My mud puddle culture has matured and articulated after a month or so. Protists have gathered in large numbers to feed on them. Pockets of early-stage biofilm have formed on the surface of the water. Most of the bacteria are still moving independently, have not merged into straight biofilm. I do find packets of mature biofilm floating in the culture, however.
https://youtu.be/xvSPV--A614
Protists Invading Bacteria Colony
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- Location: Winnipeg, Manitoba, Canada