urostyla Self-detonation (polarization and dark field)
urostyla Self-detonation (polarization and dark field)
Last edited by woyjwjl on Tue May 03, 2022 3:53 am, edited 2 times in total.
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Re: urostyla Self-detonation (polarization and dark field)
Nice film! Very atmospheric.
The hypotrich ciliates you're calling Urostyla are not in that genus. The first one appears to be Tachysoma pellionellum. I can't identify the chubbier one that appears later (darkfield makes ciliate identification more difficult), however it is probably in the family Oxytrichidae.
The hypotrich ciliates you're calling Urostyla are not in that genus. The first one appears to be Tachysoma pellionellum. I can't identify the chubbier one that appears later (darkfield makes ciliate identification more difficult), however it is probably in the family Oxytrichidae.
Last edited by Bruce Taylor on Tue May 03, 2022 3:49 pm, edited 2 times in total.
Re: urostyla Self-detonation (polarization and dark field)
Thank you for the praise
I only found this at NCBI
Where can I find the corresponding images?
I only found this at NCBI
Where can I find the corresponding images?
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Re: urostyla Self-detonation (polarization and dark field)
Hypotrich ciliates can be difficult to identify, but the common species Tachysoma pellionellum is easier than most, because of its distinctive size and shape (the cell is <100 μm, narrow, often with a distinct lateral bulge in the vicinity of the contractile vacuole, at mid-body), combined with its very long, trailing transverse cirri (usually splayed out and rather stiff). It resembles another common ciliate, Holosticha pullaster, but the latter has its contractile vacuole in the posterior third of the cell (a very unusual position for hypotrichs). T. pellionellum also has long dorsal cilia, which extend around the margin of the cell, as a series of regularly-spaced fine bristles. See pp. 433 ff., here: https://www.researchgate.net/publicatio ... ypotrichia A search in Google Images should turn up some images and videos (but most have not been identified by experts, so be wary). You can also find images of the critter on iNaturalist: https://www.inaturalist.org/home. Older sources may use the name Oxytricha pellionella, and it also sometimes appears under the name Tachysoma pellionella.
The ciliate we see at 2:54 in your video has trailing transverse cirri, and CV is at mid-body (with a distinct swelling in that region). We don't see dorsal cilia (darkfield looks pretty, but not very good for ciliate identification!), but I am pretty sure the critter in that part of the video is T. pellionellum. The one that appears at 6:25 looks quite different, and I can't say much about it, except that it is probably an oxytrichid, and not a urostyloid.
The ciliate we see at 2:54 in your video has trailing transverse cirri, and CV is at mid-body (with a distinct swelling in that region). We don't see dorsal cilia (darkfield looks pretty, but not very good for ciliate identification!), but I am pretty sure the critter in that part of the video is T. pellionellum. The one that appears at 6:25 looks quite different, and I can't say much about it, except that it is probably an oxytrichid, and not a urostyloid.
Re: urostyla Self-detonation (polarization and dark field)
To be honest, these are a bit difficult for a civil engineer
I took the video again yesterday, help me see if this is urostyla?
Then, my phase difference kit will be available next week, maybe improving the shooting is what I like ....
I took the video again yesterday, help me see if this is urostyla?
Then, my phase difference kit will be available next week, maybe improving the shooting is what I like ....
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Re: urostyla Self-detonation (polarization and dark field)
That one is in the Stylonychia mytilus species complex (the cell shape and long caudal cirri are very distinctive). It is also in the family Oxytrichidae.
Phase can resolve certain ciliate features, but good old bright field is often better. DIC is best, of course.
There are many thousands of ciliate species, so yes....identification can be difficult. Don't be discouraged, though. It does get easier, with time.
Phase can resolve certain ciliate features, but good old bright field is often better. DIC is best, of course.
There are many thousands of ciliate species, so yes....identification can be difficult. Don't be discouraged, though. It does get easier, with time.
Re: urostyla Self-detonation (polarization and dark field)
Thanks for the encouragement, maybe this new hobby of mine will last after I turn 50
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