Replace filters in Zeiss Standard Epi-Fluorescence filter cube

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DevonianSea
Posts: 11
Joined: Sun Aug 22, 2021 1:07 am

Replace filters in Zeiss Standard Epi-Fluorescence filter cube

#1 Post by DevonianSea » Mon Dec 27, 2021 9:10 pm

Hi all,
I've just got myself the Zeiss Epi-Fluorescence condenser (46 60 00) with a HBO50 (mercury arc) and a filter cube (46 63 01-9901). The filter cube contains two sets of filters, I don't know which exactly, but those are near-useless to me, as I'm specifically interested in 365nm UV fluorescence.

Now, is there a way to change the filters in the filter cube to allow UV-epi-fluorescence, and if so, how would one do that?
Last edited by DevonianSea on Tue Dec 28, 2021 9:52 am, edited 1 time in total.

crb5
Posts: 101
Joined: Sat Sep 12, 2020 11:55 pm

Re: Replace filters in Zeiss Epi-Fluorescence filter cube for Standard

#2 Post by crb5 » Tue Dec 28, 2021 2:06 am

You might find some useful advice on the Semrock website https://www.semrock.com/cube-assembly-instructions.aspx. They also cover such things at getting the orientation of the dichroic beam splitter right.

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