germinating fern spores

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iconoclastica
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germinating fern spores

#1 Post by iconoclastica » Wed Jul 28, 2021 8:19 pm

The slide already bore an overdose of dry spores used for previous inspection. Then I kept it for about a week in a petri dish with a layer of water.
Dryopteris wallichiana germinating spores 40x
Dryopteris wallichiana germinating spores 40x
kieming3.jpg (79.55 KiB) Viewed 2425 times

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mrsonchus
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Re: germinating fern spores

#2 Post by mrsonchus » Wed Jul 28, 2021 8:39 pm

Excellent - any more images?
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perrywespa
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Re: germinating fern spores

#3 Post by perrywespa » Wed Jul 28, 2021 9:13 pm

Very nice image. Thanks for sharing.
Perry
Insatiably curious.

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iconoclastica
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Re: germinating fern spores

#4 Post by iconoclastica » Thu Jul 29, 2021 9:08 am

mrsonchus wrote:
Wed Jul 28, 2021 8:39 pm
Excellent - any more images?
I am trying to get the spores to develop further, but sofar I only find more of them in this protonemal stage. I just added some nutrients hoping that will make them grow on.

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ImperatorRex
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Re: germinating fern spores

#5 Post by ImperatorRex » Thu Jul 29, 2021 3:42 pm

Really great, without any doubt! Thanks for sharing...
Pls. be welcomed to provide more ;-)

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iconoclastica
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Re: germinating fern spores

#6 Post by iconoclastica » Tue Nov 02, 2021 6:33 pm

ImperatorRex wrote:
Thu Jul 29, 2021 3:42 pm
Really great, without any doubt! Thanks for sharing...
Pls. be welcomed to provide more ;-)
They definitely grow much slower in the petri dish than on earthy media where they already developed small sporophytes. I doesn't help, I suppose, that there are hardly any nutrients available. For a long time they remained uniserial (all the cells in a single row), but now several are making lateral divisions too. This results in an elongated triangular shape with the spore-rest at the top, foreboding the typical heart-shaped form of the gametophyte. Perhaps the low light made them longer then usual?
Dryopteris wallichiana later development stage 20x
Dryopteris wallichiana later development stage 20x
prothallus3.jpg (63.25 KiB) Viewed 1951 times
I found one that's already 3-4 cells wide:
Dryopteris wallichiana later development stage (a bit taller) 20x
Dryopteris wallichiana later development stage (a bit taller) 20x
prothallus2.jpg (70.18 KiB) Viewed 1951 times


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