amoeba of the day..

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chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

amoeba of the day..

#1 Post by chrisimbee » Tue Apr 20, 2021 2:59 pm

hello,

just a fex pic of some amoebas found in one of my petri dish...
20 avril 21 amibe 3.jpg
20 avril 21 amibe 3.jpg (107 KiB) Viewed 1479 times
20 avril 21 amibe 3.jpg
20 avril 21 amibe 3.jpg (107 KiB) Viewed 1479 times
Microscope Bresser trino research,
phase contrast objectif LOMO x40
daylight filter
astro camera : ASI ZWO 120MC
Attachments
20 avril 21 amibe 2.jpg
20 avril 21 amibe 2.jpg (127.36 KiB) Viewed 1479 times
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: amoeba of the day..

#2 Post by chrisimbee » Tue Apr 20, 2021 3:00 pm

there must be a bugg somewhere.. i posted the 1st pic only once and it posted it twice ;
it did that to me yesterday too..

Chris
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

User avatar
IanW
Posts: 289
Joined: Sat Mar 07, 2015 2:58 pm
Location: Lancashire England

Re: amoeba of the day..

#3 Post by IanW » Tue Apr 20, 2021 8:12 pm

Very good phase contrast effect. Which particular Lomo objective did you use?
Zeiss Jena NF, Zeiss Standard 18 and WL

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: amoeba of the day..

#4 Post by chrisimbee » Tue Apr 20, 2021 9:31 pm

Thanks a lot ; I used a

LOMO x40 phase

and a last photo without the blue filter ; so far i dont have any phase condenser : i've just drawn a phase annulus on the frosted filter and put it into the filter holder of the condenser ; and adjusted the height in order to get superimposed both the image of the annulus with the phase plate .
20 avril 21 amibe 4.jpg
20 avril 21 amibe 4.jpg (65.93 KiB) Viewed 1445 times
Attachments
20 avril 21 lomox20 phase plate.jpg
20 avril 21 lomox20 phase plate.jpg (74.57 KiB) Viewed 1445 times
20 avril 21 LOMO x 20 Ph+ de face.jpg
20 avril 21 LOMO x 20 Ph+ de face.jpg (73.19 KiB) Viewed 1445 times
Last edited by chrisimbee on Tue Apr 20, 2021 9:34 pm, edited 1 time in total.
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: amoeba of the day..

#5 Post by chrisimbee » Tue Apr 20, 2021 9:32 pm

20 avril 21 centrage anneau excentré.jpg
20 avril 21 centrage anneau excentré.jpg (73.3 KiB) Viewed 1445 times
20 avril 21 centrage 3.jpg
20 avril 21 centrage 3.jpg (44.15 KiB) Viewed 1445 times
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: amoeba of the day..

#6 Post by chrisimbee » Tue Apr 20, 2021 9:37 pm

sorry i made a mistake with the photos (i posted the new LOMO phase x20 i received last we) .
obj LOMO ph x40 b .jpg
obj LOMO ph x40 b .jpg (121.63 KiB) Viewed 1445 times
obj LOMO ph x40.jpg
obj LOMO ph x40.jpg (113.36 KiB) Viewed 1445 times
they are still available on ebay and my microscope cant work on Köhler illumination and nevertheless I can make the phase objectives work fine !
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

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