Polarized live Diatom (Pleurosigma sp)
Polarized live Diatom (Pleurosigma sp)
Hi,
In the last few weeks I could not expend much time with scope and when I did I did not manage to get results worth sharing.
A new stack, adapting to the microscope is proving to be quite challenging. Preparing the samples and working with live moving subjects.
It took me several times until I could complete a stack of this Diatom (ID?) from a marine water sample, it reacted a little to polarized light so I run this one. 22 shots with nikon CFN Planapo 40/0.95 160/0.17; direct projection plus 50% crop with Sony A5100
Full size
Until now with every diatom (or almost anything else) I have tried to run a stack with I had to retouch from individual frames, no way I can get a clean stack straight on. Deciding what details to take from each frame is difficult as I do not fully understand yet what I am really looking at and which details are important to keep.
Also a short BF video I made
One more; this time BF with same lens, stack from 21 images
Full size
A different polarized stack, this time I left the backgound blue, which seems to suit it better than trying to fix the WB
Stack from 18 images, same objective.
I made two versions; first like before showing internal details
Full size
and one trying to keep only superficial texture, more translucent like. There seems to be some banding, will try to fix it
Full size
In the last few weeks I could not expend much time with scope and when I did I did not manage to get results worth sharing.
A new stack, adapting to the microscope is proving to be quite challenging. Preparing the samples and working with live moving subjects.
It took me several times until I could complete a stack of this Diatom (ID?) from a marine water sample, it reacted a little to polarized light so I run this one. 22 shots with nikon CFN Planapo 40/0.95 160/0.17; direct projection plus 50% crop with Sony A5100
Full size
Until now with every diatom (or almost anything else) I have tried to run a stack with I had to retouch from individual frames, no way I can get a clean stack straight on. Deciding what details to take from each frame is difficult as I do not fully understand yet what I am really looking at and which details are important to keep.
Also a short BF video I made
One more; this time BF with same lens, stack from 21 images
Full size
A different polarized stack, this time I left the backgound blue, which seems to suit it better than trying to fix the WB
Stack from 18 images, same objective.
I made two versions; first like before showing internal details
Full size
and one trying to keep only superficial texture, more translucent like. There seems to be some banding, will try to fix it
Full size
Last edited by Seta on Mon May 10, 2021 1:56 pm, edited 4 times in total.
Re: Polarized live Diatom
Wonderful image, and it looks like a Pleurosigma species.
Would be nice to know the appearance without polarization, since the color of the diatom organic parts is green and/or orange/yellow.
Would be nice to know the appearance without polarization, since the color of the diatom organic parts is green and/or orange/yellow.
Re: Polarized live Diatom
Thanks, I added a short video link that may help with the ID, also run a BF stack I have to work with it, but the diatom had broke by then under the coverslip pressure
Re: Polarized live Diatom
Nice video. I tentatively identified from the pattern of dots in the polarized image.
From my experience of handling individual Pleurosima frustules, they are very fragile diatoms.
Re: Polarized live Diatom (Pleurosigma sp)
Agree with other comments above - wonderful clean and high resolution photographs.
Zeiss Jena NF, Zeiss Standard 18 and WL
Re: Polarized live Diatom (Pleurosigma sp)
Getting a clean stack with this high NA lenses needs lots of retouching; being an APO lens does help a lot.
Re: Polarized live Diatom (Pleurosigma sp)
Beautiful pictures nice clean backgrounds. Kudos
RB
RB
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Re: Polarized live Diatom (Pleurosigma sp)
nice work!