MicrobeHunter.com Microscopy Forum

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Euplotes dividing.
Strawbridge Lake, mini pond water sample
Plan 10X objective
Nikon S Cb microscope

I'm not getting anything with this link. Is there a reason you're using Onedrive instead of something like Youtube?

macnmotion,

Works fine for me.

There are several reasons why not at Youtube.
I have two Youtube channels- one for personal and history stuff and misc and the other for guitar playing.
I really do not want to have 3 Youtube channels.

On OneDrive if someone likes the video or photo or whatever they can download it if they want to FULL RESOLUTION size.
Plus for my own use I am backing up in the Cloud all my microscopy stuff in one folder.

I guess there are some pitfalls to my chosen way but that is what I want to do.
Yes there are other options. I was just doing what I thought I liked.

Works fine for me

Dennis, I am just a bit curious; what is the width of the view when you use your camera with your 10x objective?
.. these seem much larger than the ones that I get to see.

Chas,
I use a AmScope MD500 USB camera in one of my eye pieces (5MP)

In the other eye piece I see more than the camera picks up but of course it needs to turn it into a rectangle.
Then what you see on the computer monitor in the software is what the picture or video will be.

I use the biggest setting (as shown) I learned not to change settings mid usage as then rock and rolls it and I had to reinstall software.
Now I am gentle and even choose File/Exit vs closing the window at the corner.
I thought at the lowest setting the Rotifer twirlers were seen better but someone said it looked pixelated so I went back to the largest resolution size.

Very clear images.

Dennis, I was meaning more; If you were to stick a ruler under your 10x objective what sort of proportion of the gap between the mm lines would you get to see /photograph?
Hard to estimate I am sure.
[easy on my CTS as I get to see both of the mm markings ]

Chas,
No idea what you just said.

Every microscope is different and lenses different so height from bottom of objective and top of cover slip is different
All that does not matter in what the AmScope camera picks up.

Oh you mean horizontally.
I took a cover slip and at every 1/8 inch drew a line with a blue pen.
I was amazed to see through the eye piece my field of view is tops 1/4 of an 1/8 of an inch. Probably like 1/64 of an inch ??
Through the AmScope too blurry. but seems to be about same.

I do not know about Euplotes much but I know say Paramecium-
Babys could need a bigger objective to see then as they keep growing you need a smaller objective size.

Chas,
I made this having a 1/16 of an inch space between the two red marker lines.



Using the Nikon microscope with Plan 10X objective I could see a smidge of each red line through the eye piece but no red on AmScope software.
So then I made another space using red lines having 1/32 inch spacing between. I could, of course, see the space and more of the red lines in eye piece view
but still no red lines in AmScope USB camera software screen capture,

So the answer would be on that microscope and that plan 10X objective I see a little over 1/16" of an inch
and with the AmScope USB camera it cuts it down to less being less than 1/32 of an inch.

Actually I see how high the 10X is up off the stage. You would have to focus in on a water sample, then pull that slide off and stick my measuring slide on at that point.
Just did- Same results.

Thanks Dennis, I have just had a go with a grey chinese horseshoe scope and a steel rule and I get to see 1/16inch with the 10x objective and a 10x eyepiece.
But even if your the amscope camera acts like a 20x eyepiece my Euplotes if thats what they are, are very small (babies, as you say).
Much smaller too than the Euplotes in Martin Parnham's video in his 'feeding vortices' post.

-I have just tried his diluted milk trick on some vorticella and it is really fun!

Chas,

I don't think the AmScope magnifies things more than the 160mm

It shows less because it cuts a rectangle image in the middle of the circle.

The eye piece side has the 10X with focus.

I could be incorrect.

Regardless though the size is what I said. AmScope width of capture at 10X is less than 1/32 of an inch.