rotifer in acridin orange under blue light

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chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

rotifer in acridin orange under blue light

#1 Post by chrisimbee » Wed May 12, 2021 6:37 am

Hello,

I bought some acridin orange on ebay ( approx 6E for 20g)!! and just tried it lst night on living protozoa with my BH2 epifluo attachment lit with a simple blue led light bulb 5W.

Once my la room is dark its easier to see the faint fluorescence live but as the astro camera I use is highly sensitive its much easier. I'm working on making a much powerflull blue light source (@ 405nm or rather @ 460-470 nm with cob led).

As the fluorescence isnt very strong yet the exposure time doent allow to capture the nicely tainted paramacia that run too fast but still I was able to get this rotifer and another very still protist...

Enjoy..

https://www.youtube.com/watch?v=LKOJ7_P ... chrisimbee

https://www.youtube.com/watch?v=XyC7fVp_Ukw
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

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