The Art of Fluorescence Microscopy (DAPI filter)

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bkwoo
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The Art of Fluorescence Microscopy (DAPI filter)

#1 Post by bkwoo » Fri Aug 14, 2020 10:30 am

Hello All
I am afraid I can only do pictures (fulfilled quota of 5 pictures) and not video recording (referring to Introduce yourself- "Hello... from Kuala Lumpur, Malaysia").
Zeiss Axionet Ax10 fluorescence microscope being used here.

The first 3 are mosquito larva, and mosquito larva's food ( a microscopic worm?? :o :roll: ) or it could just be a tiny piece of cloth cotton fiber. Who knows :?: :!:
4th is just onion epidermal cells.

The last one is tadpole's body and tail. At least for this picture, there is no "microscopic worm" or "cloth cotton fiber" :oops: lurking around. :)

Enjoy! ;)
Attachments
mosquito larva head.jpg
mosquito larva head.jpg (31.47 KiB) Viewed 10628 times
mosquito larva tail.jpg
mosquito larva tail.jpg (29.76 KiB) Viewed 10628 times
mosquito larva BW.jpg
mosquito larva BW.jpg (34.42 KiB) Viewed 10628 times
onion.jpg
onion.jpg (32.14 KiB) Viewed 10628 times
tadpole tail together.jpg
tadpole tail together.jpg (24.39 KiB) Viewed 10628 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#2 Post by bkwoo » Thu Aug 20, 2020 3:17 am

continuing....

1st: Mosquito pupa's head.
2nd: "Brown mite", generic name because I do not know what that is :?: :roll:, another milimetre-scale pond creature. Smallest milimetre-scale pond creature here. Maybe we squished it hard :?: and the organelles spilled out ? :?: :o
3rd: "Black Aphid", generic name again :roll: :?: , this milimetre-scale pond creature is like a water cockcroach? :?: , a hexapod?? :?: , it floats and dashes fast on the water surface. Not a submariner. :)
4th: "Black Aphid" merged brightfield and fluorescence picture.
5th: From top to bottom: onion epidermal cells layer (learning this from Oliver's Youtube video :lol: ), mosquito larva, mosquito pupa, tadpole and "Brown mite". :)

Enjoy viewing! ;)
Attachments
pupa head.jpg
pupa head.jpg (33.77 KiB) Viewed 10579 times
brown mite.jpg
brown mite.jpg (42.81 KiB) Viewed 10579 times
black aphid body.jpg
black aphid body.jpg (20.61 KiB) Viewed 10579 times
aphid head merge 10x_c1+2.jpg
aphid head merge 10x_c1+2.jpg (45.23 KiB) Viewed 10579 times
4slides.jpg
4slides.jpg (36.04 KiB) Viewed 10579 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#3 Post by bkwoo » Mon Aug 31, 2020 12:25 am

Today is a special day here in Malaysia. A fine day for posting :) .

First 3 pictures: From E. Coli culture (Leica DM2500 fluorescence microscope).

4th: Tissue section buccal mucosa (Nikon H600L Eclipse 90i fluorescence microscope).

Last picture: Yakult Ace probiotic drink microbes (Nikon Eclipse 90i fluorescence microscope).

Enjoy viewing as usual!! :)
Attachments
"This is a big f#@king signal" "12 meters, 11, 10, 9 meters, 8 meters, 7, 6, I am reading right, man, look" "5 meters, man, 4, What the hell? "Oh my god!"
"This is a big f#@king signal" "12 meters, 11, 10, 9 meters, 8 meters, 7, 6, I am reading right, man, look" "5 meters, man, 4, What the hell? "Oh my god!"
P_20190208_1114114040.jpg (78.3 KiB) Viewed 10525 times
&quot;You tell me man, I only work here&quot;<br />&quot;What is that?&quot;<br />&quot;I don't know&quot;<br />&quot;I am not sure&quot;<br />&quot;It must be something we haven't seen yet&quot;<br />&quot;Maybe it is like an ant's hive&quot; &quot;You know what I mean&quot; &quot;It is like one female who runs the whole show&quot; &quot;Yeah, the momma&quot;
"You tell me man, I only work here"
"What is that?"
"I don't know"
"I am not sure"
"It must be something we haven't seen yet"
"Maybe it is like an ant's hive" "You know what I mean" "It is like one female who runs the whole show" "Yeah, the momma"
baccoloniesred33.jpg (58.56 KiB) Viewed 10525 times
&quot;They are coming off from the god-d@mmned wall!!&quot;
"They are coming off from the god-d@mmned wall!!"
redabcombined33.jpg (106.59 KiB) Viewed 10525 times
&quot;Yo! Stop your grinning and drop your linen&quot; &quot;Found them&quot; <br />&quot;It looks like all of them&quot; &quot;Over the processing station&quot; &quot;Sub level 3 under the main cooling tower&quot;
"Yo! Stop your grinning and drop your linen" "Found them"
"It looks like all of them" "Over the processing station" "Sub level 3 under the main cooling tower"
dr woo_01-04_280619105857.jpg (43.32 KiB) Viewed 10525 times
&quot;Check those corners&quot; &quot;Steady people, let finish our sweep&quot;<br />&quot;We are still MicrobeHunters??!!, and we got a job to do&quot;<br />&quot;Movement&quot;&quot;What is the position&quot; &quot;Ergh..Can't lock in&quot;<br />&quot;Multiple signals&quot; &quot;They are closing!&quot;<br />&quot;Go UV? people&quot; &quot;Look Sharp&quot; &quot;I got signals I got readings, in front and behind&quot;<br />&quot;Tracker is off the scale, man&quot;<br />&quot;They are all around us, man!&quot;<br />&quot;Maybe they don't show up on UV? at all&quot;<br />&quot;Maybe we got them demoralized&quot;
"Check those corners" "Steady people, let finish our sweep"
"We are still MicrobeHunters??!!, and we got a job to do"
"Movement""What is the position" "Ergh..Can't lock in"
"Multiple signals" "They are closing!"
"Go UV? people" "Look Sharp" "I got signals I got readings, in front and behind"
"Tracker is off the scale, man"
"They are all around us, man!"
"Maybe they don't show up on UV? at all"
"Maybe we got them demoralized"
sumyakult3mh.jpg (49.68 KiB) Viewed 10525 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#4 Post by bkwoo » Fri Sep 18, 2020 1:56 pm

Damselfly larvae, Zeiss AX10 inverted fluorescence microscope.
BF is brightfield, FL is fluorescence, PH is phase contrast. 5x is magnification.
Enjoy viewing!
Attachments
BFandFL5x.jpg
BFandFL5x.jpg (30.72 KiB) Viewed 10274 times
BFandFL20x.jpg
BFandFL20x.jpg (31.56 KiB) Viewed 10274 times
fl+bf_merge 20x_c1+2.jpg
fl+bf_merge 20x_c1+2.jpg (72.29 KiB) Viewed 10274 times
ph 20x.jpg
ph 20x.jpg (89.25 KiB) Viewed 10274 times
ph 5x.jpg
ph 5x.jpg (95.35 KiB) Viewed 10274 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#5 Post by bkwoo » Sun Sep 20, 2020 3:51 am

Penetrating the pond sub-milimetre scale creatures' domain, sooner or later, after halting at pond milimetre-scale creatures.

Remmber, that I am not a biology major, so microorganisms species identification is not my forte, so I will call this, Copepod.

Copepod, Zeiss AX10 inverted fluorescence microscope.

The first 2 pictures, are the initial frontal view that we caught.

The last 3 pictures, in our excitement, we touched the coverslip, so the sample is moved to a more lateral view. I am assuming those round globular clusters are "multinucleus".

Enjoy viewing!
Attachments
BFandFL.jpg
BFandFL.jpg (25.87 KiB) Viewed 10222 times
A fluoro 4.jpg
A fluoro 4.jpg (21.24 KiB) Viewed 10222 times
A BF 10 x.jpg
A BF 10 x.jpg (70.05 KiB) Viewed 10222 times
A fluoro.jpg
A fluoro.jpg (29.75 KiB) Viewed 10222 times
A fluoro and bf-merge_c1+2.jpg
A fluoro and bf-merge_c1+2.jpg (54.35 KiB) Viewed 10222 times
Last edited by bkwoo on Sat Oct 03, 2020 1:07 pm, edited 1 time in total.
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#6 Post by bkwoo » Mon Sep 21, 2020 4:30 am

Entering the realm of worms!
Yes, worms as in giant monster worm in hollywood movie, Tremors (1990) or that giant sandworm on planet Arrakis. :)



Forewarned. I hope the pictures are not too scary for kids. :roll:

Stylaria (this time I hope I got this right, :) ), Zeiss A10 inverted fluorescence microscopy.
Enjoy viewing! :)
Attachments
cacing BFandFL10x.jpg
cacing BFandFL10x.jpg (29.68 KiB) Viewed 10180 times
cacing BFandFL20x.jpg
cacing BFandFL20x.jpg (23.55 KiB) Viewed 10180 times
PHandF 5x.jpg
PHandF 5x.jpg (41.27 KiB) Viewed 10180 times
fl 20x.jpg
fl 20x.jpg (23.83 KiB) Viewed 10180 times
fl+bf_merge 20x_c1+2.jpg
fl+bf_merge 20x_c1+2.jpg (62.21 KiB) Viewed 10180 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#7 Post by bkwoo » Tue Sep 22, 2020 5:39 am

Last post. Last 5 pictures with insets.

1st: Originally 5 slides with 5 samples were intended. We lost one due to carelessness. Amateur do the amateur things :shock: . So 4 made it through the photomicrography session. They are: Copepod, Worms, one small damselfly larvae (refer previous post) and now this, one big damselfly larvae. Inset is the 5 slides with 5 samples amidst the collection of pond milimetre-scale creatures, most are damselfly larvaes and the worms. Can you see the worms? :lol:

2nd: Big damselfly larvae's head. BF and FL. We focussed on the small blue dots-bacterias? :o on its head. Symbiotic relationship between bacteria-microbes and damselfly larvae-pond milimetre-scale creature? :?: :roll:

3rd: Big damselfly larvae's tail. BF and FL. There is a fluorescent pattern on the middle tail that brightfield cannot catch it. :)

4th: Big damselfly larvae's brightfield and fluorescence combined. Inset is the two slides after photomicrography session: Slide C is the small damselfly larvae and slide B is the big damselfly larvae for size comparison. Coverslip is 18 mm x 18 mm (Deckglaser). We never bother with coverslip on slide B. :lol:

5th: Copepod in phase contrast. Notice the blur part on the bottom part of the copepod. Guess biological microscopic lifeform such as this copepod, knows how to keep its secret. ;) :P

Thank you all for viewing... :) until next time.
Attachments
mmsample.jpg
mmsample.jpg (91.28 KiB) Viewed 10150 times
HeadBFandFL5x.jpg
HeadBFandFL5x.jpg (29.93 KiB) Viewed 10150 times
TailBFandFL5x.jpg
TailBFandFL5x.jpg (30.65 KiB) Viewed 10150 times
2slidestail fl +bf  merge 5x-Image Export-05_c1+2.jpg
2slidestail fl +bf merge 5x-Image Export-05_c1+2.jpg (68.12 KiB) Viewed 10150 times
PH.jpg
PH.jpg (37.9 KiB) Viewed 10150 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#8 Post by Wes » Tue Sep 22, 2020 6:34 am

Cool but can you actually buy some DAPI? You could then see the DNA inside microorganisms for example the different nuclei in ciliates.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#9 Post by bkwoo » Sat Oct 03, 2020 1:41 pm

A journey into biological microscopic heartland in a world that is ravaged by Covid-19.

We are exploring pond sub-milimetre scale creatures (include pond protists, pond crustaceans, pond protozoas, pond zooplanktons, etc..excluding diatoms and microalgae) found in Kuala Lumpur, Malaysia.

First off, Malaysia has a hot, hot equator weather. Kuala Lumur is one location, one tend to find rainfall around 3 to 6 pm, almost everyday in a week. So it is hot and humid all year round like having summer the whole year. ;)

So far, post Covid-19 microscopy activities have been onion epidermal cell layers, pond milimetre-scale creatures (mosquito larva, pupa, tadpole, "Black aphid", damselfly larvaes and worms) and pond microcrustacean, copepod. Oh..yeah..copepod is not a protist. :)

Looking back, I have mishandled the "Brown mite" sample. One should not put two biological samples on same slide with huge differences. One thick and big:tadpole and another thin and small: "Brown mite". :oops:

1st: Pond microcrustacean A, BF and FL, 10x. Design feature for the next alien monster movie from Hollywood. A two-limbed slasher alien stalker? :lol:

2nd: FL merged BF, 10x
3rd: FL merged BF, 20x
4th: FL merged PH, 10x
5th: Pond microcrustacean A, BF and FL, 20x

Enjoy viewing!! :)

to be continued....
Attachments
Protist a.jpg
Protist a.jpg (26.82 KiB) Viewed 9798 times
Protist a fluoro  + bf  10x_c1+2.jpg
Protist a fluoro + bf 10x_c1+2.jpg (61.51 KiB) Viewed 9798 times
Protist a fluoro  + bf20x_c1+2.jpg
Protist a fluoro + bf20x_c1+2.jpg (88.32 KiB) Viewed 9798 times
Protist a fluoro  + ph 10x_c1+2.jpg
Protist a fluoro + ph 10x_c1+2.jpg (130.2 KiB) Viewed 9798 times
Protist a  bf and Fl 20x.jpg
Protist a bf and Fl 20x.jpg (36.84 KiB) Viewed 9798 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#10 Post by bkwoo » Sun Oct 04, 2020 6:38 am

1st: Pond microcrustacean a, FL merged PH, 20x

2nd: Cylopoida copepoda (adult) and smaller copepodite, 5x
3rd: BF merged FL, 5x
4th: FL
5th: FL merged PH, 5x

Enjoy viewing!

to be continued...
Attachments
Protist a fluoro  + ph 20x_c1+2.jpg
Protist a fluoro + ph 20x_c1+2.jpg (93.55 KiB) Viewed 9764 times
aa bfandFL 5x.jpg
aa bfandFL 5x.jpg (24.54 KiB) Viewed 9764 times
aa bf + fluro 5x_c1+2.jpg
aa bf + fluro 5x_c1+2.jpg (56.31 KiB) Viewed 9764 times
aa fluro.jpg
aa fluro.jpg (22.84 KiB) Viewed 9764 times
aa ph + fluro 5x_c1+2.jpg
aa ph + fluro 5x_c1+2.jpg (86.62 KiB) Viewed 9764 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#11 Post by bkwoo » Mon Oct 05, 2020 6:45 am

1st: Small copepodite's body (previous posting)

2nd: Cyclopoida copepod, BF and FL
3rd: Same as above, focusing on its eye, 5x
4th: BF merged FL
5th: BF merged FL, eye, 5x

Enjoy viewing!

to be continued...
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aa 1 fluro.jpg
aa 1 fluro.jpg (26.26 KiB) Viewed 9731 times
bw bfandFL.jpg
bw bfandFL.jpg (24.64 KiB) Viewed 9731 times
bw mata bfandFL 5x.jpg
bw mata bfandFL 5x.jpg (26.85 KiB) Viewed 9731 times
bw bf+ fluro_c1+2.jpg
bw bf+ fluro_c1+2.jpg (58.48 KiB) Viewed 9731 times
bw mata bf + fluro 5x_c1+2.jpg
bw mata bf + fluro 5x_c1+2.jpg (64.01 KiB) Viewed 9731 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#12 Post by bkwoo » Tue Oct 06, 2020 2:36 am

1st: Cyclopoida copepod (previous posting), FL merged PH, eye part, 5x

2nd: Mixed cladocera. Daphnia/Moinids/Sidids. BF and FL
3rd: As above, BF merged FL
4th: PH merged FL

5th: Cladoceran ephippia, Sididae family?, BF and FL, the eggs, they all lit up like a christmas tree! :)

Enjoy viewing! ;)

....to be continued.....
Attachments
bw mata ph + fluro 5x_c1+2.jpg
bw mata ph + fluro 5x_c1+2.jpg (92.36 KiB) Viewed 9706 times
c bfandFL.jpg
c bfandFL.jpg (29.79 KiB) Viewed 9706 times
c bf + fluro_c1+2.jpg
c bf + fluro_c1+2.jpg (75.2 KiB) Viewed 9706 times
c ph + fluro_c1+2.jpg
c ph + fluro_c1+2.jpg (113.28 KiB) Viewed 9706 times
cb FLandbf.jpg
cb FLandbf.jpg (29.9 KiB) Viewed 9706 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#13 Post by bkwoo » Wed Oct 07, 2020 3:37 am

I collected my first Kuala Lumpur pond water samples, my first outdoor biological fieldwork experiences was on this year's June, once the lockdown was lifted. Of course, after going through Oliver Kim's Youtube videos on collecting pond water samples and making some preparations. :)

So a little bit of information about Kuala Lumpur's ponds. They are seasonal with short cycles. They do dried up, then after several days of rainfall, water is filled back in and pond's life springs back forth. Guess this cycle has been going on since planet earth has this peninsular land protruding out from Asia mainland. So far I have seen only the pond water turns cloudy. I am guessing that is just a bacteria bloom? rather than an algal bloom. There are no outdoor ice-skating activities here in Malaysia or in Thailand, Vietnam, the Philippines, Indonesia or other South East Asia nations. :)

There is a mangrove forest along the coastline near Kuala Lumpur, famous for its fireflies, the Kuala Selangor area where one should be able to find microalgae and diatoms and a lots more of richly-diversified biological (aquatic, insect lifeforms, etc..and plant) samples but right now, I am not planning to go there. :)

Okay let get back to the pictures.

1st: Cladoceran ephippia (from previous posting), concentrating on the eggs. BF and FL.

2nd: Zoomed up of the eggs, FL merged PH. Another design feature for the brain's part for Brainiac, the supervillain in his robotic mechanical suit. Another upcoming DC comics movie from Hollywood? :lol:

3rd: The eggs, FL merged BF.

4th: Cladoceran ephippia, FL merged BF.

5th: As above, FL merged PH.

We are almost coming to an end. Only remaining last 3 biological entities. For these last 3 biological entities, I will try my best to identify them all, despite my limited biology knowledge. ;)

Enjoy viewing as usual! :)

...to be continued...
Attachments
cb BFandFLuorb.jpg
cb BFandFLuorb.jpg (36.31 KiB) Viewed 9679 times
cb fluoro ph_c1+2.jpg
cb fluoro ph_c1+2.jpg (100.05 KiB) Viewed 9679 times
cb flurob bf_c1+2.jpg
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cb fluro ph_c1+2.jpg8585.jpg
cb fluro ph_c1+2.jpg8585.jpg (176.69 KiB) Viewed 9679 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#14 Post by bkwoo » Thu Oct 08, 2020 6:28 am

Here a bit of information about Malaysians or any other South East Asians (the Thais, the Vietnamese, Indonesians, etc..) and amateur microscopy. ;)

Believe me, people in South East Asia region are not so keen on this type of hobby. Average Malaysians, if they have money to throw in, in order to indulge in a hobby, they will rather spend it on pet dog, cat, birds or fishes in aquarium. Most Malaysians are not so scientifically-inclined to own even a simple basic light microscope, much so spending hours to strain their eyes looking for that amazing microscopic lifeforms. :)

Fishing, in fact, is a far more popular outdoor hobby here among Malaysians. They spend money on expensive fishing rods, accessories and baits from Japan and then go fishing on the riverbank or some remote lakes or even hopping onto a boat to fish somewhere in the middle of South China sea. A much more productive return, they will say because you can cook and eat the fishes that you caught. :lol:

Okay let get back to the pictures.

1st: Nauplius copepod larvae (my identification), BF and FL. Are those the mandibles? :o The hook shape.
2nd: As above, FL merged BF.
3rd: PH.
4th: Cladocera Moina, BF and FL.
5th: Cladocera Moina, BF merged FL.

Enjoy viewing!!

(One last biological entity for me to identify...) :)

.....to be continued.....
Attachments
nauplius BFandFL.jpg
nauplius BFandFL.jpg (22.36 KiB) Viewed 9655 times
nauplius fluoro8585 + ph_c1+2.jpg
nauplius fluoro8585 + ph_c1+2.jpg (143.43 KiB) Viewed 9655 times
nauplius ph.jpg
nauplius ph.jpg (133.95 KiB) Viewed 9655 times
daphnia  BFandFL.jpg
daphnia BFandFL.jpg (25.77 KiB) Viewed 9655 times
daphnia fluoro + bf_c1+2.jpg
daphnia fluoro + bf_c1+2.jpg (68.4 KiB) Viewed 9655 times
Last edited by bkwoo on Sat Oct 10, 2020 7:22 am, edited 1 time in total.
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#15 Post by bkwoo » Sat Oct 10, 2020 3:47 am

Last post for the recent photomicrography session. Last 5 pictures.

1st: Some paramecium or ciliates or just pond protists, (I got this right, right? ;) ) BF and FL.
2nd: Starring the biological samples: Kuala Lumpur's pond sub-milimetre scale creatures (my collection..on left), and the infusoria from a nearby local pet aquarium shop (on right). :)
3rd: Washing and drying steps. Inset: "Kuala Lumpur pond sub-milimetre scale creatures, come and hop aboard!..and say Hello to the world!" (my definition is roughly about 200 to 1000 micron size).
4th: Hardware part, the Zeiss AX10 inverted fluorescence microscope. A very typical university biology lab research-grade fluorescence microscope.
5th: Software part to do the merging BF and FL, etc..ZEN pro 2012.

Thank you all for viewing! :) ;)

....The End.....
Attachments
paramecium BFandFL.jpg
paramecium BFandFL.jpg (25.07 KiB) Viewed 9635 times
protistswhole.jpg
protistswhole.jpg (90.33 KiB) Viewed 9635 times
washingwb.jpg
washingwb.jpg (83 KiB) Viewed 9635 times
zeisswhole.jpg
zeisswhole.jpg (94.85 KiB) Viewed 9635 times
softwarewhole.jpg
softwarewhole.jpg (61.67 KiB) Viewed 9635 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#16 Post by BramHuntingNematodes » Sat Oct 10, 2020 4:37 am

That hand-held lens is nice piece of microscope hardware. Very classy!
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#17 Post by bkwoo » Fri Dec 18, 2020 4:27 am

As the year 2020 draws nearer to end, we have seen strides made by humanity in space explorations such as NASA's OSIRIS-REx, China's Change-5, Japan's Hayabusa 2 space probes despite in the midst of Covid-19 pandemic.

So too shall advances been made in amateur microscopy as permanent fluorescence microscopy slides for amateur microscopy are now a possible reality. ;)

Here are the pictures from the permanent fluorescence microscopy slides that I have made. Enjoy viewing as usual!! :)

First three pictures are cylopoid copepod with and without eggs.
Fourth picture is daphnia pulex? or cladocera moina? :roll:
Fifth picture is cladocera moina.


---------------------------------------to be continued........................................................
Attachments
copepodBBFFL9090.jpg
copepodBBFFL9090.jpg (34.5 KiB) Viewed 9300 times
copepodeggA.jpg
copepodeggA.jpg (36.17 KiB) Viewed 9300 times
copepodeggB.jpg
copepodeggB.jpg (21.31 KiB) Viewed 9300 times
magna.jpg
magna.jpg (28.99 KiB) Viewed 9300 times
moinaA.jpg
moinaA.jpg (33.12 KiB) Viewed 9300 times
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#18 Post by bkwoo » Sat Dec 19, 2020 7:38 am

First: Cladocera Moina

Second: Cyclopoid copepod

Third: Ostracod? :roll: dried up, this is from a temporary slide, we viewed this slide last after viewing six permanent slides, after more than 4 hours since we have it prepared. The wet mountant works.

Fourth: Same dried up ostracod, higher magnification.

Fifth: Two moinas, this is my first permanent slide, my first attempt. I could have sworn that I put in five of these creatures on the glass slide, but once I put the sticky coverslip down, the result surprisingly revealed only two moinas. :lol:

-------------------------to be continued...........................................
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#19 Post by bkwoo » Mon Dec 21, 2020 9:05 am

Merged pictures from previous posting,

-----------------------------------------------to be continued..........................................................
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#20 Post by bkwoo » Tue Dec 22, 2020 8:59 am

Coming to an end.

...........................to be continued............................................
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#21 Post by bkwoo » Wed Dec 23, 2020 1:15 pm

The last post.

First: Dual moinas.

Second: My pond collection has evolved into ostracod culture. Ostracods population just boomed when the water starts to turn murky. Water flea creatures die off leaving only lots and lots of ostracods and copepods. So both ostracods and copepods can co-exist together (this I learnt from dabbling in amateur microscopy!).
Somewhere on this picture, one can see the ostracods' molding leftover?, floating on the water surface. This ostracod sure look like millenium falcon's shape, don't they? :)

Third: All the supporting items for photomicrography session. Black cap bottle to collect and to contain the biological entities, gold cap bottle is the magic ingredient water as wet mount. Glycerin 100%, Nail lacquer or clear nail polish (very sticky stuff). From top : D, B, B, C, X and Y are the six permanent fluorescence microscopy glass slides. The other two: single concave and double concave slides serve as temporary slides. Dual moinas are in slide D.

Fourth: "The Boss" is now contemplating whether she should submit her name as entrant to Nikon Small World photomicrography competition. She has done enough warm-up practice runs, hasn't she? That will be another story ....a new challenge perhaps? :?:

Fifth: Many Malaysian microscopists contributed to the pictures on this thread, without them then this thread is not possible and for that I have to thank them all, whether their effort was successful or not for they have their own varying degree set of skills in handling microscope. Only "The Boss" took up the challenges and thus have come to this far.

This is it, the closing of this chapter (January 2019 to December 2020), my two years experiences in amateur microscopy. ;)

Merry Christmas and Happyy Holiday to all, members and non-members, and stay safe in these pandemic time.



--------------------------------------------------------------------------------The End-------------------------------------------------------------------------------------------------------------
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#22 Post by bkwoo » Fri Jan 22, 2021 9:05 am

A new year and a new approach to presenting the fluorescence pictures with a little bit of help from adobe photoshop.

Old pictures but manipulated. Enjoy viewing! :)
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#23 Post by BramHuntingNematodes » Fri Jan 22, 2021 6:17 pm

As always, very interesting images. Are these investigations being carried out in support of some aquacultural endeavor or is it pure amateur naturalism?
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#24 Post by bkwoo » Sat Jan 23, 2021 2:57 am

More on the amateur hobbyist-artistic pursuit of fluorescence microscopy. Specific staining is more for scientific endeavour. This is not. ;)
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#25 Post by bkwoo » Sat Oct 16, 2021 1:34 am

An old picture. I used photoshop to improve it. Some kind of oil palm microspores (Malaysia is famous for palm oil :lol: ), small venture of fluorescence into plant biology beside the onion epidermal cells. :)

Some new FL pictures from Year 2 Covid-19, the pandemic year continues. Enjoy viewing as usual. Pictures taken from permanent fluorescence microscopy glass slides. ;) :) Pond sub-milimetre creatures as usual.
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#26 Post by bkwoo » Sat Oct 30, 2021 8:15 pm

Some new FL pictures from Year 2 Covid-19, the pandemic year continues. Enjoy viewing as usual. Pictures taken from permanent fluorescence microscopy glass slides. ;) :) Pond sub-milimetre creatures as usual. Well....creature biology excites me more than plant biology. :lol:
The first one looks like "Ghostface" killer from the movie Scream. :lol: :P

Needs to play around with photoshop adobe's brightness, contrast, etc to highlight their internal organs and structures. ;)
Thanks for the compliments.
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#27 Post by PeteM » Sat Oct 30, 2021 9:00 pm

Thanks for these posts.

Many of the images, particularly some with phase and fluorescence, are pretty spectacular.

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Re: The Art of Fluorescence Microscopy (DAPI filter)

#28 Post by bkwoo » Fri Dec 31, 2021 9:00 am

Always two they are, who walk the dark path of the dark side of microscopy....the "Boss" and her assistant, the "Artist". ;)

1st: Moinas in space, FL
2nd: Daphnia magna, DF+FL or ghost white
3rd: Baby brine shrimp, artistic blue (DF+FL)
4th: Baby brine shrimp, artistic green (DF+FL)
5th: From cancer cells (top) to infusoria (below, artist rendition)

Enjoy viewing as usual! and a happy new year to all MicrobeHunter forum members! :)
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Re: The Art of Fluorescence Microscopy (DAPI filter)

#29 Post by Microscopy_is_fun » Fri Dec 31, 2021 1:11 pm

You have a very nicely equipped microscopy lab, and the quality of your images is getting better with every post!

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Re: The Art of Fluorescence Microscopy (DAPI filter)

#30 Post by bkwoo » Fri Dec 31, 2021 1:18 pm

Thank you for your kind comments. I am still learning especially on the image processing part. ;)
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