Npl fluotar 40L 0.60
Npl fluotar 40L 0.60
Cordial greetings.
Does anyone have a solution for using this objective on a non-inverted microscope?
Does anyone have a solution for using this objective on a non-inverted microscope?
- Attachments
-
- IMG_20220124_213929.jpg (72.67 KiB) Viewed 2236 times
-
- Posts: 6327
- Joined: Fri May 15, 2015 12:15 am
Re: Npl fluotar 40L 0.60
Use a measured slide as a coverslip. There are also thick coverslips used for haemocytometers , usually .4 but I think there are thicker ones as well.
Re: Npl fluotar 40L 0.60
Ah okay ,now I understand ,I have to make a sandwich with two slides , one below and one above, measured from 0.6 onwards.apochronaut wrote: ↑Tue Jan 25, 2022 2:51 amUse a measured slide as a coverslip. There are also thick coverslips used for haemocytometers , usually .4 but I think there are thicker ones as well.
Great solution and very simple and practical ,Thanks as always for your great contributions.
Re: Npl fluotar 40L 0.60
Another way is simply use a thick flat well slide (use, for example 1mm of water with or without cover slip), focus with fine focus, then turn cover slip correction collar and select the best position by visual judgement. You can calculate how much water is equivalent to 1mm of glass, for example, but I forgot the formula.
I prefer Apochronaut 's slide as cover slip approach though. You can use a little bit of immersion oil type B or wax around the sides to lift up the "cover slide" very slightly to avoid crushing protists or suffocating everything.
That is a very nice objective, by the way.
I prefer Apochronaut 's slide as cover slip approach though. You can use a little bit of immersion oil type B or wax around the sides to lift up the "cover slide" very slightly to avoid crushing protists or suffocating everything.
That is a very nice objective, by the way.
Re: Npl fluotar 40L 0.60
Excellent solution, very interesting...Of course I will also be putting it into practice.
Thank you!
Thank you!
Re: Npl fluotar 40L 0.60
Thanks for the help.apochronaut wrote: ↑Tue Jan 25, 2022 2:51 amUse a measured slide as a coverslip. There are also thick coverslips used for haemocytometers , usually .4 but I think there are thicker ones as well.
Re: Npl fluotar 40L 0.60
It might be an idea to use the objective for observations in a hanging drop.