microspectrophotometry

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stevelev1
Posts: 3
Joined: Mon Apr 19, 2021 1:01 am

microspectrophotometry

#1 Post by stevelev1 » Mon Apr 19, 2021 1:12 am

Does anyone know of a way (hopefully not too expensive) that I can attach an infrared spectrometer (including a slit and diffraction grating) to the vertical tube of a trinocular microscope along with a digital camera and associated software that can produce the infrared spectra of the specimen being viewed through the binocular eyepiece? This allows identification and study of the elements and molecular components (infrared vibrational spectra) of a specific portion of the specimen. The field of microspectrophotometry is facinating but the mainstream instruments are prohibitively expensive for the individual hobbyist microscopist.

Gasman
Posts: 153
Joined: Sat Jan 09, 2016 1:36 pm
Location: North East UK

Re: microspectrophotometry

#2 Post by Gasman » Mon Apr 26, 2021 9:44 pm

Steve
I’ve been adapting my Astro spectro gear for use with my Leica trinocular scope.Its an Alpy 600 used for the visual part of the spectrum but not as far as UV. This is a mono Atik 314l cooled ccd but could easily be replaced with a DSLR!.
248057FE-460F-4530-80C8-8D885E707F5D.jpeg
248057FE-460F-4530-80C8-8D885E707F5D.jpeg (308.33 KiB) Viewed 1337 times
Taking an image from a neon/Argon light source gives this
819C586E-B5B6-4198-8817-AB20670BEE9D.jpeg
819C586E-B5B6-4198-8817-AB20670BEE9D.jpeg (126.49 KiB) Viewed 1337 times
and putting it into Spectragryph software (This was all experimental and as you can see NOT a calibrated plot)
CE1BA404-264F-4D2D-8844-E59D088FF6A3.jpeg
CE1BA404-264F-4D2D-8844-E59D088FF6A3.jpeg (175.1 KiB) Viewed 1337 times
So can’t really see a problem placing a UV spectroscope in a similar position.

Steve
Last edited by Gasman on Mon Apr 26, 2021 10:05 pm, edited 2 times in total.

Gasman
Posts: 153
Joined: Sat Jan 09, 2016 1:36 pm
Location: North East UK

Re: microspectrophotometry

#3 Post by Gasman » Mon Apr 26, 2021 9:55 pm

You might be wondering what the little guide ccd at the side is doing?.
It’s to show where to place the 23micron slit on the slide for entry to the spectroscope. The same use with a telescope placing a star on the slit!.
884801B9-231D-41D5-B9E1-62ACED03751A.jpeg
884801B9-231D-41D5-B9E1-62ACED03751A.jpeg (223 KiB) Viewed 1334 times
Steve

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