Hello,
I bought some acridin orange on ebay ( approx 6E for 20g)!! and just tried it lst night on living protozoa with my BH2 epifluo attachment lit with a simple blue led light bulb 5W.
Once my la room is dark its easier to see the faint fluorescence live but as the astro camera I use is highly sensitive its much easier. I'm working on making a much powerflull blue light source (@ 405nm or rather @ 460-470 nm with cob led).
As the fluorescence isnt very strong yet the exposure time doent allow to capture the nicely tainted paramacia that run too fast but still I was able to get this rotifer and another very still protist...
Enjoy..
https://www.youtube.com/watch?v=LKOJ7_P ... chrisimbee
https://www.youtube.com/watch?v=XyC7fVp_Ukw
rotifer in acridin orange under blue light
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rotifer in acridin orange under blue light
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100