Rheinberg filters and other Techniques

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RobBerdan
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Rheinberg filters and other Techniques

#1 Post by RobBerdan » Mon Jun 01, 2020 3:35 am

Rheinberg filters have been around a long time. You can make them your self or purchase a set on E-bay for about $30. These simple filters can add colour contrast with almost any microscope.
I am attaching some photos using a variety of techniques, polarization, bright field, dark-field, DIC, Rheinberg, Phase contrast for comparison. For those that might not be familiar with these simple filters I wrote an article on my web site 2 years ago: https://www.canadiannaturephotographer. ... s2017.html

I think for their cost Rheinberg filters are good value. I made some, but they were not satisfactory, the plexi-glass filters availalble on Ebay were better and well worth their cost. In my article I link to a book by Mike Shaw that shows you how to make these filters out of thin acetate for those that want to do it yourself. There are also links to how others who make them with a colour laser printers. Most of the articles talk about designing them differentt sizes for different objectives which I don't think are necessary. They work well with 4, 10, 20 and 40X objectives. I put the filters on a cardboard box with a hole in it under the condensor or use a filter holder holder that I can raise and lower under the condenser with different objectives (I just use my hand and it works fine). Sometimes you get a better colour effects by holding the filter slightly to the side of the light path.

Polarization filters can also be added to any microscope easily - the Microbe hunter has a YouTube video and there are lots of tutorials online that show how to do it.

You can download and read the original paper from 1896 by Julius Rheinberg if you like here: https://www.canadiannaturephotographer. ... on1896.pdf

Cheers'
RB
Attachments
Rotifer (Brachionus) with polarization microscopy
Rotifer (Brachionus) with polarization microscopy
DSC_0597.jpg (75.08 KiB) Viewed 3035 times
Volvoz - Brightfield, Darkfield, Phase contrast, DIC, Rheinberg and Epi-fluorescence
Volvoz - Brightfield, Darkfield, Phase contrast, DIC, Rheinberg and Epi-fluorescence
6techniques.jpg (158.46 KiB) Viewed 3035 times
Rotifer (Brachionus) with Rheinberg
Rotifer (Brachionus) with Rheinberg
DSC_0566.jpg (93.34 KiB) Viewed 3035 times
Algae with Rheinberg filter
Algae with Rheinberg filter
DSC_0185.jpg (169.33 KiB) Viewed 3035 times
Dancing Daphnia photographed with different Rheinberg filters
Dancing Daphnia photographed with different Rheinberg filters
Rheingberg-1.jpg (61.43 KiB) Viewed 3035 times

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75RR
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Re: Rheinberg filters and other Techniques

#2 Post by 75RR » Mon Jun 01, 2020 5:55 am

Always nice to see illumination technique comparisons of the same subject.

Image
Volvoz - Brightfield, Darkfield, Phase contrast, DIC, Rheinberg and Epi-fluorescence

Of the more accessible ones, I find Oblique, Darkfield and Phase the most useful.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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KD Arvidsson
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Re: Rheinberg filters and other Techniques

#3 Post by KD Arvidsson » Wed Jun 17, 2020 8:06 pm

Beautifully and very interesting!! //KD
Microscope Nikon Labophot 2
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw

DonSchaeffer
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Re: Rheinberg filters and other Techniques

#4 Post by DonSchaeffer » Wed Jun 17, 2020 9:14 pm

These are amazing!

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Maryfox
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Re: Rheinberg filters and other Techniques

#5 Post by Maryfox » Thu Jun 18, 2020 1:19 pm

I am inspired to try these.

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