Salt water embryo.

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Sabatini
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Joined: Tue Nov 12, 2019 3:09 am

Salt water embryo.

#1 Post by Sabatini » Sat Mar 27, 2021 6:28 pm

Hello .
Here is a sample taken from the Caribbean Sea, is some kind of embryo .
The images are comparative for the leitz Phaco 25x and the leitz PV 20x. With the
Heine condenser on the Leitz Ortholux 1
I still can't find the right light settings for the Heine even if I use a white LED...the Heine still gives very yellowish images, like a halogen light...only if I use the blue filter it gets better.
IMG_20210327_130517.jpg
IMG_20210327_130517.jpg (64.67 KiB) Viewed 2557 times
PV 20x
IMG_20210327_130609.jpg
IMG_20210327_130609.jpg (64.26 KiB) Viewed 2557 times
NPL phaco 25x

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Rossf
Posts: 363
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Location: Victoria Australia

Re: Salt water embryo.

#2 Post by Rossf » Mon Mar 29, 2021 11:33 am

Beautiful microbe Sabatini-I’ve got a heine condenser also and yours does seem to have this sort of yellowish chromatic aberration thing going on-especially on defined edges like the scalloping structure around the internal egg-is there any oil that has gotten onto the mirror part where the light ring exits? Might have to look close with a turned around eyepiece.Do you have the immersion top as well? Does it still do this with that on? Or is it on already? Can’t remember how low objective you can use with the top on.If top is on is there dried oil underneath the top element where the glass balloons out? Does the bottom glass element look clean or is it yellowish ? If so maybe oil has gotten down to lower glass elements..just some ideas but mine doesn’t behave like that. Is the Pv 20x the ‘h’ type? They have a blue background a bit like Zeiss phase optics. Hope you can solve this problem. viktor j nilsson may have some ideas as well.hey viktor!
Good luck ross

viktor j nilsson
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Location: Lund, Sweden

Re: Salt water embryo.

#3 Post by viktor j nilsson » Mon Mar 29, 2021 11:43 am

Hey! I do have a Heine, but I've never used it with Leitz Pv objectives. Only Nikon Phase objectives (that have a MUCH thicker phase rings than the light cone produced by the Heine) and as a darkfield condenser. I would have thought that it would perform beautifully with the right objectives, so I am a little surprised to hear about your problems.

Have you tried to actually use it with the correct light unit for your microscope? Preferably halogen? Could it be that it is your LED illuminator that is causing problems? I wouldn't be surprised if the fincky Heine would expose issues with your lighting that were not evident in brightfield.

What are we seeing in your images. First one is with blue filter, second without? What exactly is it that you find objectionable?
The phase effect doesn't seem very strong in either of them. What does the phase rings in the back focal plane look like?

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Rossf
Posts: 363
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Location: Victoria Australia

Re: Salt water embryo.

#4 Post by Rossf » Mon Mar 29, 2021 2:28 pm

Hey viktor j nilsson-I’ve got Pv objectives and they don’t get that weird yellow fringing-first photo is Pv,type ‘h’ I suspect-second is phaco which have thicker ring than the Pv objectives but same problem-also has that fringing-I’ve wondered at times if certain vintage optics perform best with the type of lighting they were designed for-ie tungsten or halogen. I think both photos are with blue filter but Sabatini indicated it only partially helped the issue-hope you didn’t mind me calling you like Tarzan just thought you might have some better ideas.
Regards ross

Sabatini
Posts: 464
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Re: Salt water embryo.

#5 Post by Sabatini » Mon Mar 29, 2021 6:29 pm

Thank you for your valuable suggestions and your interest I will try to follow your instructions as soon as possible.
Thank you Ross for alerting with your call....
At the moment I am not using the inmersión cap as I only use low magnifications , max phaco NPL 40x ,but I will take some pictures with it and without for comparison.
Both images were taken with a blue filter.
I really think that the lighting is the possible cause of the regular images mainly in this case.
I will try using a halogen light with blue filter and will be updating with some images.
The thing is that Heine is a condenser that needs a lot of illumination and almost always my lights fall short to achieve a good result.
I will be motivated by your interest to experiment a bit later.
Thank you !
Last edited by Sabatini on Tue Mar 30, 2021 12:19 am, edited 1 time in total.

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Rossf
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Location: Victoria Australia

Re: Salt water embryo.

#6 Post by Rossf » Mon Mar 29, 2021 7:18 pm

Sabatini just in case you don’t have it here’s a link to the heine system manual. I had a quick look and there describes adjusting lighting for the ortholux is done without the condenser in place at all and only inserting the heine condenser after the lighting is set up properly-it’s a good read anyway-I often go back to the manual for a look-it is fussy with lighting and seems to like things a certain way to perform its best.good luck and if these ideas fixes the issue please post a good photo so other people can benefit if they encounter the same problem👌
Regards Ross

http://microscope.database.free.fr/Acce ... denser.pdf

Sabatini
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Re: Salt water embryo.

#7 Post by Sabatini » Thu Apr 01, 2021 6:16 pm

CramIt_Screenshot_2021-04-01-12-04-25-183_com.google.android.apps.photos20210401_130717.jpg
CramIt_Screenshot_2021-04-01-12-04-25-183_com.google.android.apps.photos20210401_130717.jpg (69.32 KiB) Viewed 2433 times
CramIt_IMG_20210401_12041920210401_130717.jpg
CramIt_IMG_20210401_12041920210401_130717.jpg (87.2 KiB) Viewed 2433 times
leitz PV 20x Heine condenser.
Leitz PV 10x
Leitz PV 10x
CramIt_IMG_20210401_12061920210401_130716.jpg (85.27 KiB) Viewed 2433 times
Leitz Npl phaco 25x
Leitz Npl phaco 25x
CramIt_IMG_20210401_12100920210401_130716.jpg (145.72 KiB) Viewed 2433 times

Sabatini
Posts: 464
Joined: Tue Nov 12, 2019 3:09 am

Re: Salt water embryo.

#8 Post by Sabatini » Thu Apr 01, 2021 6:17 pm

Back focal plane leitz Heine
Back focal plane leitz Heine
IMG_20210401_120720.jpg (27.7 KiB) Viewed 2433 times
IMG_20210401_120727.jpg
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IMG_20210401_120740.jpg
IMG_20210401_120740.jpg (21.84 KiB) Viewed 2433 times

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Rossf
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Location: Victoria Australia

Re: Salt water embryo.

#9 Post by Rossf » Sun Apr 04, 2021 12:40 pm

So Sabatini the later pictures look way better-what did you do differently?-one downfall of the heine condenser is I often find it harder to see the phase ring compared to a turret condenser.
Regards ross

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