Marine Copepods

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
hkv
Posts: 972
Joined: Sun Nov 02, 2014 8:57 pm
Location: Sweden
Contact:

Marine Copepods

#1 Post by hkv » Tue Apr 04, 2017 7:44 pm

Following up on my previous post on freshwater copepods:

Link:
viewtopic.php?f=6&t=4775

Here are a few marine copepods. They are much smaller, around half the size, but also very beautiful when closing in.
No DIC this time, but a comparison between Pol, Darkfield and Fluorescence of the same subject.

DarkField 4X objective:

Image

Polarised light with retarder, 4X objective:

Image

Autofluorescence, 4X objective:

Image

Egg sack. Autofluorescence, 10X objective:

Image

One interesting thing with EPI-Fluo is that the higher power objective you use, the brighter the images gets. This is completely the opposite towards regular techniques. All light from the lamp is passing the objective, so the smaller the aperture, the more concentrated light. This is very convenient as it enables you to get excellent illumination also at higher magnifications.

This is the head in autofluorescence at 10X:

Image

As always, you will find images with four times the resolution on my flickr page. Link in my signature footer.
Last edited by hkv on Wed Apr 05, 2017 4:24 pm, edited 1 time in total.

JimT
Posts: 3247
Joined: Fri Oct 24, 2014 1:57 pm

Re: Marine Copepods

#2 Post by JimT » Tue Apr 04, 2017 8:30 pm

Really like the autofluorescence images and the detail they reveal. Well done.

User avatar
billben74
Posts: 1020
Joined: Sat Dec 26, 2015 10:33 pm
Location: Cambridge, UK

Re: Marine Copepods

#3 Post by billben74 » Tue Apr 04, 2017 8:45 pm

Really nice both in terms of revealing detail and being very beautiful.
Nice to be able to compare as well. Love the glowing eye on the polarised using the retarder
A treat.

User avatar
zzffnn
Posts: 2738
Joined: Sat Jun 20, 2015 3:57 am
Location: Houston, Texas
Contact:

Re: Marine Copepods

#4 Post by zzffnn » Wed Apr 05, 2017 12:41 am

Very beautiful!

charlie g
Posts: 1485
Joined: Mon Oct 20, 2014 7:54 pm

Re: Marine Copepods

#5 Post by charlie g » Wed Apr 05, 2017 1:22 pm

Thank you for the beauty of this variety of illumination methods with this copepod,hkv. The 'autoflourescenance' (?sp?) image of the egg sack is perhaps more than the 4X objective depicting this organism in the other images?

Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?

That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?

Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.

charlie guevara

User avatar
Crater Eddie
Posts: 1835
Joined: Wed Nov 12, 2014 4:39 pm
Location: Illinois USA

Re: Marine Copepods

#6 Post by Crater Eddie » Wed Apr 05, 2017 1:24 pm

Very beautiful images, and showing great detail. Extremely interesting.
CE
Olympus BH-2 / BHTU with Canon T3i camera mounted
LOMO BIOLAM L-2-2 with Olympus E-P1 MFT camera mounted
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)

User avatar
hkv
Posts: 972
Joined: Sun Nov 02, 2014 8:57 pm
Location: Sweden
Contact:

Re: Marine Copepods

#7 Post by hkv » Wed Apr 05, 2017 4:29 pm

charlie g wrote:Thank you for the beauty of this variety of illumination methods with this copepod,hkv. The 'autoflourescenance' (?sp?) image of the egg sack is perhaps more than the 4X objective depicting this organism in the other images?

Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?

That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?

Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.

charlie guevara
Thanks all for looking! Yes, it should have said 10X! I had it right in the flickr page, but wrong here. It is now adjusted.

Yes, it was a different subject. Not all had egg sacks.

Agree on the eye spots. Fascinating. The POL color came out really bright.

They were alive and kicking and I had trouble getting them to sit still. The one with 3 different illuminations was squeezed tight between the cover glass and the slide so he was ore or less still during the photo session. He is now dead. :?

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Marine Copepods

#8 Post by gekko » Wed Apr 05, 2017 11:06 pm

In my view, all are exceptionally well done images: very beautiful aesthetically, but sharp, detailed, with excellent contrast and color.

User avatar
hkv
Posts: 972
Joined: Sun Nov 02, 2014 8:57 pm
Location: Sweden
Contact:

Re: Marine Copepods

#9 Post by hkv » Wed Apr 05, 2017 11:08 pm

Thanks Gekko!

Post Reply