Plants under UV

[microplan]

Hello

My favorite activity with the microscope is fluorescence.
I like to observe different parts of plants in UV 365 nm.
Sometimes you can get interesting effects.
To make them stand out, I use stereo technology. I use ZereneStacker.
Pictures 1,2 are 375 files. And 5.6 is 465 files.
These are old photos from my home archive. I have quite a lot of them.
Some are of good quality and some are not.

Regards

[Hobbyst46]

Nice images that raise a question.

How comes there are so many hues in a plant autofluorescence image ? I could understand the red and yellow, and turquise, where do the violet shades come from ?
Of course in theory all visible spectrum is possible when you excite with UV, but still; and so many of the visible colors are intense...

Would it be possible to see a brightfield image of the same plant ?

[microplan]

Hobbysta46
Thank you very much for your time and the question that comes up.
I have no photos in visible light.
I take photos immediately in UV.
As I wrote, the piles have several hundred takes.
The photo shoot is therefore quite long and inconvenient.
I would have to return the lens to its original position and repeat the entire procedure under white light.
And there is no time for that.
Therefore, for now I will publish my old photos and if I find something interesting in the garden, I promise that I will take photos in UV and then in white light.

Regards

[LouiseScot]

Nice images that raise a question.

How comes there are so many hues in a plant autofluorescence image ? I could understand the red and yellow, and turquise, where do the violet shades come from ?
Of course in theory all visible spectrum is possible when you excite with UV, but still; and so many of the visible colors are intense...

Would it be possible to see a brightfield image of the same plant ?

The photos must be taken with a colour camera?

Louise

[PeteM]

Beautiful images. Thanks for skill and effort in making -- and then sharing them.

[microplan]

LouiseScot - I use Pentax digital cameras.
Pentax kx for taking pictures.
Pentax K III for filming.
I connect the cameras to the third light path using a self-made adapter.
And so I have to be careful to get the focus on the camera screen.

PeteM - thank you very much for the nice opinion.
But it seems to me that the appeal of the photo is, as always, a lucky accident.
It is true that a bit of effort is put into building the pile of photos, but the computer does all the work afterwards.
If the pile consists of several hundred shots, you have to wait all night for one final photo.
The attached photos are a stack of 370 and 380 shots.

Regards

[LouiseScot]

LouiseScot - I use Pentax digital cameras.
Pentax kx for taking pictures.
Pentax K III for filming.
I connect the cameras to the third light path using a self-made adapter.
And so I have to be careful to get the focus on the camera screen.

Regards

Yes, but the question was about the colour reproduction - so it is a colour camera. Are the colours produced just from fluorescence? You don't normally get so many different colours from autofluorescence, as far as I know? Perhaps there is a low level of white light coupled with long exposures?

Louise

[microplan]

Hello.

Hobbyst46 and Louisescot.

In response to your questions, I present photos of the cavernous space of my hard drive.
The photos are from 2019.
The same object illuminated with visible light and then UV light.
I just want to explain that I illuminate the object with three white light emitting diodes and then three UV emitting diodes.
Of course, I do not know if the white LEDs add other frequencies.
Besides, when I change the source of lighting, I do not always illuminate the same parts of the object to the same degree. Therefore, I do not know if this comparison can be of any value.
Before I post a photo on the forum, I adjust its resolution and size, as well as improve the exposure and sometimes sharpen it. I use GIMP for this.

Regards

[perrywespa]

Stunning images!

[Javier]

Gorgeous images!

[microplan]

Hello.
perrywespa and Javier.
Thank you very much for your nice opinion.
I'm glad you like my photos.
I personally like such warm colors in the photos.

Regards