Paramecia that glows...

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chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Paramecia that glows...

#1 Post by chrisimbee » Fri May 14, 2021 9:11 pm

Hello,

today I received a led torch that emmits at 470 nm (blue light) and it matches perfectly the acridin orange spectrum ; so I could use this torch as a source for my epifluorescence module.

I made a slide with some paramecia and a tiny bit of acridin orange..

Here are some photos..
14 mai paramecia.jpg
14 mai paramecia.jpg (46.48 KiB) Viewed 1335 times
pamaramecia 2.jpg
pamaramecia 2.jpg (62.5 KiB) Viewed 1335 times
paramecia 3.jpg
paramecia 3.jpg (54.92 KiB) Viewed 1335 times
paramecia 5.jpg
paramecia 5.jpg (108.37 KiB) Viewed 1335 times
Chris
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

chrisimbee
Posts: 105
Joined: Fri Mar 19, 2021 5:34 pm

Re: Paramecia that glows...

#2 Post by chrisimbee » Fri May 14, 2021 9:13 pm

proto.jpg
proto.jpg (52.36 KiB) Viewed 1334 times
(this one was dead...)
microscope Olympus BH2-BHTU+epifluo RFC @ 470 nm
Zeiss neofluar x16Ph, x40Ph, x100 oilPh
LOMO Ph x10 X20 X40 X90oil
Olympus SPFl x2
Olympus SPx20, SPx40 SPx100
camera astro ZWO ASI 120MM (n&b) et ZWO ASI 120 MC (colour)
Nikon D3100

deBult
Posts: 402
Joined: Tue Jul 16, 2019 3:20 pm
Location: Continental Europe

Re: Paramecia that glows...

#3 Post by deBult » Sat May 15, 2021 4:30 pm

Love image nr 4 (with nr 5) atmosphere, the added material around the ciliate does the trick for me,

Thanks for sharing,

deBult

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Wes
Posts: 1027
Joined: Sat Mar 09, 2019 12:58 pm

Re: Paramecia that glows...

#4 Post by Wes » Sat May 15, 2021 6:28 pm

Awesome. #3 I like the most ;)
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel

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