Trying out my new objective
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Trying out my new objective
It's a Nikon CF Fluorite F100-UV 100/1.30 glycerine immersion. I tried it with some pollen mounted in glycerine and with glycerine immersion. It seemed to work ok but the images weren't very interesting. I also tried it with glycerine immersion on some diatoms mounted in Pleurax. Not homogenous, but it seemed to still work ok. I guess not optimal, but still.
So some diatoms
Cropped and resized to 77%
Cropped and resized to 50%
Cropped and resized to 61%
Cropped and resized to 41%
All taken with my Gpcam3 178M at 415nm on my Swift380T
Louise
So some diatoms
Cropped and resized to 77%
Cropped and resized to 50%
Cropped and resized to 61%
Cropped and resized to 41%
All taken with my Gpcam3 178M at 415nm on my Swift380T
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
Very lovely high resolution images.
Once again demonstrate immersion power.
Oblique, aren't they ? how did you get the oblique illumination ?
Since that is a Nikon Cf, would be interesting to see color images as well.
Incidentally, is that a Fluorite objective ? the CF and F do not mean fluorite in this case, I think.
Once again demonstrate immersion power.
Oblique, aren't they ? how did you get the oblique illumination ?
Since that is a Nikon Cf, would be interesting to see color images as well.
Incidentally, is that a Fluorite objective ? the CF and F do not mean fluorite in this case, I think.
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Re: Trying out my new objective
Thanks! Yeah, I was a bit worried that the glycerine immersion might mess up the Pleurax mount images completely but I think they are acceptable. I've ordered some borosilicate 3.3 coverslips and will see if they make things better - or worse! (with my own slides).
I'm using an LED mounted on a heatsink under the condenser so I can move it it around to get oblique illumination.
I'll try it with the dslr. Also the 40/0.85 Fluor I recently bought. I've been carried away with the mono imaging!
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
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Re: Trying out my new objective
Comparing the Fluorite Petroneis with one taken by a 100x oil achromat. The image is much reduced and I rotated the achromat one to be about the same orientation as the Nikon Fluorite:
Louise
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
Getting better all the time !
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Re: Trying out my new objective
That was just a quick comparison using the previous Petroneis image I took. You'd expect the Nikon fluorite to be better than the Swift achromat! Still, I think the Nikon 100x was a bargain! Got it from the same Japanese seller as the Nikon 40/0.85 fluor. Both good, and both good value for money The 100x was actually a lot cheaper than the 40x!
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
Lovely image from that FluoriteLouiseScot wrote: ↑Sun Apr 11, 2021 5:52 pmPetroneis_100xAchro_vsFluorCrop50pc_Red0017.jpg
.
Louise
... but what's with the 'staircase' effect [very evident at upper left] ?
MichaelG.
Too many 'projects'
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Re: Trying out my new objective
Very impressive. Do you find that colors seem to wash out at high magnification?
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Re: Trying out my new objective
They are monochrome images taken with a monochrome astro camera with illumination from a 415nm (blue-violet) , 3W LEDDonSchaeffer wrote: ↑Sun Apr 11, 2021 6:30 pmVery impressive. Do you find that colors seem to wash out at high magnification?
I will take some colour ones with the dslr next time.
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
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Re: Trying out my new objective
Thanks. Not sure what you mean re 'staircase??MichaelG. wrote: ↑Sun Apr 11, 2021 6:26 pmLovely image from that FluoriteLouiseScot wrote: ↑Sun Apr 11, 2021 5:52 pmPetroneis_100xAchro_vsFluorCrop50pc_Red0017.jpg
.
Louise
... but what's with the 'staircase' effect [very evident at upper left] ?
MichaelG.
ps Oh think I see - not sure. May be the oblique illumination else, possibly, refraction effects because of the mismatch between the glycerine, glass and mounting medium. I only got the objective yesterday and will have to try it out in different situations. I didn't notice the same anomaly in the other images above.
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
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Re: Trying out my new objective
This is a different individual I imaged from the same slide at more or less the same time:LouiseScot wrote: ↑Sun Apr 11, 2021 6:37 pmThanks. Not sure what you mean re 'staircase??MichaelG. wrote: ↑Sun Apr 11, 2021 6:26 pmLovely image from that FluoriteLouiseScot wrote: ↑Sun Apr 11, 2021 5:52 pmPetroneis_100xAchro_vsFluorCrop50pc_Red0017.jpg
.
Louise
... but what's with the 'staircase' effect [very evident at upper left] ?
MichaelG.
ps Oh think I see - not sure. May be the oblique illumination else, possibly, refraction effects because of the mismatch between the glycerine, glass and mounting medium. I only got the objective yesterday and will have to try it out in different situations. I didn't notice the same anomaly in the other images above.
Louise
It shows the same effect. It looks like it's to do with that species- maybe they are quite deep?
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
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Re: Trying out my new objective
Before I took stills (selection above) I made a short .avi of a diatom on another slide. I recorded for 2 1/2 mins but it only plays for 18 secs. Must be a setting... I should have changed the size/dimensions too so it was the full 3040x2048... Next time I'll try harder to get it right It's unedited.
https://youtu.be/eiEKgfmMj_4
Of course you can pause it and inspect at any point
Louise
https://youtu.be/eiEKgfmMj_4
Of course you can pause it and inspect at any point
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
.LouiseScot wrote: ↑Sun Apr 11, 2021 7:05 pmIt shows the same effect. It looks like it's to do with that species- maybe they are quite deep?
I’ve just found this:
https://www.researchgate.net/publicatio ... _do_Brasil
Have a look at Figure 3 ... it does seem to show a similar effect
Interesting !!
MichaelG.
Too many 'projects'
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Re: Trying out my new objective
I'm sure there's a simple explanation...MichaelG. wrote: ↑Mon Apr 12, 2021 8:46 am.LouiseScot wrote: ↑Sun Apr 11, 2021 7:05 pmIt shows the same effect. It looks like it's to do with that species- maybe they are quite deep?
I’ve just found this:
https://www.researchgate.net/publicatio ... _do_Brasil
Have a look at Figure 3 ... it does seem to show a similar effect
Interesting !!
MichaelG.
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
Hey LouiseScot your doing a great job of upgrading the Swift-your images just keep getting better and you seem to really know what you want in an objective-thats why I stuck with finite systems-I like to “shop” for optics from a variety of makers-I got a few good objectives from Japanese sellers on eBay recently as well-your enthusiasm is infectious.
Regards ross
Regards ross
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Re: Trying out my new objective
Thanks, Ross! Well I just went with the Nikon cf range which don't need any extra correction and, of course, they are an excellent make, as well as being parfocal. Coming across the two fluors recently was good luck, I think! As I mentioned, the fluorite glycerine immersion lens was a real bargain. I also have a diy infinity system based on a macro focus rail but I've rather neglected it lately. I will do something useful with it soon! So much to do, so little time!Rossf wrote: ↑Mon Apr 12, 2021 9:22 amHey LouiseScot your doing a great job of upgrading the Swift-your images just keep getting better and you seem to really know what you want in an objective-thats why I stuck with finite systems-I like to “shop” for optics from a variety of makers-I got a few good objectives from Japanese sellers on eBay recently as well-your enthusiasm is infectious.
Regards ross
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
I think most of us here a guilty of neglecting a piece of gear for a while..especially while enthusiastically going down a particular rabbit hole! But it’s always good to reconnect with that different scope down the road-I think that helps keep enthusiasm going sometimes..just taking a side step to another optical adventure-I’ve got a sneaking suspicion your fascination with the optical effects of glass goes back to childhood-mine did! I was always looking through glass-I grew up in the 70’s so had lovely chunky ashtrays etc to observe how light behaves as it goes through-playing with family movie cameras and filters(!)-they seemed magic at 8 years old! Well still do...
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Re: Trying out my new objective
I'm a child of the 50's My first microscope arrived when I persuaded my mum to get one on special offer on a cereal packet. Can't remember which one - think it was Shredded Wheat. It was a plasticky thing but quite good, nevertheless. It was the first time I was able to see protozoa and such Then when I was 18 I left school and went to work in a big pharma quality control / bacteriology lab. I used to collect various environmental samples and make agar plates, often with added horse blood. Make slides, Gram stain them and examine them under a microscope. I loved it! But moved into biochemistry a bit later. More recently (2009-13) I was doing research into a Rett Syndrome (a genetic disease) and was doing patch clamping of mouse brain slices using water immersion/IR-DIC, and further examination of antibody staining with a laser scanning confocal microscope. Got my name on a paper too I'm retired now. I have several refractor telescopes also but the weather and sky conditions here are not really conducive, so I've mostly moved back to microscopes - back to where I started Oddly enough, through all the time I've been using microscopes it's only since I've been a member here that I've started delving into the deeper aspects of microscopes and objectives, and lighting etc. etc. There's always more to learn!Rossf wrote: ↑Mon Apr 12, 2021 9:49 amI think most of us here a guilty of neglecting a piece of gear for a while..especially while enthusiastically going down a particular rabbit hole! But it’s always good to reconnect with that different scope down the road-I think that helps keep enthusiasm going sometimes..just taking a side step to another optical adventure-I’ve got a sneaking suspicion your fascination with the optical effects of glass goes back to childhood-mine did! I was always looking through glass-I grew up in the 70’s so had lovely chunky ashtrays etc to observe how light behaves as it goes through-playing with family movie cameras and filters(!)-they seemed magic at 8 years old! Well still do...
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
The thickness of the diatom is being measured right now...LouiseScot wrote: ↑Mon Apr 12, 2021 9:05 amI'm sure there's a simple explanation...MichaelG. wrote: ↑Mon Apr 12, 2021 8:46 am.LouiseScot wrote: ↑Sun Apr 11, 2021 7:05 pmIt shows the same effect. It looks like it's to do with that species- maybe they are quite deep?
I’ve just found this:
https://www.researchgate.net/publicatio ... _do_Brasil
Have a look at Figure 3 ... it does seem to show a similar effect
Interesting !!
MichaelG.
Louise
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Re: Trying out my new objective
Says 30um here http://nordicmicroalgae.org/taxon/Petroneis%20humerosaHobbyst46 wrote: ↑Mon Apr 12, 2021 1:32 pmThe thickness of the diatom is being measured right now...LouiseScot wrote: ↑Mon Apr 12, 2021 9:05 amI'm sure there's a simple explanation...MichaelG. wrote: ↑Mon Apr 12, 2021 8:46 am
.
I’ve just found this:
https://www.researchgate.net/publicatio ... _do_Brasil
Have a look at Figure 3 ... it does seem to show a similar effect
Interesting !!
MichaelG.
Louise
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
Thickness - neither width nor height.
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Re: Trying out my new objective
? thickness = height
The source gives length, width and height - good enough?
A 'thickness' of 30um seems quite a lot when width is also 30um
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
I stand corrected, sorry !LouiseScot wrote:The source gives length, width and height - good enough?A 'thickness' of 30um seems quite a lot when width is also 30um
So here is the measurement. Recorded with the 16x0.32 Plan objective.
Photo 1 shows the LED strip stand. Laid on the microscope stage (usually the stereo microscope, but for the present purpose - on the compound). The slide holder arm is taped to the stage, to keep it away. The LED strip is vertical, and the blackened slide is laid on the stage beneath the LEDs.
The blackened slide is, in fact, a strew slide of gently cleaned diatoms (photo 2). From this Smaug's treasure heap I peak the gem, and place it in a quiet vacant dark area (photo 3). I lies vertically, almost, slanted just a bit, the apex points up (photo 4). The stage micrometer (photo 5) imaged at the same conditions provide the measurement scale.
The length of the diatom is estimated on the basis of its siblings (one of them is visible in photo 2) to be 60-65um.
The width is not reliable, since the diatom is slanted.
The thickness, according to photos 3 and 4, is estimated to be ~15 um. Could be more, though. I have to improve the illumination for better accuracy.
The gentle cleaning can preserve the complete frustule.
So these are complete frustules. This conclusion is somewhat supported by the fact, that when an inappropriate mountant was used - Norland 61 adhesive rather than Pleurax - large air bubbles stayed within this very frustule.
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- 1) LED stand for handling dry diatoms on a blackened slide.jpg (105.78 KiB) Viewed 5489 times
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- 2) Diatom keeper black slide 2.JPG (169.38 KiB) Viewed 5489 times
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- 3) Resized diatom.jpg (47.13 KiB) Viewed 5489 times
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- 4) Cropped diatom.jpg (55.92 KiB) Viewed 5489 times
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- 5) Stage micrometer view under 16X objective. Line spacing = 10 um.JPG (66.87 KiB) Viewed 5489 times
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Re: Trying out my new objective
Wow! So many you have their Bilbo! So is that actually a Petroneis sideways on? I'd say it was more like 20um just measuring with a ruler on screen. So 20x60 isn't far removed from 30x70. Anyway, I'm still not sure of the causes of the image aberrations above...Hobbyst46 wrote: ↑Mon Apr 12, 2021 2:40 pmI stand corrected, sorry !LouiseScot wrote:The source gives length, width and height - good enough?A 'thickness' of 30um seems quite a lot when width is also 30um
So here is the measurement. Recorded with the 16x0.32 Plan objective.
Photo 1 shows the LED strip stand. Laid on the microscope stage (usually the stereo microscope, but for the present purpose - on the compound). The slide holder arm is taped to the stage, to keep it away. The LED strip is vertical, and the blackened slide is laid on the stage beneath the LEDs.
The blackened slide is, in fact, a strew slide of gently cleaned diatoms (photo 2). From this Smaug's treasure heap I peak the gem, and place it in a quiet vacant dark area (photo 3). I lies vertically, almost, slanted just a bit, the apex points up (photo 4). The stage micrometer (photo 5) imaged at the same conditions provide the measurement scale.
The length of the diatom is estimated on the basis of its siblings (one of them is visible in photo 2) to be 60-65um.
The width is not reliable, since the diatom is slanted.
The thickness, according to photos 3 and 4, is estimated to be ~15 um. Could be more, though. I have to improve the illumination for better accuracy.
The gentle cleaning can preserve the complete frustule.
So these are complete frustules. This conclusion is somewhat supported by the fact, that when an inappropriate mountant was used - Norland 61 adhesive rather than Pleurax - large air bubbles stayed within this very frustule.
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Trying out my new objective
Yes, it is sideways on. I flipped it over with the tip of the glass fiber then back on its top. The exact thickness is really an estimate. Since it was not exactly perpendicular to the plane of the slide.LouiseScot wrote: ↑Mon Apr 12, 2021 3:36 pmWow! So many you have their Bilbo! So is that actually a Petroneis sideways on? I'd say it was more like 20um just measuring with a ruler on screen. So 20x60 isn't far removed from 30x70. Anyway, I'm still not sure of the causes of the image aberrations above...
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Re: Trying out my new objective
I couldn't do that! My hands would probably shake too much!Hobbyst46 wrote: ↑Mon Apr 12, 2021 3:43 pmYes, it is sideways on. I flipped it over with the tip of the glass fiber then back on its top. The exact thickness is really an estimate. Since it was not exactly perpendicular to the plane of the slide.LouiseScot wrote: ↑Mon Apr 12, 2021 3:36 pmWow! So many you have their Bilbo! So is that actually a Petroneis sideways on? I'd say it was more like 20um just measuring with a ruler on screen. So 20x60 isn't far removed from 30x70. Anyway, I'm still not sure of the causes of the image aberrations above...
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo