Color saturation in videos

[Javier]

Hi,

I recorded the attached video of a Bdelloid Rotifer yesterday, and despite I don't expect to get high-quality videos with my current equipment, I wonder why the color saturation is that low compared to most of the microscopy works I see. Is it because of the afocal technique, the quality of my optics, or something related to my microscopy or post-processing skills?

Thanks in advance,
Javier.

[Javier]

I tried playing with the saturation of another video from the same sample. Would you say this is better? Or it would be somehow artificial?

[BramHuntingNematodes]

well how does the image compare to what you see through the eyepiece?

[Javier]

I think I see something between the two videos through the eyepiece. Hard to tell, though...

[BramHuntingNematodes]

The second video does not appear to be oversaturated from my experience. My problems with cameras are usually with hue, and I check very frequently against the eyepiece view.

[Javier]

And do you adjust your hue accordingly?

[BramHuntingNematodes]

Definitely. I am paranoid about the LED lighting I use having strange effects on the camera. Anyway, I calibrate every time I open the software I use.