How To Calibrate Camera Software

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FatBassPlayer
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How To Calibrate Camera Software

#1 Post by FatBassPlayer » Fri May 13, 2022 10:39 am

I'm using a little Bressers MikrOptic eyepiece camera (pull the eyepiece out, pop the camera in) and getting some nice results. However, the software has the ability to insert a scale bar on top of an image or video.

I cannot for the life of me figure out how to calibrate that, and not having any joy searching online.

Has anyone done this, and are there easy steps to follow?

TIA :)
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blekenbleu
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Re: How To Calibrate Camera Software

#2 Post by blekenbleu » Fri May 13, 2022 4:52 pm

Microscope calibration slides are available on eBay for around US$10.
Metaphot, Optiphot 1, 66; AO 10, 120, EPIStar, Cycloptic

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FatBassPlayer
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Re: How To Calibrate Camera Software

#3 Post by FatBassPlayer » Fri May 13, 2022 10:47 pm

blekenbleu wrote:
Fri May 13, 2022 4:52 pm
Microscope calibration slides are available on eBay for around US$10.
I've got one, and have photographed various bits of it at various magnifications (40x, 100x, 400x) but have no idea what to do next.
Picasso is dead,
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Einstein is dead,
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Alexander
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Re: How To Calibrate Camera Software

#4 Post by Alexander » Sat May 14, 2022 4:38 am

FatBassPlayer wrote:
Fri May 13, 2022 10:47 pm

I've got one, and have photographed various bits of it at various magnifications (40x, 100x, 400x) but have no idea what to do next.
Read the instruction for the software.

Basically the process is to put the slide in focus and klick something like "calibrate", then mark a known distance (the scale on the slide) in the software and store the result together with the information which objective was used and the marked distance. Repeat for every objective you have. After that you may use the function named "measure" or similar using the proper one of the stored profiles. You mark a distance on your specimen and the software gives you the distance measured.

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FatBassPlayer
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Re: How To Calibrate Camera Software

#5 Post by FatBassPlayer » Sat May 14, 2022 11:01 am

Alexander wrote:
Sat May 14, 2022 4:38 am
FatBassPlayer wrote:
Fri May 13, 2022 10:47 pm

I've got one, and have photographed various bits of it at various magnifications (40x, 100x, 400x) but have no idea what to do next.
Read the instruction for the software.

Basically the process is to put the slide in focus and klick something like "calibrate", then mark a known distance (the scale on the slide) in the software and store the result together with the information which objective was used and the marked distance. Repeat for every objective you have. After that you may use the function named "measure" or similar using the proper one of the stored profiles. You mark a distance on your specimen and the software gives you the distance measured.
I think I've got it! Thank you for this!
Picasso is dead,
Mozart is dead,
Einstein is dead,
And I ain't feeling great.

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