A DIY Raspberry Pi Based Computational Microscope

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LouiseScot
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A DIY Raspberry Pi Based Computational Microscope

#1 Post by LouiseScot » Fri Apr 23, 2021 4:31 pm

I came across this idea in Nature:

https://www.nature.com/articles/s41598-019-43845-9.pdf

If I had more time I might have a go at it myself but too many other projects at the moment! Still, it's quite interesting and maybe has some scope as a basis for brainwaving some other ideas, if you know what I mean :)

Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo

LouiseScot
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Re: A DIY Raspberry Pi Based Computational Microscope

#2 Post by LouiseScot » Fri Apr 23, 2021 4:52 pm

This takes the idea to a higher level and adds fluorescence microscopy! A 100 Euro Lab!

https://journals.plos.org/plosbiology/a ... io.2002702

Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo

dtsh
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Re: A DIY Raspberry Pi Based Computational Microscope

#3 Post by dtsh » Fri Apr 23, 2021 5:15 pm

There's OpenFlexure as well. I haven't done anything but look at the project.
https://openflexure.org/

LouiseScot
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Re: A DIY Raspberry Pi Based Computational Microscope

#4 Post by LouiseScot » Fri Apr 23, 2021 5:28 pm

dtsh wrote:
Fri Apr 23, 2021 5:15 pm
There's OpenFlexure as well. I haven't done anything but look at the project.
https://openflexure.org/
Yeah, similar idea though maybe a bit crude. The two I posted were cited in the Sanderson prism DIC paper from the diy DIC thread :)
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo

dtsh
Posts: 977
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Location: Wisconsin

Re: A DIY Raspberry Pi Based Computational Microscope

#5 Post by dtsh » Fri Apr 23, 2021 6:11 pm

LouiseScot wrote:
Fri Apr 23, 2021 5:28 pm
dtsh wrote:
Fri Apr 23, 2021 5:15 pm
There's OpenFlexure as well. I haven't done anything but look at the project.
https://openflexure.org/
Yeah, similar idea though maybe a bit crude. The two I posted were cited in the Sanderson prism DIC paper from the diy DIC thread :)
Louise
Certainly not promoting it as better, just another thing that someone might find interesting or useful that happens to be an rpi powered microscope.
I see the DIY DIC thread, but I recognize that's out of my league for now. :P

LouiseScot
Posts: 1167
Joined: Tue Jul 07, 2020 1:51 pm
Location: Scotland

Re: A DIY Raspberry Pi Based Computational Microscope

#6 Post by LouiseScot » Fri Apr 23, 2021 7:21 pm

dtsh wrote:
Fri Apr 23, 2021 6:11 pm
LouiseScot wrote:
Fri Apr 23, 2021 5:28 pm
dtsh wrote:
Fri Apr 23, 2021 5:15 pm
There's OpenFlexure as well. I haven't done anything but look at the project.
https://openflexure.org/
Yeah, similar idea though maybe a bit crude. The two I posted were cited in the Sanderson prism DIC paper from the diy DIC thread :)
Louise

I see the DIY DIC thread, but I recognize that's out of my league for now. :P
It's only a couple of pieces of polycarbonate, a couple of lenses, and some polarisers :P
I'm having a go :)

Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo

Hobbyst46
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Re: A DIY Raspberry Pi Based Computational Microscope

#7 Post by Hobbyst46 » Fri Apr 23, 2021 9:28 pm

LouiseScot wrote:
Fri Apr 23, 2021 7:21 pm
It's only a couple of pieces of polycarbonate, a couple of lenses, and some polarisers :P
I'm having a go :)
I'm crossing my fingers for your success. Hopefully it will work even for my finite TL 70-years old microscope...

LouiseScot
Posts: 1167
Joined: Tue Jul 07, 2020 1:51 pm
Location: Scotland

Re: A DIY Raspberry Pi Based Computational Microscope

#8 Post by LouiseScot » Fri Apr 23, 2021 9:52 pm

Hobbyst46 wrote:
Fri Apr 23, 2021 9:28 pm
LouiseScot wrote:
Fri Apr 23, 2021 7:21 pm
It's only a couple of pieces of polycarbonate, a couple of lenses, and some polarisers :P
I'm having a go :)
I'm crossing my fingers for your success. Hopefully it will work even for my finite TL 70-years old microscope...
Thanks! It really needs an infinite system in order to insert the extra optics above the objective though maybe anything's possible...
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo

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