Leica DM IRBE Inverted
Leica DM IRBE Inverted
I recently picked up a pretty complete Leica DM IRBE Inverted scope. I know very little about the scope itself. I haven't even used it yet and am waiting for a bulb. Does anyone have direct experience with this scope? I was also wondering IF software is obtainable for this particular scope? Someone gave me this link but they didn't know if it was compatible with the Leica DMIRBE.
https://micro-manager.org/wiki/LeicaDMI
Regards,
-JW:
https://micro-manager.org/wiki/LeicaDMI
Regards,
-JW:
- Attachments
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- 20210518_161440.jpg (195.69 KiB) Viewed 3123 times
Re: Leica DM IRBE Inverted
Just an update just in case someone might have operational experience with this particular microscope. I've seen a few Youtube videos where the seller has it operational. I've acquired a few more parts.
https://pbase.com/smokedaddy/image/171761490
and the rest are here.
https://pbase.com/smokedaddy/leica_dm_irbe_parts
-JW:
https://pbase.com/smokedaddy/image/171761490
and the rest are here.
https://pbase.com/smokedaddy/leica_dm_irbe_parts
-JW:
Re: Leica DM IRBE Inverted
After a period of 13 months of persistent effort, involving consultations with multiple microscope forums, individuals, Leica themselves, as well as microscope dealers and repairmen, I have successfully achieved the functioning of the Leica DMIRB/E with MicroManager. This achievement allows for the microscope to be software-controlled, replacing the need for manual operation, which was previously unattainable. While there remains additional work to be done, I am determined to persist in my efforts. It is worth noting that this accomplishment may be of limited interest, as only a small group of individuals possess the requisite knowledge to appreciate its significance. Nevertheless, I am proud of the results achieved through my efforts. It should be noted that in its original configuration, this particular microscope had a substantial cost of approximately 400K and required a team of engineers to operate in its confocal/laser setup, whereas I am utilizing it in its simplest form as a stand-alone microscope.
The Leica DMIRBE confocal microscope was a research-grade instrument designed for high-resolution imaging of biological samples. Confocal microscopy is a specialized imaging technique that uses a laser to illuminate a single point on a specimen, allowing for the collection of thin, optical sections from within a sample. These optical sections can be used to reconstruct a three-dimensional image of the specimen, allowing for detailed analysis of its structure and function. The Leica DMIRBE was particularly useful for imaging thick, multi-layered samples, such as tissue sections or living organisms, and was used in a variety of applications, including developmental biology, neurobiology, cell biology, and cancer research. Its ability to generate high-quality images with exceptional clarity and resolution made it a valuable tool for researchers studying the complex structures and functions of living organisms at the cellular level.
I realize the image is not spectacular as I am still in the learning stages and used the incorrect prisms for the objective being used.
-JW:
The Leica DMIRBE confocal microscope was a research-grade instrument designed for high-resolution imaging of biological samples. Confocal microscopy is a specialized imaging technique that uses a laser to illuminate a single point on a specimen, allowing for the collection of thin, optical sections from within a sample. These optical sections can be used to reconstruct a three-dimensional image of the specimen, allowing for detailed analysis of its structure and function. The Leica DMIRBE was particularly useful for imaging thick, multi-layered samples, such as tissue sections or living organisms, and was used in a variety of applications, including developmental biology, neurobiology, cell biology, and cancer research. Its ability to generate high-quality images with exceptional clarity and resolution made it a valuable tool for researchers studying the complex structures and functions of living organisms at the cellular level.
I realize the image is not spectacular as I am still in the learning stages and used the incorrect prisms for the objective being used.
-JW:
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- Focus working.PNG (126.22 KiB) Viewed 2208 times
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- 4-FB-PS-2023-02-25-03.04.jpg (169.66 KiB) Viewed 2208 times
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- Leica-DMIRBE-setup.jpg (91.71 KiB) Viewed 2208 times
Last edited by JWW on Mon Feb 27, 2023 11:38 pm, edited 2 times in total.
Re: Leica DM IRBE Inverted
Congratulations on getting it running. Mechanical systems submit fairly readily to discovery and tinkering. No so with microscopes missing their software and its documentation.
Re: Leica DM IRBE Inverted
Thanks PeteM,
Not a big deal to most, but a massive ordeal for me.
-JW:
Not a big deal to most, but a massive ordeal for me.
-JW:
Re: Leica DM IRBE Inverted
So you got the laser part working too? Wow, Lord Vader, at this speed, you are going to build yourself another Death Star very soon…….it is an understatement to say that I have goosebumps from reading that.
Re: Leica DM IRBE Inverted
Absolutely NOT. The lasers were toast. I thought I clarified that in my posting. There are a plethora of other components to make this function as a confocal scope. This is simply working as a standalone scope controlled by software, that being Micromanager. There are no indicator markets on the focus knob, it's an encoder. The turret is motorized as well. I simply have MM making it functional as a standalone microscope. If I get the Z-Glavo stage working in MM, then that's another accomplishment. I am a novice, not a microscope engineer.
Thanks for thinking that I might have Vader skills,
-JW:
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- Location: Devon UK.
Re: Leica DM IRBE Inverted
That's a project I would not have taken on, sure you will get further a step at a time.
Out there somewhere are the parts you need.
I replaced a laser light pin on at the time 20 year old CD player (Phillips/Boothroyd) that was said couldn't be done,only to find it was a faulty capacitor, lesson was shouldn't have just accepted the original diagnosis from symptoms only.
Out there somewhere are the parts you need.
I replaced a laser light pin on at the time 20 year old CD player (Phillips/Boothroyd) that was said couldn't be done,only to find it was a faulty capacitor, lesson was shouldn't have just accepted the original diagnosis from symptoms only.