I found some nice Rotifers of the genus Asplanchna a few months ago. Since they are quite big, they made a great opportunity to try my DIY polarized system, which is very limited due to the lack of power of the lamp of my current scope.
The sequence of the video is the following:
1- Oblique illumination
2- Dark field + polarizing filter 1 (which cast a bluish hue)
3- Polarizing filter 2 (which cast a grayer hue)
4- Oblique illumination + polarizing filter 1
5- Dark field
The entire sequence was made at 100x with an iPhone 5s mounted on an Amscope b120. I would love to replace the original 1W with a 3W LED in the future to get better at polarized light, which I found fascinating.
Thanks for watching!
Javier
Asplanchna or Tricks of the light
Asplanchna or Tricks of the light
Last edited by Javier on Thu Jun 01, 2023 6:25 pm, edited 1 time in total.
- ImperatorRex
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Re: Asplanchna or Tricks of the light
Hi Javier,
a nice video, well done. The Rotifier obviously makes a good object to demonstrate the different effects.
Just the simple bright field illumination is missing
a nice video, well done. The Rotifier obviously makes a good object to demonstrate the different effects.
Just the simple bright field illumination is missing
Re: Asplanchna or Tricks of the light
Thank you, Jochen.ImperatorRex wrote: ↑Wed May 31, 2023 4:36 pmHi Javier,
a nice video, well done. The Rotifier obviously makes a good object to demonstrate the different effects.
Just the simple bright field illumination is missing
You are right, I just forgot about bright-field when taking the videos.
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Re: Asplanchna or Tricks of the light
A polarizer does a good job of eliminating a lot of glare and improving small linear details like cell walls.
I'm currently learning to better use my oblique stops. Do you have a favorite oblique lighting shape? Or shape for specific objective?
I'm currently learning to better use my oblique stops. Do you have a favorite oblique lighting shape? Or shape for specific objective?
Re: Asplanchna or Tricks of the light
Honestly, after trying a few shapes, I opted for using the filter holder with my home-made dark filter in it half open (depending on the objective) to get oblique illumination. To my eye, subtle differences in shape didn't make a difference. Having the option to regulate the position of the filter holder seems more versatile.Sure Squintsalot wrote: ↑Wed May 31, 2023 7:13 pmA polarizer does a good job of eliminating a lot of glare and improving small linear details like cell walls.
I'm currently learning to better use my oblique stops. Do you have a favorite oblique lighting shape? Or shape for specific objective?