Negative and Positive Bias via Post Processing rotation in a Pleurosigma sp.

[75RR]

Plan 40x/0.65, DIC, 130µm, 9 image stack in Photoshop

Image was taken in Positive Bias diagonally in Landscape mode.
Curiously when the image was rotated clockwise to Portrait format - it appears to be Negative Bias,
when rotated anticlockwise it maintains its Positive Bias.

[hkv]

Very nice! What do you mean by positive and negative bias?

[75RR]

Thanks hkv

What do you mean by positive and negative bias?

Scroll down to Fig. 2

https://www.microscopyu.com/tutorials/d ... -alignment

[kit1980]

Nice image!
At first, I saw negative bias on the left image, but after scrolling down and up now I see only positive bias on all images.

[75RR]

Thanks kit1980,

At first, I saw negative bias on the left image, but after scrolling down and up now I see only positive bias on all images.

Happens to me on occasion, as it did here when I added the second positive image.
I believe that is the eye, or better said the mind rectifying an "anomaly".

Will remove the landscape positive image, see if that helps.

[Crater Eddie]

Pretty cool. I get the same effect when I look at these... for several seconds they look like positive and negative, then suddenly they are both positive.
CE

[btschumy]

It's a biological Necker cube!

[KurtM]

It even works as a cross eye stereo pair! Not the most amazing 3D effect I've ever seen, but still fairly amusing to mind as idle as mine.