Dileptus feeding

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tlansing
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Joined: Sat Aug 01, 2020 3:15 pm

Dileptus feeding

#1 Post by tlansing » Sat Jul 02, 2022 3:47 pm

I recently observed a number of the ciliated protist Dileptus actively feeding in a sample I had collected. I observed one of them ingesting a rotifer, basically stuffing it into its mouth! Unfortunately, I was not able to film this, but I did film one actively feeding. The photo below is a included to note a couple of features of Dileptus. Its most prominent feature is the contractile, highly mobile neck at the anterior of the cell. The mouth is positioned at the base of the neck. There are also a number of small contractile vacuoles along the dorsal surface of the cell. Some examples are shown at the arrowheads. There is also another larger contractile vacuole (CV) that can be seen. This photo was taken with a Canon 1300D camera and an Amscope adapter. The microscope is a Zeiss Photomicroscope III, 16x Neofluar objective, 1.25x optovar, DIC, flash.
Dileptus_photo.jpg
Dileptus_photo.jpg (37.12 KiB) Viewed 1095 times
Here is the video. Guest appearances are made by the ciliate Trachelius and a gastrotrich.
https://www.youtube.com/watch?v=NuraHqmVd44

Crusty
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Joined: Fri Mar 17, 2017 4:19 pm

Re: Dileptus feeding

#2 Post by Crusty » Sat Jul 02, 2022 11:31 pm

Really nice. Which objective did you use?

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Wes
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Re: Dileptus feeding

#3 Post by Wes » Sun Jul 03, 2022 11:18 am

Fascinating observation, Tim. Thanks for posting.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel

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Wes
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Re: Dileptus feeding

#4 Post by Wes » Sun Jul 03, 2022 11:19 am

Crusty wrote:
Sat Jul 02, 2022 11:31 pm
Really nice. Which objective did you use?
tlansing wrote:
Sat Jul 02, 2022 3:47 pm
The microscope is a Zeiss Photomicroscope III, 16x Neofluar objective, 1.25x optovar, DIC, flash.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel

tlansing
Posts: 336
Joined: Sat Aug 01, 2020 3:15 pm

Re: Dileptus feeding

#5 Post by tlansing » Sun Jul 03, 2022 12:16 pm

Hi Crusty and Wes, thanks for the kind comments. Crusty, as Wes noted, a 16x Neofluar objective was used and everything was the same as the photo except no flash.

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