Hello forum,
Can somebody educate me on the benefits of this objective (as compared to the 40x/0.85 or 0.95 air versions)? There is very little NA gain from actually oiling the sample.
What is the main point of the iris on the objective? What are the intended uses for this? There are other oil 40x’s with significantly higher NA.
Cheers!
Olympus UPlanAPO 40x/1.00 Oil iris?
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erikwetterskog
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Olympus UPlanAPO 40x/1.00 Oil iris?
Nikon S-Ke - Old goodie
Olympus CX40 - Fluorescence [Selling]
Olympus BX61 - Phase contrast, DIC, fluorescence [Work-in-progress]
Olympus CX40 - Fluorescence [Selling]
Olympus BX61 - Phase contrast, DIC, fluorescence [Work-in-progress]
Re: Olympus UPlanAPO 40x/1.00 Oil iris?
The iris would be useful in darkfield and the objective might be less sensitive to coverslip variations without messing with a correction collar. For brightfield, I'd expect images at 1.00na to be a noticeable step ahead of the dry objective. Also, given the close working distances, this is a 40x you wouldn't worry about accidentally dipping into oil when placed next to something like a 1.4na 100x UPlanApo. Lower power (e.g. 40x) but high N.A. objectives are also useful in getting brighter fluorescence images.
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apochronaut
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Re: Olympus UPlanAPO 40x/1.00 Oil iris?
I don't know the Olympus objective specifcally but I use a Reichert 40X 1.0 oil planapo w./ iris fairly regularly and also have the choice to use a dry 40X planfluor in the same stand. I use them about equally.
It all comes down to a systems approach. A dry objective of sufficient N.A. will do the job just as well and if you aren't going to magnify any more, then why mess with oil but IF you intend to use s higher power oil objective anyway, then an oil immersion 40 or 50 makes life so much easier. Seldom in a session after viewing through a high resolution oil objective do I not want to go back to a wider view, so having a lower magnification oil option is the cat's meow. Oil also offers instant coverslip correction. An equivalent performance dry objective will be very finicky as to that.
The iris can be found equally in dry or immersion objectives and usually just positions the objective towards fluorescence and DF applications.
It all comes down to a systems approach. A dry objective of sufficient N.A. will do the job just as well and if you aren't going to magnify any more, then why mess with oil but IF you intend to use s higher power oil objective anyway, then an oil immersion 40 or 50 makes life so much easier. Seldom in a session after viewing through a high resolution oil objective do I not want to go back to a wider view, so having a lower magnification oil option is the cat's meow. Oil also offers instant coverslip correction. An equivalent performance dry objective will be very finicky as to that.
The iris can be found equally in dry or immersion objectives and usually just positions the objective towards fluorescence and DF applications.
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erikwetterskog
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Re: Olympus UPlanAPO 40x/1.00 Oil iris?
Hello,
Thanks for your smart answers! I’m not sure what the price would be, but maybe it’s a bargain.
Ideally I’m looking for a UIS2 60x and 100x since I have the DiC-prisms for those. But they do cost some serious $$$.
Thanks for your smart answers! I’m not sure what the price would be, but maybe it’s a bargain.
Ideally I’m looking for a UIS2 60x and 100x since I have the DiC-prisms for those. But they do cost some serious $$$.
Nikon S-Ke - Old goodie
Olympus CX40 - Fluorescence [Selling]
Olympus BX61 - Phase contrast, DIC, fluorescence [Work-in-progress]
Olympus CX40 - Fluorescence [Selling]
Olympus BX61 - Phase contrast, DIC, fluorescence [Work-in-progress]
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Macro_Cosmos
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Re: Olympus UPlanAPO 40x/1.00 Oil iris?
1. Higher RI so that NA 1 oil will be brighter than an NA 0.95 dry, most immersion oil RI ~ 1.52 (e-line), air ~1.00. This means a brighter fluorescence signal and better matched RI to the glass slip.
2. The 1.0 oil one does not care about the thickness of your coverslip (well unless it is thicker than the working distance), the 0.95 etc air ones do.
3. Iris, you can use a darkfield patch stop.
4. This objective is a uis era old one, it has been superceded. During the time it was in production, there were no higher NA immersion 40x apochronatic objectives, the UApos do not count.
2. The 1.0 oil one does not care about the thickness of your coverslip (well unless it is thicker than the working distance), the 0.95 etc air ones do.
3. Iris, you can use a darkfield patch stop.
4. This objective is a uis era old one, it has been superceded. During the time it was in production, there were no higher NA immersion 40x apochronatic objectives, the UApos do not count.
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erikwetterskog
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Re: Olympus UPlanAPO 40x/1.00 Oil iris?
I ended up buying it, in a package with a PlAPO 100x and a 40x 0.95 dry.
Can’t wait to try them out! Good point regarding having two different oil objectives with different magnification. Now my only dilemma what I have to leave outside the nosepiece.
Can’t wait to try them out! Good point regarding having two different oil objectives with different magnification. Now my only dilemma what I have to leave outside the nosepiece.
Nikon S-Ke - Old goodie
Olympus CX40 - Fluorescence [Selling]
Olympus BX61 - Phase contrast, DIC, fluorescence [Work-in-progress]
Olympus CX40 - Fluorescence [Selling]
Olympus BX61 - Phase contrast, DIC, fluorescence [Work-in-progress]
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apochronaut
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Re: Olympus UPlanAPO 40x/1.00 Oil iris?
Some companies supplied 7 place nosepieces, which solve some problems of how to most efficiently gang objectives. Another perhaps better method is to have changeable nosepieces, with one for oil immersion including DF N.A. reduction options and another with a dry array, incuding a high N.A. 60-63X and W.F. 15X eyepieces. It can get expensive but with systems that aren't on everyone's radar, not too bad.
A best go to system in a 6 place nosepiece would hopefully carry pretty high N.A. objectives from 20X to 50X, so you could use W.F. 15X eyepieces to extend the system.
4X .10 to .13, 10X .25 or better, 20-25X .50 to .75, 40X 65 to .95 dry, 40-50X .95 to 1.4 oil preferably w./iris and 100X 1.25 to 1.45 oil preferably w./iris.
that would give you 40X, 60X, 100X, 150X, 200X or 250X, 300X or 375X, 400X or 500X, 600X or 750X and 1000X. If your 100X N.A. is high enough ( 1.4 or 1.45) and especially if apochromatic you can probably use the 15X eyepieces at 1500X with little loss of resolution.
I occasionally use a rare Bausch & Lomb 125X 1.4 N.A. Flat Field apochromat with 15X W.F. eyepieces at an astounding 1725X and the resolution dropoff although there, is not huge. With 12.5X eyepieces at 1562.5X , it is negligable. Apochromats can exceed the 1000X the N.A. maximum magnification rule by about 10%, so 1100X1.4 = 1540X, with a 1.4 N.A. objective.
A best go to system in a 6 place nosepiece would hopefully carry pretty high N.A. objectives from 20X to 50X, so you could use W.F. 15X eyepieces to extend the system.
4X .10 to .13, 10X .25 or better, 20-25X .50 to .75, 40X 65 to .95 dry, 40-50X .95 to 1.4 oil preferably w./iris and 100X 1.25 to 1.45 oil preferably w./iris.
that would give you 40X, 60X, 100X, 150X, 200X or 250X, 300X or 375X, 400X or 500X, 600X or 750X and 1000X. If your 100X N.A. is high enough ( 1.4 or 1.45) and especially if apochromatic you can probably use the 15X eyepieces at 1500X with little loss of resolution.
I occasionally use a rare Bausch & Lomb 125X 1.4 N.A. Flat Field apochromat with 15X W.F. eyepieces at an astounding 1725X and the resolution dropoff although there, is not huge. With 12.5X eyepieces at 1562.5X , it is negligable. Apochromats can exceed the 1000X the N.A. maximum magnification rule by about 10%, so 1100X1.4 = 1540X, with a 1.4 N.A. objective.