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Thanks for the details. So you shoot high speed bursts while rotating the focuser smoothly, at least that's what it sounds like. Unfortunately my D750 doesn't shoot quickly enough for that, so I'm limited to stacks from 1080P video for moving things.hkv wrote: ↑Fri Jan 12, 2024 7:45 amThank you all for the kind feedback! The stack was shot using a Sony A9 so the images were shot in a rapid series, but not video. The image is sort of hand stacked or stacked in batches. I decided what features I needed to include in the image (food, edge, surface texture, nuclei, food, pseudopods, etc). Then I stacked the frames containing these features individually in Zerene. After having all sub stacks done (10 ish), I then ran Zerene again and made the final composition out of the sub stacks I did in the previous steps. Then some manual retouching in Zerene to take out artefacts or avoid cases where I had multiple features in focus and a selection was needed what to prioritise. The background is a from a stack that I shot that deliberately is way out of focus so it gets a clean look.
I did not use the rapid burst mode in this case. The amoeba moved slowly enough to just use single shots and refocus. The only time I have used burst mode and focusing during burst was when photographing Vorticella.macnmotion wrote: ↑Fri Jan 12, 2024 10:40 amThanks for the details. So you shoot high speed bursts while rotating the focuser smoothly, at least that's what it sounds like. Unfortunately my D750 doesn't shoot quickly enough for that, so I'm limited to stacks from 1080P video for moving things.hkv wrote: ↑Fri Jan 12, 2024 7:45 amThank you all for the kind feedback! The stack was shot using a Sony A9 so the images were shot in a rapid series, but not video. The image is sort of hand stacked or stacked in batches. I decided what features I needed to include in the image (food, edge, surface texture, nuclei, food, pseudopods, etc). Then I stacked the frames containing these features individually in Zerene. After having all sub stacks done (10 ish), I then ran Zerene again and made the final composition out of the sub stacks I did in the previous steps. Then some manual retouching in Zerene to take out artefacts or avoid cases where I had multiple features in focus and a selection was needed what to prioritise. The background is a from a stack that I shot that deliberately is way out of focus so it gets a clean look.
Looks very similar in shape, so most likely you are correct.WWWW wrote: ↑Sat Jan 13, 2024 10:16 amThe rectangular structures are possibly the cells of the desmid: Bambusina borreri (= Bambusina brebissonii)
See: https://www.desmids.nl/maand/nederlands ... sonii.html
And of course: https://youtu.be/QxyQN2KNWb0