Tapeworm! This time attempting oblique lighting

[Pat Thielen]

Hello everyone! Through the forums I came across a technique that people refer to as oblique lighting. So, I asked about it and thought I'd give it a whirl. So, this is my first attempt. I thought the tapeworm slide from Triarch Inc. would be a good specimen as I wanted to work with something a bit larger to see what effect I could achieve. This is a 12 image stack of a dog tapeworm. As always, let me know what you think and how I might improve my technique.

The technical details are actually rather simple. I was using a 10x plan phase-contrast objective with the P1 position on the phase-contrast turret in place. I then slowly turned it towards the P2 position and found a spot that seemed to give a slightly 3D effect. One major problem I came across was the right side of the image was underexposed by (I'm guessing) one stop. There is a very small area where you can turn the phase-contrast turret to give this effect without a great deal of light flare. Perhaps some of you have encountered this as well and may have some advice to help me improve.

Thanks much!

[Crater Eddie]

Nice oblique effect! About the uneven exposure effect, I get that too when using my LOMO oblique condenser.
CE

[Pat Thielen]

Nice oblique effect! About the uneven exposure effect, I get that too when using my LOMO oblique condenser.
CE

Do you have a work-around for the uneven exposure? I did some very heavy editing to this image to fix it but the uneven exposure was actually quite pronounced. Hopefully I'll get this all figured out!

[75RR]

I also use my Phase condenser to achieve oblique, I combine rotating the brightfield port with the condenser diaphragm setting.
Does not take much movement to get a good effect.

[Pat Thielen]

I also use my Phase condenser to achieve oblique, I combine rotating the brightfield port with the condenser diaphragm setting.

I'll give that a try next time. Thanks! Do you have trouble with uneven exposures?

[75RR]

Do you have trouble with uneven exposures?

There is always some - comes with the territory.

[Pat Thielen]

Are there subjects better suited for oblique lighting? Just curious.