Full servicing of a NACHET NS400

Everything relating to microscopy hardware: Objectives, eyepieces, lamps and more.
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Hobbyst46
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Re: Full servicing of a NACHET NS400

#31 Post by Hobbyst46 » Wed Jan 12, 2022 11:33 am

BramHuntingNematodes wrote:
Wed Jan 12, 2022 1:57 am
Congrats, it's beautiful
+1

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mrsonchus
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Location: Cumbria, UK

Re: Full servicing of a NACHET NS400

#32 Post by mrsonchus » Wed Jan 12, 2022 11:54 am

Really nicely-done! I've been following along this interesting thread and must say your 'scope looks mighty-fine in that last image.
Looks like you've most certainly achieved and of course learnt an awful lot. Very interesting and very well done.
John B

ker2x
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Re: Full servicing of a NACHET NS400

#33 Post by ker2x » Fri Jan 21, 2022 7:32 pm

mrsonchus wrote:
Wed Jan 12, 2022 11:54 am
Really nicely-done! I've been following along this interesting thread and must say your 'scope looks mighty-fine in that last image.
Looks like you've most certainly achieved and of course learnt an awful lot. Very interesting and very well done.
haha thank you, but I'm not done yet :)

I bought a 3D Printer, a Kingroon KP3S, excellent quality/price ratio, small, cute, but large enough for my need (it print 18x18x18cm max).

I'll still planning to use that busted Trinocular port and I'm experimenting various mount and technique to make the phototube.

and also making toy for fun

Image

Scarodactyl
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Re: Full servicing of a NACHET NS400

#34 Post by Scarodactyl » Fri Jan 21, 2022 7:33 pm

A 3d printer is the ultimate tool for making camera to microacope adaptera. This will make it easy.

ker2x
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Re: Full servicing of a NACHET NS400

#35 Post by ker2x » Mon Jan 24, 2022 11:40 am

Prototype in progress (wrong tube length to save printing time)

Image

dtsh
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Location: Wisconsin

Re: Full servicing of a NACHET NS400

#36 Post by dtsh » Mon Jan 24, 2022 3:24 pm

Looks good, I have something similar I put on my Microstar IV for imaging. 3D printing is so handy.

ker2x
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Re: Full servicing of a NACHET NS400

#37 Post by ker2x » Fri Jan 28, 2022 2:24 am

I sent back my bench power supply, twice. Ordered a 3rd, from a different brand this time.
This adventure begin to look like a romance light novel with plot twist and cliffhanger at every chapters :roll:

3rd time's a charm ?

I also begun working on designing a new lighthouse in Fusion 360.

ker2x
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Re: Full servicing of a NACHET NS400

#38 Post by ker2x » Fri Jan 28, 2022 3:22 am

Still a work in progress as I have to learn fusion360 while designing

Image

ker2x
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Re: Full servicing of a NACHET NS400

#39 Post by ker2x » Sat Jan 29, 2022 12:47 pm

Image

I gave up on the idea of a full length phototube :
- pro : higher field of view for my tiny sensor
- con : it's out of focus, I can't have a clear view of the sample on both the ocular and camera at the same time.

I used a M35 threading and it fit nicely-ish enough. (it doesn't look like the thread truly is M35, it seems to have non-metric pitch)
(And yes, surprisingly enough, even a low cost 3d printer is able to print thread. I also printed a c-mount without much trouble)

I'll probably build an extender. Considering the cost of making this, it doesn't matter much.

It used 17g of PLA (0.41euro) and printed in 1h37mn.
If you count labor cost, and excluding anything else like shipping, R&D, the cost of the printer, etc : that would make it a 25 euro piece.
Yes, PLA is very cheap. :D (I count 25e/kg but you can get some for less)

The highest cost would come from the R&D labor cost, I have a nice box of discarded (not failed) tube & test as I am learning how to design (CAD) and print.
I truly failed only 1 print so far but it was a mistake on my side. (google "3d printer spaghetti")

PS : the tube is rigid and strong, it would require great effort to break it by hand, and I only used a weak 10% infill (saving on printing time). with a 100% infill it would be impossible to break without tool

Scarodactyl
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Re: Full servicing of a NACHET NS400

#40 Post by Scarodactyl » Sat Jan 29, 2022 6:55 pm

Veey nice! Going to have some pics to share soon?
For a longer term part you might want to consider petg. Pla is strong enough but over lkng periods can flow under pressure.

ker2x
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Re: Full servicing of a NACHET NS400

#41 Post by ker2x » Sat Jan 29, 2022 7:45 pm

Scarodactyl wrote:
Sat Jan 29, 2022 6:55 pm
Veey nice! Going to have some pics to share soon?
For a longer term part you might want to consider petg. Pla is strong enough but over lkng periods can flow under pressure.
tomorrow, after testing the dark field filter :)

ho yeah i forgot to mention that I received my new new new PSU today as the 2 other one where defective.
So I changed brand and got a Eventek 0-30V 0-10A DC

ker2x
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Re: Full servicing of a NACHET NS400

#42 Post by ker2x » Sat Jan 29, 2022 9:00 pm

A quick video, the fps drop is caused by the computer I used. I recycled one of my old laptop but it can't handle OBS apparently.
will use a proper one next time.

I have a few issue to fix. Not sure why the sample appear to move when I refocus. I'm pretty sure it didn't do that before so I might even messed up something somewhere.

The video show bright field, and polarized light.
I tried dark field (not in this video) but I have no idea where to put the filter because of the weird configuration of the microscope.
There is one place I didn't try yet, but it's the worst place for me (and probably the exact right place for this microscope) I'll explain later.

The video :

ker2x
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Re: Full servicing of a NACHET NS400

#43 Post by ker2x » Sat Jan 29, 2022 9:11 pm

About dark field, I checked the manual, and of course it's at the worst place.

This microscope have tons of module, for dark field, phase contrast, interference contrast, fluorescence, even laser ...
You can insert module on top of the microscope (eg : fluorescence, reflected light), or below (eg : dark field, pass contrast)

The module on the low side insert like a cartridge from the side.
OF COURSE, I don't have any of this.

(top : without module, bottom : with module)
Image

So I have to build the cartridge-like module, without any plan or idea or what it's supposed to look like.

it even have an additional top module for dark field with high power oil immersion objective

Image

ker2x
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Re: Full servicing of a NACHET NS400

#44 Post by ker2x » Sun Jan 30, 2022 5:51 pm

so this is where I should have my dark field filter :shock:

Image

Image

ker2x
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Re: Full servicing of a NACHET NS400

#45 Post by ker2x » Sun Jan 30, 2022 6:04 pm

I need to build some kind of adjustable filter holder so I don't have to reprint the whole plate every time.
I hope you can tell just from the picture that it's going to be a massive PITA, because it is.

viktor j nilsson
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Re: Full servicing of a NACHET NS400

#46 Post by viktor j nilsson » Sun Jan 30, 2022 6:19 pm

That place seems like an elegant place to put filters if you are a producer that wants to make nice, ready made filter inserts.

But are you sure that it is really the only place you can place those filters?

The requirement is that a darkfield stop should be placed at a plane that is conjugate with the front focal plane of the condenser (which, in turn, is conjugate to the back focal plane of the objective). Your location under the microscope is maybe one plane that is conjugate with the front focal plane of the condenser. But I don't see why you couldn't place your darkfield filter at the actual front focal plane of the condenser, i.e. as close to the condenser aperture, where most DIY users tends to place them.

Have you tried placing something (like a needle) just below your condenser and looked through a phase telescope to see if you can see the needle sharply in focus at the objective back focal plane?

ker2x
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Re: Full servicing of a NACHET NS400

#47 Post by ker2x » Sun Jan 30, 2022 6:35 pm

viktor j nilsson wrote:
Sun Jan 30, 2022 6:19 pm
That place seems like an elegant place to put filters if you are a producer that wants to make nice, ready made filter inserts.

But are you sure that it is really the only place you can place those filters?

The requirement is that a darkfield stop should be placed at a plane that is conjugate with the front focal plane of the condenser (which, in turn, is conjugate to the back focal plane of the objective). Your location under the microscope is maybe one plane that is conjugate with the front focal plane of the condenser. But I don't see why you couldn't place your darkfield filter at the actual front focal plane of the condenser, i.e. as close to the condenser aperture, where most DIY users tends to place them.

Have you tried placing something (like a needle) just below your condenser and looked through a phase telescope to see if you can see the needle sharply in focus at the objective back focal plane?
Well, I tried various place,and I do have a Bertrand lens on this microscope.
The thing is, as odd as it may look like, the diaphragm you see on the picture IS the aperture diaphragm :(
When I do the focus with the Bertrand lens, this is the one I see.

The place you see on the picture is between the field diaphragm and the aperture diaphragm.

I have a filter holder between the aperture diaphragm and the lighthouse (with ND/IR/Blue filter). I tried putting a dark field filter here without much success.
I can try again, perhaps I did something wrong

ker2x
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Re: Full servicing of a NACHET NS400

#48 Post by ker2x » Sun Jan 30, 2022 7:06 pm

Also, I need to translate what you said in beginner vocabulary.

There is an image of my sample :
- in my eye
- somewhere between the eyepiece and the sample
- at the sample itself
- between the sample and the light source.

correct ?

Same for the light source.

And these image are alternating in the light path
light -> sample -> light -> sample -> light -> sample

And the dark field filter need to be where there is a virtual image of the sample, correct ?

And one of this place Is between the two lens at the bottom of the image where the 2 line of the light are parallel (which mean there would be an image of the sample here).
or am I totally wrong and I didn't understand anything about how microscope works ? :D

Image

ker2x
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Re: Full servicing of a NACHET NS400

#49 Post by ker2x » Sun Jan 30, 2022 7:09 pm

And from what you tell me there should also be a image of the sample inside the condenser ?

PS : this diagram is the diagram of my microscope, from the manual. Except that I don't have the lighthouse at the top and the module for reflected light with the semi-mirror (don't know the real name) at 45°

ker2x
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Re: Full servicing of a NACHET NS400

#50 Post by ker2x » Sun Jan 30, 2022 7:15 pm

So the filter could be at any of the 2 place shown with a red line ?

Image

ker2x
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Re: Full servicing of a NACHET NS400

#51 Post by ker2x » Mon Jan 31, 2022 4:51 pm

I tried inside the condenser. all it does is block the light ...

ker2x
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Re: Full servicing of a NACHET NS400

#52 Post by ker2x » Mon Jan 31, 2022 11:28 pm

I got help from discord, found the right alternative position. building a filter holder tomorrow :)

ker2x
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Re: Full servicing of a NACHET NS400

#53 Post by ker2x » Tue Feb 01, 2022 10:42 am

First prototype, I played with a mesh design tube, the final result will be full solid (or not) :
Image

ker2x
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Re: Full servicing of a NACHET NS400

#54 Post by ker2x » Sat Feb 05, 2022 9:02 am

I built more and more and better filter holder and filters. I have a useable dark field for 1 objective for now :)

ker2x
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Re: Full servicing of a NACHET NS400

#55 Post by ker2x » Tue Feb 08, 2022 12:24 am

Image
Image

ker2x
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Re: Full servicing of a NACHET NS400

#56 Post by ker2x » Tue Feb 08, 2022 12:29 am

I still have a long way to go, but I'm really enjoying this :mrgreen:

Scarodactyl
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Re: Full servicing of a NACHET NS400

#57 Post by Scarodactyl » Tue Feb 08, 2022 12:29 am

That is a really neat design, well done!

ker2x
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Re: Full servicing of a NACHET NS400

#58 Post by ker2x » Tue Feb 08, 2022 5:57 pm

The printing failed (incorrect support parameter) but the size is right.

Image

ker2x
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Re: Full servicing of a NACHET NS400

#59 Post by ker2x » Sun Feb 13, 2022 12:44 pm

by the way : I just bought this, I should receive it in a week or two.
With dark field condenser, and a working PSU.

Image

ker2x
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Re: Full servicing of a NACHET NS400

#60 Post by ker2x » Mon Feb 21, 2022 9:05 pm

I finished printing this today

Image

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