Plankton sample

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imkap
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Plankton sample

#1 Post by imkap » Tue Sep 20, 2022 7:15 pm

A nice plankton sample my son took from the sea here. Don't know the exact species, except for the Creseis clava. If you know please tell us... September/October is a good time for catching plankton

Shot on a Olympus BH-2 BHS, with Wild Fluotar 3x and Olympus DPlan 10x objectives, apl, achr. condenser 1.4 with improvised darkfield, NFK 2.5x and Sony a6000.

The good thing about the APS-C crop sensor is that one can use a 3x objective on a 1.4 condenser without vignetting :mrgreen:

I'm not very talented with inventing video titles, maybe it should have a WOW! at the beginning. But no matter. :D


Sabatini
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Re: Plankton sample

#2 Post by Sabatini » Tue Sep 20, 2022 7:50 pm

Fantastic images.
Great work... thanks for sharing.

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xioz
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Re: Plankton sample

#3 Post by xioz » Wed Sep 21, 2022 1:05 am

Very nice images and video. Did you have to hunt for them on the slide(s)? Interested how abundant they are in open seawater.

Tom Jones
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Re: Plankton sample

#4 Post by Tom Jones » Wed Sep 21, 2022 3:33 am

Very nice! :D :D

Hobbyst46
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Re: Plankton sample

#5 Post by Hobbyst46 » Wed Sep 21, 2022 6:45 am

Beautiful !


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imkap
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Re: Plankton sample

#7 Post by imkap » Wed Sep 21, 2022 11:53 am

Thanks everyone, we're quite happy.
xioz wrote:
Wed Sep 21, 2022 1:05 am
Very nice images and video. Did you have to hunt for them on the slide(s)? Interested how abundant they are in open seawater.
He catches the individual zooplankton in a jar one by one. Has a good eye for spotting them, knows what they are before putting them on the microscope. I can't say I'm able to follow completely.

Javier
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Re: Plankton sample

#8 Post by Javier » Wed Sep 21, 2022 9:32 pm

Wow! I really enjoyed this one. Congratulations, fantastic dark field images and very interesting specimens!

Thanks for sharing.

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xioz
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Re: Plankton sample

#9 Post by xioz » Thu Sep 22, 2022 3:21 am

He catches the individual zooplankton in a jar one by one. Has a good eye for spotting them, knows what they are before putting them on the microscope. I can't say I'm able to follow completely.
Man, that is actually possible? :o I can imagine the steps and patience needed, but it is a lot of work!
I just stick pipette in somewhere likely, use one drop on the slide & hope, and then spend 20 mins trying to find something. ;)

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xioz
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Re: Plankton sample

#10 Post by xioz » Thu Sep 22, 2022 3:21 am

He catches the individual zooplankton in a jar one by one. Has a good eye for spotting them, knows what they are before putting them on the microscope. I can't say I'm able to follow completely.
Man, that is actually possible? :o I can imagine the steps and patience needed, but it is a lot of work!
I just stick pipette in somewhere likely, use one drop on the slide & hope, and then spend 20 mins trying to find something. ;)

mrlowndes
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Re: Plankton sample

#11 Post by mrlowndes » Tue Oct 04, 2022 10:02 pm

Brilliant!

May I ask what sort of slides/ cover slips you use to allow the specimens to move around so freely? Also, could you give more details on your improvised darkfield? As much information as possible would be great. I am trying to find out how to achieve the best darkfield I can.

Fantastic images!

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imkap
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Re: Plankton sample

#12 Post by imkap » Wed Oct 05, 2022 6:49 am

mrlowndes wrote:
Tue Oct 04, 2022 10:02 pm
Brilliant!

May I ask what sort of slides/ cover slips you use to allow the specimens to move around so freely? Also, could you give more details on your improvised darkfield? As much information as possible would be great. I am trying to find out how to achieve the best darkfield I can.

Fantastic images!
Hi, thanks

No coverslip, here is the info on DF:
viewtopic.php?f=6&t=16113&p=126726#p126726

macnmotion
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Re: Plankton sample

#13 Post by macnmotion » Wed Oct 05, 2022 3:22 pm

Beautiful imagery. Thanks for sharing this.

mrlowndes
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Re: Plankton sample

#14 Post by mrlowndes » Wed Oct 05, 2022 7:14 pm

imkap wrote:
Wed Oct 05, 2022 6:49 am

Hi, thanks

No coverslip, here is the info on DF:
viewtopic.php?f=6&t=16113&p=126726#p126726
Thanks for your reply. Did you use well slides or normal slides?

Great that you didn't get any detritus behind the specimens that would have reduced your contrast. I"m having a terrible time trying to clean the slides well enough for DF imaging

Ncs
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Re: Plankton sample

#15 Post by Ncs » Wed Oct 05, 2022 7:40 pm

Wow this is amazingly clear and vivid footage. Very nice! I'm not familiar with the jargon surrounding the technical aspects of capturing this footage but am nonetheless interested. Not shooting with a vignette is very nice. I have to bump my camera into crop frame mode to achieve this which is unfortunate. Definitely a win for APS-C!

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imkap
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Re: Plankton sample

#16 Post by imkap » Thu Oct 06, 2022 5:34 am

mrlowndes wrote:
Wed Oct 05, 2022 7:14 pm

Thanks for your reply. Did you use well slides or normal slides?

Great that you didn't get any detritus behind the specimens that would have reduced your contrast. I"m having a terrible time trying to clean the slides well enough for DF imaging
I used a normal slide, cleaned with soap and water and after with my t-shirt and breath :)
I did some video editing on the computer to make the background darker. Darkfield is pretty unforgiving, slides and condenser must be clean...
Ncs wrote:
Wed Oct 05, 2022 7:40 pm
Definitely a win for APS-C!
Yes, the main downside of it became an upside.
It is great that with lower mag objectives you have a deeper depth of field, so for lower magnification stuff, which doesn't fill the objective image circle aps-c actually might be better :D

Although you can use crop mode on a FF, but then it is not a FF anymore :D

macnmotion
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Re: Plankton sample

#17 Post by macnmotion » Thu Oct 06, 2022 7:55 am

imkap wrote:
Thu Oct 06, 2022 5:34 am
Although you can use crop mode on a FF, but then it is not a FF anymore :D
I can't believe I didn't know my Nikon FF had crop modes. For video it has a 1.5 crop option (it has 1.2 and 1.5 for photo), and still fills 1920x1080. I'm seeing things I've never been able to see before. So cool.

Ncs
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Re: Plankton sample

#18 Post by Ncs » Fri Oct 07, 2022 1:10 am

imkap wrote:
Thu Oct 06, 2022 5:34 am


Yes, the main downside of it became an upside.
It is great that with lower mag objectives you have a deeper depth of field, so for lower magnification stuff, which doesn't fill the objective image circle aps-c actually might be better :D

Although you can use crop mode on a FF, but then it is not a FF anymore :D
I actually do like APS-C quite a lot. The only real downside in my experience is typically their noise patterns are more present and in my opinion more displeasing but keeping a low iso is a workaround for that (and a really good excuse to learn lighting, a skill that will make any potato produce good images) The 1/3 stop of light lost over full frame was also a drawback however the smaller field I think is much to the advantage of the aps-c camera. Wide angle photography is still very achievable on an aps-c system but they outrun FF in long lens photography. An incredibly versatile sensor size. And as someone coming from the professional video space, a deeper depth of field is a bonus, I hardly shoot anything stopped below f4. Don't even get me started on being on set trying to pull focus on a subject when the director of photography says they want to shoot at f1.8.....

SolomonChmielewski
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Re: Plankton sample

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