Objective repair,need help

Everything relating to microscopy hardware: Objectives, eyepieces, lamps and more.
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92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Objective repair,need help

#1 Post by 92111 » Fri Sep 28, 2018 7:25 am

i can't speak English
hello,every one,i have a good leitz npl normal Objective,and a bad phase contrast Objective, i want to get a good phase contrast Objective finally ,can i?,if yes,give me some suggestions,thanks everyone!
here are my Objectives.
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apochronaut
Posts: 6411
Joined: Fri May 15, 2015 12:15 am

Re: Objective repair,need help

#2 Post by apochronaut » Fri Sep 28, 2018 1:11 pm

In theory, yes. There are several things to consider , though.

Many phase objectives are just as you are surmising, the same as the BF version , just with a phase ring( annulus) installed at a critical point. Usually, it is not that hard to remove the optical element containing the annulus from one objective and putting it into the other.

However, a couple of details need to be carefully observed and for this reason, it is best to remove as much of the lens pack from the donor objective as is possible, leaving behind only the defective lens(es). In your case, I would replace the entire lens pack, up to the front lens, as a unit.

Objectives usually have shims in them. These are used to correct for small differences in the refractive index of various glass batches. The shimming thickness between the various lens elements can vary in two otherwise identical objectives, so you want to move as much of one lens pack in it's entirety as possible to the other barrel.

Remove both lens packs in their entirety being careful to mark both the barrel and the lens pack, so the lens pack goes back into the barrel in the exact rotational location. Keep the rotational alignment of the lens pack intact. There may be alignment marks scribed down the sides of each lens pack. Swap the good front lens cell, complete with the shim behind it( if there is one) over to the phase lens pack. You can't do anything to ensure the rotational alignment of the new front lens but it is shallow, so it is therefore likely to be fairly uniform. Install the renewed phase lens pack in the correct marked location into the phase barrel. Make sure to use the original shim from behind the donor front lens.

good luck with that and send us some pictures of your phase images, if the transplant is successful.

Hobbyst46
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Re: Objective repair,need help

#3 Post by Hobbyst46 » Fri Sep 28, 2018 2:30 pm

From the photos, there are obvious mechanical flaws in the metal parts of the Phaco, and it appears that by someone has tried to remove the back lens-retaining ring with some (inappropriate) tool, but stopped short of the job.
Besides these flaws, what is wrong with the "bad" objective ? are the optics damaged for sure ? can the inner barrel move under slight pressure (is it spring loaded?)

MicroBob
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Location: Northern Germany

Re: Objective repair,need help

#4 Post by MicroBob » Fri Sep 28, 2018 6:14 pm

These objectives look similar, but viewed from the back there is a difference in the plate holding the back lens. This makes it less likely that you will end up with a working objective. If you have the opportunity to sell your normal objective and buy a phase version, you might choose the safer way. I would be worried to end up with two objectives that don't perform well. This a already a highly corrected objective with 10 or so lenses and a couple of distancers.

apochronaut
Posts: 6411
Joined: Fri May 15, 2015 12:15 am

Re: Objective repair,need help

#5 Post by apochronaut » Sun Sep 30, 2018 8:39 pm

As long as the lens packs are swapped in their entirety, minus the offending front lens, that difference won't be a problem, presuming that the barrel i.d. And lens cell o.d. Have not been changed.

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#6 Post by 92111 » Mon Oct 01, 2018 7:43 am

sorry everyone,i reply late,i baught a tool on line(on the way),i am plan to Disassembly the bad one,then decide weather Disassembly the good one.it sence very difficult for me
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92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#7 Post by 92111 » Mon Oct 01, 2018 11:58 am

hello everyone
my job paused here now, the phase ring is too deep to get out.maybe its time to try MicroBob's advice
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Last edited by 92111 on Mon Oct 01, 2018 1:45 pm, edited 1 time in total.

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#8 Post by 92111 » Mon Oct 01, 2018 12:44 pm

i tried my best ,but can't dismantle it totally ,i failed to open the yellow one.
thanks everyone,
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apochronaut
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Re: Objective repair,need help

#9 Post by apochronaut » Mon Oct 01, 2018 2:53 pm

not sure what you did there. Did you drill two holes in the front of the objective? not a good idea. The set ring in the back , keeps everything together. Older objectives can get stiff and it takes a little gentle persuasion to push the lens pack through from the front to the back.

If you want to send them to me , I'll get the insides swapped over. Just pay the postage., both ways.

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#10 Post by 92111 » Mon Oct 01, 2018 3:48 pm

apochronaut wrote:not sure what you did there. Did you drill two holes in the front of the objective? not a good idea. The set ring in the back , keeps everything together. Older objectives can get stiff and it takes a little gentle persuasion to push the lens pack through from the front to the back.

If you want to send them to me , I'll get the insides swapped over. Just pay the postage., both ways.
thanks for your prompt,i get a set ring now,there still another set ring.
i am in china,postage is very expensive,the bad objective worth it?

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#11 Post by 92111 » Mon Oct 01, 2018 3:58 pm

apochronaut wrote: takes a little gentle persuasion to push the lens pack through from the front to the back.
i got the phase ring now ,but i forget the rotational alignment,the rotational alignment just + and - ?or 0 to 360 degree
sorry apochronaut,i am afraid that i can't send you now
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92111
Posts: 170
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Re: Objective repair,need help

#12 Post by 92111 » Mon Oct 01, 2018 4:23 pm

apochronaut wrote:In theory, yes.

good luck with that and send us some pictures of your phase images, if the transplant is successful.
i did it,i will test it right now
thanks
thanks very much
it works well?
yeast phase contrast 100x10x
why the background in the smartphone is different with the background in eyepiece?
dear apochronaut are you here?
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92111
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Re: Objective repair,need help

#13 Post by 92111 » Mon Oct 01, 2018 5:02 pm

apochronaut wrote: if the transplant is successful.
is the transplant successful?if yes,ok the lens of the bf objective send to you to thanks you,if your think it worth the postage !
here they are
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apochronaut
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Re: Objective repair,need help

#14 Post by apochronaut » Tue Oct 02, 2018 1:35 pm

92111 wrote:
apochronaut wrote:In theory, yes.

good luck with that and send us some pictures of your phase images, if the transplant is successful.
i did it,i will test it right now
thanks
thanks very much
it works well?
yeast phase contrast 100x10x
why the background in the smartphone is different with the background in eyepiece?
dear apochronaut are you here?
It seems that you have made the changeover for the annulus( phase ring) at least. It is possible that is all you need. to do

The background in your picture, does show a different colour than it should but it sounds like that is not the colour you are seeing through the eyepieces. Are you getting a more grey colour?

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#15 Post by 92111 » Wed Oct 03, 2018 12:44 am

apochronaut wrote: The background in your picture, does show a different colour than it should but it sounds like that is not the colour you are seeing through the eyepieces. Are you getting a more grey colour?
yes.in eyepiece the colour is gray.but it is green in smart phone.my phone always made wrong colour.for take photo which is batter between smartphone to camera?does my microscope the leitz diavert support 30mm leitz trinocular-head or just support 23mm trinocular-head .does this camera system suit for me?viewtopic.php?f=9&t=5557.
it seams that my microscope can adjust the size of eyesight,right?
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92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#16 Post by 92111 » Wed Oct 03, 2018 2:14 am

what the difference between the two leitz trinocular head?i love the black one.but it can work on my microscope?i saw every other leitz diavert is white one on google img.
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92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#17 Post by 92111 » Wed Oct 03, 2018 2:19 am

Hobbyst46 wrote:From the photos, there are obvious mechanical flaws in the metal parts of the Phaco, and it appears that by someone has tried to remove the back lens-retaining ring with some (inappropriate) tool, but stopped short of the job.
Besides these flaws, what is wrong with the "bad" objective ? are the optics damaged for sure ? can the inner barrel move under slight pressure (is it spring loaded?)
hello Hobbyst46.my job completed.the flaws is made by me by unright tool.and the front len is break os the phase one
Last edited by 92111 on Wed Oct 03, 2018 2:27 am, edited 1 time in total.

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#18 Post by 92111 » Wed Oct 03, 2018 2:24 am

MicroBob wrote:These objectives look similar, but viewed from the back there is a difference in the plate holding the back lens. This makes it less likely that you will end up with a working objective. If you have the opportunity to sell your normal objective and buy a phase version, you might choose the safer way. I would be worried to end up with two objectives that don't perform well. This a already a highly corrected objective with 10 or so lenses and a couple of distancers.
hello MicroBob and thanks for your advice.it is hard for me to buy phase version in china .the changover is easy to do.but it is hard for the first time.the lenes seens only five .thanks again

apochronaut
Posts: 6411
Joined: Fri May 15, 2015 12:15 am

Re: Objective repair,need help

#19 Post by apochronaut » Wed Oct 03, 2018 10:57 am


92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#20 Post by 92111 » Thu Oct 04, 2018 12:45 am

apochronaut wrote:here is a manual for the Diavert. http://www.micromagus.net/Microdocs/diavert_manual.pdf
i have that manual.but it said nothing about that adjuster.forget it.i decide to by the white one.thanks for your help,apochronaut.,and best wish to you

MicroBob
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Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: Objective repair,need help

#21 Post by MicroBob » Thu Oct 04, 2018 1:59 am

Nice to hear that your work was sucessful!
These Leitz lenses are really good and not prone to delamination.

Grahame
Posts: 99
Joined: Fri Jun 29, 2018 7:42 am
Location: New Zealand

Re: Objective repair,need help

#22 Post by Grahame » Thu Oct 04, 2018 6:20 am

Hi 92111,
The strange colour you are getting from your smartphone will be a colour balance problem.
I don't know what the light source for the Diavert is, I guess halogen.
Your phone is probably set to daylight.
Just change it in the phone's menu.

92111
Posts: 170
Joined: Thu Jul 26, 2018 8:45 am

Re: Objective repair,need help

#23 Post by 92111 » Thu Oct 04, 2018 8:32 am

Grahame wrote:Hi 92111,
Your phone is probably set to daylight.
Just change it in the phone's menu.
Grahame,my phone don't have daylight,i download an camera app and change the settings,but still failed,may be my phone is too old.thank you and don't care about this problem ,i plan to buy a Trinocular Head

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