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Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 9:13 am
by 75RR
Planapo 63x/1.4, DIC, 250µm length, stacked and stitched in Photoshop, Marine sample, Alboran Sea.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 10:21 am
by MichaelG.
Fabulous image !
At this resolution I can’t see any evidence of the stitching
... is it really that good ?
MichaelG.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 10:51 am
by mrsonchus
MichaelG. wrote: ↑Sun Feb 09, 2020 10:21 am
Fabulous image !
At this resolution I can’t see any evidence of the stitching
... is it really that good ?
MichaelG.
Ditto Michael!
It really is -
when combined with 75's consummate skill with this work - fantastic inspiration and goal for others.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 1:00 pm
by Hobbyst46
Great image (as usual) !!
The diatoms seem to feed well on the western side of the Mediterranean, and grow to such size.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 2:36 pm
by WhyMe
Very nice!
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 3:59 pm
by 75RR
Many thanks MichaelG, mrsonchus, Hobbyst46 and WhyMe
At this resolution I can’t see any evidence of the stitching
Photoshop stitching (photomerge) is quite seamless and easy to use. Choose images - press button.
If there are problems it is usually either a lack of sufficient image overlap or non matching overlaps.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 6:18 pm
by Wes
Beautiful work 75'
How do you do the stitching? I tried to load the files to be stacked via "File>Script>Load Files Into Stack" and then "Edit>Auto-Align Layers".
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 6:45 pm
by 75RR
Thanks Wes
I normally use Adobe Bridge, I find it easier.
If you are going to go direct then there are 3 steps:
"File>Script>Load Files Into Stack"
"Edit>Auto-Align Layers"
"Edit>Auto-Blend Layers> Panorama"
Note: You need to highlight all files that are to be processed.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 7:52 pm
by mrsonchus
Hi 75',
I've just started using Bridge/Photoshop (and Lightroom-Classic for fast batch edits) and have found the stacking to be pretty good in PS also. Is there a way to know what type of stacking PS is performing, or even maybe to alter parameters do you know? Perhaps the absence of parameters points to 'Pyramid' stacking?
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 8:58 pm
by 75RR
Hi mrsonchus, can't say I have found anyway to fine tune Photoshop stacking/stitching (seems to me to be mostly automated) nor do I know how it works. Shame on me!
I started using Photoshop for this as I already had it. I find it useful, but suspect it is a little basic as it is just another addition to what is a massive photo editing software program.
I believe dedicated programs are necessarily better, but I always seem to find another use for the money needed for one.
Re: Nitzschia sigmoidea
Posted: Sun Feb 09, 2020 9:09 pm
by mrsonchus
I think you're right 75' - I definitely see different effects with the various (Weighted-Average, Depth-Map, Pyramid) options in Helicon Focus - especially I suspect with the very often 'soft' images of obscure detail within a mounted-section rather than optimally at the coverslip's underside.