I was experimenting today with my Ebay dark field adapter and patch set. In the process, I discovered that at 400x one of the smaller patches produced an interesting "gray field" effect. Here's an example;
https://i.imgur.com/7UFOkaI.jpg
Not quite dark field
Re: Not quite dark field
Great Paramecium image. Looks like phase contrast, but since produced with a too small darkfield stop, it is probably Circular Oblique illumination.
Some of the threads here on MicrobeHunter link to very good articles at Micscape:
http://www.microscopy-uk.org.uk/mag/ind ... pjcol.html
Another evaluation is at this academic article:
https://www.spiedigitallibrary.org/jour ... full?SSO=1
Some of the threads here on MicrobeHunter link to very good articles at Micscape:
http://www.microscopy-uk.org.uk/mag/ind ... pjcol.html
Another evaluation is at this academic article:
https://www.spiedigitallibrary.org/jour ... full?SSO=1