tissue sample

[MicroInspector]

10 x 10x

This is a tissue sample from chicken
I don't know if you fellow microscopist have tried this

[mrsonchus]

I hate to say this, but that just looks like a blur to me old chap - you'll need to give us an image that actually looks like something for any constructive comments. Sorry to be critical my friend.

[billbillt]

I agree with John.. You need to do more to try to improve on this.. This photo looks like nothing... You might try doing a thin section and stain it and go to higher mag..

BillT

[MicroInspector]

I keep trying

[billbillt]

Take a look at some of the work that others are posting... Practice until you can closely duplicate it..

BillT

[MicroInspector]

But I just got started in this
This Pay Day I be getting a smart phone adopter for my microscope, as for stains I have to try iodine or food coloring until I can get the stains.
Still have to get isopropyl anchol and lens cleaning cloth an eye dropper and tweezers a razor craft knife.

[JimT]

MicroInspector , looks like a blur to me also but don't be discouraged or quit. Some hard feed back but good job even on getting an image and "Sticking your neck out" to post it. You should see some of my first images!

Looking forward to more images with your adopter.

JimT

[mrsonchus]

MicroInspector , looks like a blur to me also but don't be discouraged or quit. Some hard feed back but good job even on getting an image and "Sticking your neck out" to post it. You should see some of my first images!

Looking forward to more images with your adopter.

JimT

That Jim is good advice! The posting of first images is always a terrifying time! I too remember some of the horrors I produced as I started - the fine folks here helped me along, as they will MicroInspector for certain.

[gekko]

I would suggest that you try something like a hair or a very small piece of tissue paper.

[MicroInspector]

I try maybe some insect blood

[MicroInspector]

Well after understanding what you guys talking about
Here red onion skin sample
And try to take better photos
Tell what you think?
[/URL1
10x 10x


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10x 4


25x4[quote][/quote]


25x10x


25x40

[billbillt]

This is somewhat better than your last photos... Still needs practice...

BillT

[mrsonchus]

As Bill said - your choice of the onion skin was a good one to help you to get it right.
Your next step is to make sure the camera is steady, level and focused. If you stick to simple, see-through, flat and above all thin (so that light can go through it to your lens) samples you should begin to get better.
Forget anything from an animal for now - animal tissue is far harder to image than plant.

Practice - you'll get there if you do.

[mrsonchus]

oops - posted twice!

[rnabholz]

I take it from the note on the images that you are using 25x eyepieces?

If you have 10x eyepieces I would strongly recommend using them instead. The exit pupil of the higher magnification make lining up the camera much more difficult.

Try 10x eyepieces with your 10x objective. Start with low power, master that, and then increase. The goal is photos that look as sharp as you see with your own eyes at the eyepiece.

[apochronaut]

But I just got started in this
This Pay Day I be getting a smart phone adopter for my microscope, as for stains I have to try iodine or food coloring until I can get the stains.
Still have to get isopropyl anchol and lens cleaning cloth an eye dropper and tweezers a razor craft knife.

The pictures are a good start.
That is what all of your critics had as images, probably not even as good at one time. Tissue is hard to image because there is little contrast.
Try turmeric as a stain, it usually works well. Sometimes paprika will work, depending on from which peppers it was made and by who. Look for Spanish or Hungarian. The Israeli stuff is garbage. Saffron is generally expensive but there is a lousy cheap one from China, that is widely available. It is terrible saffron but makes a decent dye.
Other possibilities are to cook a small beet, or a small amount of red cabbage in little water. You will get a strong red dye from the beet and a blue dye from the cabbage but unless they are fixed somehow, they will fade. Maybe extracting a little from the vegetable tissue with alcohol would work, I haven't tried it. They might make a good temporary stain, nonetheless.

[MicroInspector]

Thank you
I have a smartphone adopter to take better photos
Would it be good tenure of iodine?

[MicroInspector]

This is old blood stain from cloth interria the was a 1970 ford escort this was in a head on collision.
The driver head his on windshield
And some blood flew on to cloth interria in the car.
I got spiecemen back when I lived a collusion savage yard and when I had my Tasco 100 microscope
Now this spiecemen under my new microscope
This is small piece of the car's cloth interria.
The driver's blood stain


10x 40x



10x 40x



10x 40x

[MicroInspector]

Well what you think about this report???

[MicroInspector]

No your not looking at the planet Mars
This is the skin of a old plum
Look before your eyes.


10x4x


10x 10x


10x 40x


20x 4x



20x10x

[billbillt]

Yes.. These are much better!!... " Practice makes perfect"...

[MicroInspector]

Plus the use of a home made thing of bobber

[c-krebs]

And try to take better photos
Tell what you think?

I'm new here and not familiar with your "hardware". Are there any posts that provide some info on the equipment you are using?

[MicroInspector]

I use a home made thing to take better photos
Until I get my carson universal smartphone adopter.

[billben74]

These are getting better.
Keep trying and keep posting.
Hair is a good thing to have a go at -> it was the first thing I tried.

One fairly cheap adapatation for hair is get linear polarising filters.

e.g. https://www.amazon.com/Polarizing-Film- ... ng+plastic

You then need one circle of the sheet in the head of the microscope, where the light comes from the lens tube. You need another filter to cover the light coming out of the light source.
Turn the sheet/circle cut from the sheet until the background goes black.
Some of the hairs will display bright colours.

Anyway, keep going you are getting more skillful already.

[molehill]

MicroInspector, what you are probably not appreciating (when I started out I didn't either) is how thin your specimens need to be.
The cover slips we use are about 0.17mm thick. Specimens need to be at least ten times thinner then the cover slip to produce good images
Your objectives will be corrected for a coverslip thickness of 0.17 this is in fact the thickness of the entire preparation cover slip including specimen.
Low magnification objectives are more tolerant of thicker preparations but by the time you reach 40x they need to be spot on. You don't notice this at
first because you have nothing to compare it with, but as you gain experience you will start to appreciate what a good image looks like.

molehill