Couple of diatom slides using 405nm light

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JMC
Posts: 57
Joined: Wed Mar 30, 2022 9:21 am

Couple of diatom slides using 405nm light

#1 Post by JMC » Fri May 06, 2022 10:50 am

There is something very beautiful about diatom structures so I try and keep an eye out for diatom slides on ebay. I had a couple turn up this week - a 7 form Test Slide from Klaus Kemp and one antique one by John Barnett with a group of 4 Eupodiscus Radiatus diatoms. These are some quick images of the slides taken using 405nm light, in low magnification (Olympus Splan 20x) and high magnification of a smaller part of each slide (Olympus 60x Splan Apo).

Smaller magnification images.
DSC_9473 mod lab.jpg
DSC_9473 mod lab.jpg (88.12 KiB) Viewed 1308 times
DSC_9471 mod lab.jpg
DSC_9471 mod lab.jpg (184.61 KiB) Viewed 1308 times
And higher magnification ones.
2022-05-05-15.51.48 ZS Pmax retouched mod lab.jpg
2022-05-05-15.51.48 ZS Pmax retouched mod lab.jpg (151.96 KiB) Viewed 1308 times
2022-05-05-15.18.21 ZS Pmax retouched mod cropped lab.jpg
2022-05-05-15.18.21 ZS Pmax retouched mod cropped lab.jpg (239.63 KiB) Viewed 1308 times
I'm not too happy with the lighting or how I processed these, but I just find the structures really beautiful. Next time I think I will try the 2.5x photoeyepiece (this was the 3.3x one and it was what was on the microscope). Also, I cheated a bit with the 60x Splan Apo and used glycerine rather than immersion oil, as it is so much easier to clean off. Lighting was a using a mercury xenon lamp and I used a 405nm bandpass filter. Standard brightfield imaging with a basic Olympus Abbe condenser.

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Sjeiti
Posts: 18
Joined: Tue Feb 08, 2022 9:17 pm

Re: Couple of diatom slides using 405nm light

#2 Post by Sjeiti » Thu May 19, 2022 8:23 pm

Love that golden angle in the circular ones.

Greg Howald
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Joined: Tue Oct 20, 2020 6:44 am

Re: Couple of diatom slides using 405nm light

#3 Post by Greg Howald » Thu May 19, 2022 8:43 pm

Quite well done.

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woyjwjl
Posts: 325
Joined: Tue Jan 04, 2022 1:55 pm
Location: Wuhan, China

Re: Couple of diatom slides using 405nm light

#4 Post by woyjwjl » Fri May 20, 2022 6:16 am

JMC wrote:
Fri May 06, 2022 10:50 am
and used glycerine rather than immersion oil
I bought modern diatom slides, which can't withstand any wiping. :? So I've been worried about using immersion oil
Micrographers from China, thanks to the forum for providing a platform for exchange

JMC
Posts: 57
Joined: Wed Mar 30, 2022 9:21 am

Re: Couple of diatom slides using 405nm light

#5 Post by JMC » Fri May 20, 2022 8:20 am

Thanks all. I prefer buying older slides so I keep an eye out for them when I can. So far, they have been nice and robust. I am not a huge fan of immersion oil, and prefer to use glycerine where the objective allows (just because it is so much easier to clean afterwards).

Javier
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Joined: Tue May 09, 2017 11:19 am
Location: Buenos Aires, Argentina.

Re: Couple of diatom slides using 405nm light

#6 Post by Javier » Sun May 22, 2022 4:08 pm

Brilliant!

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