Mon Jul 19, 2021 10:45 pm
My £30 Nikon CFN PlanApo 100x 1.40 arrived today, and it is in much better shape than I could ever have hoped for. The front lens is pristine, and I can't see any defects internally besides a little dust on the rear element. A quick test with a calibration slide revealed a very sharp, contrasty image. So I won't need to do any assembling on this one.
Congrats!! that does not happen very often.

After some delays due to Spanish customs I finally got the lens back from Apochronaut; it looked very good but I still needed to test it.

I run a quick test on a mosquito slide against the Nikon CF APO 40 1.0 oil; I do not know how a new, factory calibrated Nikon CFN 60/1.40 Oil does perform but this one is very good, Apochronaut did an excellent job at fixing it.

These are 100% crops from a test with nikon V1. First crop shows the 60X as it came out, then the 60x with CAs auto fixed in camera raw and then the 40X with CAs auto fixed too. Truth is the CA correction on the 40X shot has no effect or very mild, do not ask me why.

Resolving power looks pretty similar but my only BH2 condenser for now is the flip out NA0.95 one, and the iris was a little closed too. I still have to receive some stuff for the scope but I am going abroad for a couple of months..I guess it will be complete by December or so.

Image

Full size

Another test shot, this time also including the CFN 100/1.25 DIC Oil I own; some levels applied.

Image

Full size

To my untrained eyes the CFN 60/1.40 is better corrected than the 40X but the 40X may (and only maybe) have a little better contrast.

apochronaut
Posts: 6272
Joined: Fri May 15, 2015 12:15 am

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#42 Post by apochronaut » Thu Sep 02, 2021 2:33 pm

I am very glad that it works so well for you. It seems a fairly common circumstance for 60X objectives to have lower contrast than 40X objectives from the same series. At least in any pairings that I have been able to compare but maybe not so much with oil immersion objectives of high N.A.
It serms to be magnification to N.A. ratio dependent . A typical 60X .85 dry objective has a 70:1 ratio of magnification to N.A.. A 40X .65 in the same series has a ratio of 61.5. A higher ratio will give lower contrast with all other factors being equal.
Your 60X and 40X apos are 42 and 40 respectively, so not much difference.

Seta
Posts: 131
Joined: Thu Feb 18, 2021 8:24 pm
Location: Castellón, Spain

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

#43 Post by Seta » Thu Sep 02, 2021 3:16 pm

apochronaut wrote:
Thu Sep 02, 2021 2:33 pm
It serms to be magnification to N.A. ratio dependent .
Interesting way of seeing it, never though of it like that.

I will try to run a focus stack with both lenses before I leave but I am going to be quite busy the next few days.
I am more interested in final image results than just how single frames look

Post Reply