melosira + pleurosigma
melosira + pleurosigma
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Zeiss Planapo 63x/1.4, DIC, 20µm width (melosira), stacked in Photoshop, Marine sample, Alboran Sea.
I prefer it when they are on their own, this is messy, still, thought it worth a stack. In this case 54 images.
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Zeiss Planapo 63x/1.4, DIC, 20µm width (melosira), stacked in Photoshop, Marine sample, Alboran Sea.
I prefer it when they are on their own, this is messy, still, thought it worth a stack. In this case 54 images.
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- melosira + pleurosigma-.jpg (235.89 KiB) Viewed 3143 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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- Posts: 1167
- Joined: Tue Jul 07, 2020 1:51 pm
- Location: Scotland
Re: melosira + pleurosigma
Lovely! I'm intrigued to know how you manage to stack live subjects - I mean, aren't things moving around a lot?
Louise
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: melosira + pleurosigma
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Thanks LouiseScot
I pin them with the cover slip. To do this i wick the water from under the cover slip using absorbent material - usually tissue paper.
This has two functions, it holds the subject down and places it in the 'sweet spot' re imaging.*
This does not always work of course, even the smallest piece of detritus can stop the cover slip from pinning the subject.
One can also move things around in hopes of getting a better arrangement by adding water and then removing it.
One adds water by placing a drop on the edge of the cover slip - capillary action does the rest.
*(The layer of water between the subject and the bottom of the cover slip effectively adds to the cover slip thickness.)
Thanks LouiseScot
I pin them with the cover slip. To do this i wick the water from under the cover slip using absorbent material - usually tissue paper.
This has two functions, it holds the subject down and places it in the 'sweet spot' re imaging.*
This does not always work of course, even the smallest piece of detritus can stop the cover slip from pinning the subject.
One can also move things around in hopes of getting a better arrangement by adding water and then removing it.
One adds water by placing a drop on the edge of the cover slip - capillary action does the rest.
*(The layer of water between the subject and the bottom of the cover slip effectively adds to the cover slip thickness.)
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: melosira + pleurosigma
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Thanks Luis Carlos, most kind!
Thanks Luis Carlos, most kind!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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- Posts: 105
- Joined: Fri Mar 19, 2021 5:34 pm
Re: melosira + pleurosigma
amazing
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100
-
- Posts: 1167
- Joined: Tue Jul 07, 2020 1:51 pm
- Location: Scotland
Re: melosira + pleurosigma
Ahh right - ok, thanks!75RR wrote: ↑Sat May 08, 2021 5:30 am.
Thanks LouiseScot
I pin them with the cover slip. To do this i wick the water from under the cover slip using absorbent material - usually tissue paper.
This has two functions, it holds the subject down and places it in the 'sweet spot' re imaging.*
This does not always work of course, even the smallest piece of detritus can stop the cover slip from pinning the subject.
One can also move things around in hopes of getting a better arrangement by adding water and then removing it.
One adds water by placing a drop on the edge of the cover slip - capillary action does the rest.
*(The layer of water between the subject and the bottom of the cover slip effectively adds to the cover slip thickness.)
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: melosira + pleurosigma
Count me in as thoroughly admiring that shot.
To me, capturing two side by side like that is 4x the challenge and 4x the excellence when done so well -- compared to equally excellent individual shots.
To me, capturing two side by side like that is 4x the challenge and 4x the excellence when done so well -- compared to equally excellent individual shots.
Re: melosira + pleurosigma
Indeed, a perfectly lovely image!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: melosira + pleurosigma
Another Very Nice Image.
Re: melosira + pleurosigma
The 63x1.4 must be rescued and returned to use, for us to see the lovely images !
Re: melosira + pleurosigma
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Many thanks everyone - most kind!
Many thanks everyone - most kind!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)